Salicylic acid degradation from aqueous solutions using Pseudomonas fluorescens HK44: parameters studies and application tools

Abstract

The optimal conditions for salicylic acid biodegradation by Pseudomonas fluorescens HK44 were determined in this study with the intention to create a microbial sensor. Kinetic experiments permitted a definition of 60 and 30min the time needed to achieve the maximum degradation of salicylic acid presented in a medium with and without yeast extract, respectively. The degradation in medium without yeast extract and the quantification by spectrophotometry 230 nm were selected to be used in further tests. The use of preactivated cells or on the exponential growth phase showed better salicylic acid degradation percentages when compared to nonactivated cells or on the stationary growth state. Finally, the best cellular concentration used on the salicylic acid degradation was 0,1 g.L -1. Strain HK44 shows to be capable of degrade salicylic acid presented in simple aqueous systems, making this strain a promising tool for the application on a luminescent microbial sensor.

Electro‐Fenton Oxidation of Salicylic Acid from Aqueous Solution: Batch Studies and Degradation Pathway

“…Salicylic acid (SA) and its analogs are commonly used as effective analgesics and are available to the public in a wide variety of formulations . The role of SA in the defense mechanisms against biotic and abiotic stress has been already reported .…”

Redox active films of salicylic acid-based molecules as pH and ion sensors for monitoring ionophore activity in supported lipid deposits

“…According to cyclic voltammetry and EIS of the electrodeposited SA-based redox active film, the maximal amount of deposited film is obtained at pH 4. This can be rationalized by the poor stability of SA in aqueous solutions, especially at pH above 5 [39][40] contrary to the greater stability of 5ASA at pH 2 to10 [25]. The most stable redox active film is however that obtained at pH 7 as shown by the relative retaining of the faradaic current after 500 consecutive cyclic voltammetry (Table S2).…”

Over-the-Counter Monocyclic Non-Steroidal Anti-Inflammatory Drugs in Environment—Sources, Risks, Biodegradation

“…If we take this into consideration, it is not strange that in nature, there are effective salicylate degradation mechanisms. Many bacterial strains, like Micrococcus , Sphingomonas , Amycolatopsis , Streptomyces , Pseudomonas , Alcaligenes , Pseudoramibacter , Rhodococcus (Chakrabarty 1972 ; Shamsuzzaman and Barnsley 1974 ; Haribabu et al 1984 ; Grund et al 1990 ; Grund et al 1992 ; Civilini et al 1999 ; Hintner et al 2001 ; Ishiyama et al 2004 ; Deveryshetty et al 2007 ; Jouanneau et al 2007 ; Silva et al 2007 ; Lanfranconi et al 2009 ) and fungi, like Sclerotinia , Trichosporon , Aspergillus , Fusarium , Rhodotorula , Cryptococcus (Anderson and Dagley 1980 ; Kuswandi and Roberts 1992 ; Middelhoven 1993 ; Iwasaki et al 2009 ; Qi et al 2012 ; Penn and Daniel 2013 ) are capable of degrading salicylate (Table 2 ) via a few catabolic pathways.…”

“…PPH SA ND Deveryshetty et al ( 2007 ) Sphingomonas sp. CHY-1 SA 0.5–1 mM Jouanneau et al ( 2007 ) Pseudomonas fluorescens HK44 SA 0.18–1.45 mM Silva et al ( 2007 ) Trichosporon cutaneum SA ND Anderson and Dagley ( 1980 ) Aspergillus nidulans SA 10 mM Kuswandi and Roberts ( 1992 ) Fusarium graminearum SA 0.1–20 mM Qi et al ( 2012 ) Sclerotinia sclerotiorum SA 1–10 mM Penn and Daniel ( 2013 ) Trichosporon moniliiforme WU-0401 SA 70 mM Iwasaki et al ( 2009 ) Rhodococcus spp. Paracetamol 1.65–3.31 mM Ivshina et al ( 2006 ) Delftia tsuruhatensis Paracetamol 0.007 mM de Gusseme et al ( 2011 ) Pseudomonas aeruginosa Paracetamol 0.007 mM de Gusseme et al ( 2011 ) Stenotrophomonas sp.…”