Keratinolytic bacteria isolated from feather waste

Riffel, Alessandro; Brandelli, Adriano

doi:10.1590/s1517-83822006000300036

Cited by 113 publications

(88 citation statements)

References 27 publications

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“…Microbe-induced feather degradation can potentially hinder flight (when wing feathers are involved) and mate attraction (when plumage condition or colour is important for mate selection), as well as thermoregulation [10,11,52]; although the extent and magnitude of such effects is currently unknown [24]. Keratinolytic microbes previously associated with birds include the bacterium Bacillus licheniformis [47] and the fungi Chrysosporium tropicum and Microsporum gallinae [27].…”

Section: Introductionmentioning

confidence: 99%

Intraspecific Variation and Interspecific Differences in the Bacterial and Fungal Assemblages of Blue Tit (Cyanistes caeruleus) and Great Tit (Parus major) Nests

Goodenough

Stallwood

2009

Microb Ecol

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Section: Introductionmentioning

confidence: 99%

Intraspecific Variation and Interspecific Differences in the Bacterial and Fungal Assemblages of Blue Tit (Cyanistes caeruleus) and Great Tit (Parus major) Nests

Goodenough

Stallwood

2009

Microb Ecol

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“…Plate assays were attempted on keratin powder plates by Nocardiopsis sp.TOA-1 where they formed extremely large ones on the plate (Mitsuiki et al, 2002). Milk agar medium was used for the primary screening of actinobacteria (Riffel and Brandelli, 2006). Another author discussed the primary screening of keratinolytic actinobacteria isolated from keratin waste dumped soil in Thiruchirapalli and Namakkal, Tamilnadu, India using milk agar medium (Saha and Dhanasekaran, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…Milk agar and soya meal agar medium were used for the primary screening of keratinolytic actinobacteria (Riffel and Brandelli, 2006). After sterilization, the media were poured into sterile Petri dishes.…”

Section: Primary Screening Of Keratinolytic Actinobacteriamentioning

confidence: 99%

Isolation and description of keratinase producing marine actinobacteria from South Indian Coastal Region

Selvam¹,

Vishnupriya²

2013

Afr. J. Biotechnol.

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A unique standard starch casein medium has been implemented for the isolation of actinobacteria from the south Indian marine sediments. A combination of techniques, morphological, physiological and biochemical tests provided the evidence for the isolated actinobacteria. All the 56 isolates were inoculated on milk agar and soya meal agar plates for the primary proteolytic screening and the proportional study was made by ANOVA. Among the 56 isolates, nine showed proteolytic activity in terms of making clear zone around their colony on the plates. Then, nine isolates were subjected to the secondary screening on feather broth where three isolates (IS -1, 2 and 18) showed degradation of feather between seven and ten days. The keratinolytic characters of crude enzymes were scrutinized by feather keratin as substrate and the protein concentration was determined. Then, the isolates were identified at molecular level by 16S rRNA gene amplification technique.

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“…It was clear that presence of feather in the growth medium act as an enzyme inducer. Riffel and Brandelli (2006) reported that keratinase producing bacteria had different characteristics, such as a broad temperature range of growth. The optimal proteolytic activities were detected between 30 and 37°C (Atalo and Gashe, 1993).…”

Section: Discussionmentioning

confidence: 99%

Biochemical and Molecular Characterization of a New Local Keratinase Producing Pseudomomanas sp., MS21

Tork¹,

Aly²,

Nawar³

2009

Asian J. of Biotechnology

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This study aimed to isolate and identify a new local bacterial strain, able to completely degrade keratin-rich wastes into soluble and useful materials which can be used in many proposes. Bacterial keratinases are of particular interest because of their action on insoluble keratin substrates and generally on a broad range of protein substrates. These enzymes have been studied for de-hairing processes in the leather industry and hydrolysis of feather and keratin. Samples from poultry industry wastes, soil, water, fodder and feather were collected from different places in Jeddah, Saudi Arabia. Each sample was plated on feather meal agar plates containing 5 g LG feather as the sole carbon and nitrogen source and 1 the obtained colonies were selected, purified and their growth were detected on skimmed milk agar and feather meal broth media. The well grown isolates on feather meal agar which producing the largest clearing zone on skimmed milk plate were selected for keratinase assays. Out of 23 bacterial isolates, 7 isolates were selected. The best keratinase producing bacterium kera MS21 was selected and identified based on morphological, physiological and some biochemical characteristics. It was recorded as a species belonging to the genus Pseudomonas and identified as Pseudomonas sp. The results of identification were confirmed by 16S rDNA studies. Precipitation and purification of the keratinase enzyme in addition to factors affecting enzyme activity were studied. The enzyme molecular weight was determined to be of 30 KDa using sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis. The optimum temperature and pH were determined to be 37°C and pH 8.0, respectively. The effect of some proteases inhibitors and activators were also studied.

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Keratinolytic bacteria isolated from feather waste

Cited by 113 publications

References 27 publications

Intraspecific Variation and Interspecific Differences in the Bacterial and Fungal Assemblages of Blue Tit (Cyanistes caeruleus) and Great Tit (Parus major) Nests

Intraspecific Variation and Interspecific Differences in the Bacterial and Fungal Assemblages of Blue Tit (Cyanistes caeruleus) and Great Tit (Parus major) Nests

Isolation and description of keratinase producing marine actinobacteria from South Indian Coastal Region

Biochemical and Molecular Characterization of a New Local Keratinase Producing Pseudomomanas sp., MS21

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