Comparison of an in-house and a commercial enzyme-linked immunosorbent assay (ELISA) for diagnosis of paratuberculosis

Abstract

An in-house PPA-ELISA was compared to a commercial ELISA using a panel of 108 serum samples. In relation to commercial test, in-house assay presented 100% of sensitivity and 83.5% of specificity and a high concordance (κ> 0.5), which demonstrates that the in-house assay is a valuable tool for use in developing countries.

An alternative for the preadsorption step in the paratuberculosis serodiagnosis: Mycobacterium fortuitum

“…All sera were tested by an ELISA test developed in our laboratory that uses protoplasmic paratuberculosis antigen (PPA) (8). This assay presented 100% sensitivity and 83.5% specificity, being comparable (κ >0.5) to commercial tests (9). Absorption of bovine sera was performed in three distinct ways: using M. phlei only, M. fortuitum only or a combination of M. phlei and M. fortuitum.…”