Isolation and description of keratinase producing marine actinobacteria from South Indian Coastal Region

Selvam, Kanesan Panneer; Vishnupriya, B.

doi:10.5897/ajb12.2428

Cited by 16 publications

(2 citation statements)

References 24 publications

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“…), or bacteria (e.g. Bacillus subtilis and Bacillus licheniformis ) (Gopinath et al, 2015;More, 2013;Selvam, Vishnupriya, & Yamuna, 2013). Feather degradation mainly depends on keretinase enzymes which are responsible for releasing peptides from keratin (Nickerson & Durand, 1963) after reduction of native keratine to reduced keratin by disulfite reductase enzyme (Sangali & Brandelli, 2000).…”

Section: Discussionmentioning

confidence: 99%

Characterization of Keratin Degrading Alkaliphiles

Elsayed

Mowafy

Elrazak

et al. 2016

JBLS

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Feather which mainly contains Keratin is one of the main poultry industry wastes and at the same time with high nutritional value if converted to soluble form. In this study, we isolated and characterized four alkaliphilic bacteria for their ability to degrade feather under alkaline conditions. Two of the isolates have been identified as Lysinibacillus spp. and both of them are growing optimally at 30 °C and pH 9. The others were identified as Micrococcus sp. and Nocardiopsis sp. and both of them showed optimum growth at 37 °C and pH 10.5. All the isolates are promising and potential tools that could be used for keratin degradation.

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Section: Discussionmentioning

confidence: 99%

Characterization of Keratin Degrading Alkaliphiles

Elsayed

Mowafy

Elrazak

et al. 2016

JBLS

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“…A new bacterium, Bacillus tropicus, capable of destroying feathers, was isolated and identifi ed in a soil sample from a sea duck farm in Beibu Creek in Guangxa (China) (Shen et al, 2022). Of 56 actinobacteria isolated from the southern coastal region of India, only three showed the ability to degrade feathers (Selvam et al, 2013).…”

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confidence: 99%

Bacteria of Deep-Sea Sediments of the Black Sea Breaking Down Keratin

Avdiyuk,

Ivanytsia,

Varbanets

et al. 2024

Mikrobiol. Z.

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Down and feather raw materials generated at food processing plants are among the main environmental pollutants. Most enterprises use burial and burning methods to deal with this waste, which negatively affects the environmental situation. The use of enzymatic hydrolysis by decomposer microorganisms is a promising and safe method for processing keratin waste. Earlier, it was shown that bacteria isolated from the bottom sediments of the Black Sea are active producers of elastases, fibrinogenases, and fibrinases. Therefore, the aim of this work was to evaluate the ability of bacteria isolated from the deep-sea sediments of the Black Sea to exhibit other types of proteolytic activity, in particular, to decompose hard-to-reach protein keratin. Меthods. The objects of the study were 20 cultures of bacteria isolated from deep-sea sediments of the Black Sea represented by the genera Bacillus, Metabacillus, Priestia, and Robertmurraya. The cultures were grown under conditions of submerged cultivation at 28 °C, with a nutrient medium stirring rate of 232 rpm for 4 days. For growth, a basic nutrient medium containing 0.5 % defatted chicken feathers as sole sources of carbon and nitrogen was used. The keratinase activity was assessed by UV absorption at 280 nm of hydrolysis products of keratin-containing materials. Protein was determined by the Lowry method, and caseinolytic (total proteolytic) activity was determined by the Anson method. Disulfide reductase activity was measured spectrophotometrically at 412 nm by evaluating the yellow sulfide formed during the reduction of 5,5’-dithiobis-(2-nitrobenzoic acid) (DTNB). Results. It was shown that the cultures of Metabacillus idriensis 2 and Robertmurraya siralis 57, out of twenty studied, did not grow on a nutrient medium with chicken feathers as the only source of carbon and nitrogen. The remaining 18 cultures exhibited varying degrees of keratinase activity (from 3 to 32 U/mL). The highest level of activity is characteristic of the culture Priestia megaterium 035 (32 U/mL). A study of the ability to break down casein showed that the level of total proteolytic (caseinolytic) activity of most cultures ranged from 0.015 U/mL to 0.14 U/mL. The highest total proteolytic activity was demonstrated by Bacillus pumilus A (0.3 U/mL) and Priestia megaterium 55 (0.24 U/mL) cultures, which also demonstrated high keratinase activity. The highest level of disulfide reductase activity was observed in Bacillus pumilus A (63.3 µmol/min), Bacillus subtilis 248 (62.0 µmol/min), and Priestia megaterium 035 (61.3 µmol/min), and the lowest in Bacillus licheniformis 249. Thus, from the deep-sea sediments of the Black Sea, we have isolated a number of active producers of keratinases, representatives of the two genera Bacillus and Priestia, which, after studying their physicochemical and catalytic properties, may turn out to be promising for practical application, in particular in the development of new technologies for the utilization of down and feather poultry farm waste.

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Versatility and commercial status of microbial keratinases: a review

Sharma

Devi

2017

Rev Environ Sci Biotechnol

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Isolation and description of keratinase producing marine actinobacteria from South Indian Coastal Region

Cited by 16 publications

References 24 publications

Characterization of Keratin Degrading Alkaliphiles

Characterization of Keratin Degrading Alkaliphiles

Bacteria of Deep-Sea Sediments of the Black Sea Breaking Down Keratin

Versatility and commercial status of microbial keratinases: a review

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