Evaluation of three enrichment broths and five plating media for Salmonella detection in poultry

Rall, Vera Lúcia Mores; Rall, Ricardo; Aragon, Lina Casale; Silva, Márcia Guimarães da

doi:10.1590/s1517-83822005000200009

Cited by 28 publications

(14 citation statements)

References 18 publications

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“…Multiple Salmonella enrichment and plating schemes were used here to facilitate detection of Salmonella with atypical phenotypic characteristics as previous studies (30,31,(48)(49)(50)(51) have shown that detection of certain serovars of Salmonella may be influenced by enrichment and plating media. In our study, the 14 Salmonella-positive samples where multiple serovars of Salmonella were isolated yielded 14 different serovars (Table 3).…”

Section: Resultsmentioning

confidence: 99%

“…No evident association was observed between serovars that have been reported as weak H 2 S producers and a specific scheme (Table 3). In this study, we employed one plating medium that does (XLD) and one that does not (CHROMagar) use the production of H 2 S as an indicator of Salmonella (49,52). Select strains of six of the serovars isolated here (Salmonella serovars Tennessee, Newport, Agona, Senftenberg, and Typhimurium and S. enterica subsp.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Distributions of Salmonella Subtypes Differ between Two U.S. Produce-Growing Regions

Strawn

Danyluk

Worobo

et al. 2014

Appl Environ Microbiol

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bSalmonella accounts for approximately 50% of produce-associated outbreaks in the United States, several of which have been traced back to contamination in the produce production environment. To quantify Salmonella diversity and aid in identification of Salmonella contamination sources, we characterized Salmonella isolates from two geographically diverse produce-growing regions in the United States. Initially, we characterized the Salmonella serotype and subtype diversity associated with 1,677 samples collected from 33 produce farms in New York State (NYS). Among these 1,677 samples, 74 were Salmonella positive, yielding 80 unique isolates (from 147 total isolates), which represented 14 serovars and 23 different pulsed-field gel electrophoresis (PFGE) types. To explore regional Salmonella diversity associated with production environments, we collected a smaller set of samples (n ‫؍‬ 65) from South Florida (SFL) production environments and compared the Salmonella diversity associated with these samples with the diversity found among NYS production environments. Among these 65 samples, 23 were Salmonella positive, yielding 32 unique isolates (from 81 total isolates), which represented 11 serovars and 17 different PFGE types. The most common serovars isolated in NYS were Salmonella enterica serovars Newport, Cerro, and Thompson, while common serovars isolated in SFL were Salmonella serovars Saphra and Newport and S. enterica subsp. diarizonae serovar 50:r:z. High PFGE type diversity (Simpson's diversity index, 0.90 ؎ 0.02) was observed among Salmonella isolates across both regions; only three PFGE types were shared between the two regions. The probability of three or fewer shared PFGE types was <0.000001; therefore, Salmonella isolates were considerably different between the two sampled regions. These findings suggest the potential for PFGEbased source tracking of Salmonella in production environments.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Distributions of Salmonella Subtypes Differ between Two U.S. Produce-Growing Regions

Strawn

Danyluk

Worobo

et al. 2014

Appl Environ Microbiol

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“…The bacteriological method used for culturing Salmonella spp., Salmonella Typhi and Salmonella Typhimurium was modifications of the methods of Rall, Rall, Aragon, and Silva (2005) and Tunung et al (2006). A 1 mL of pre-enriched BPW was transferred to 10 mL Selenite Cystine Broth (SC; Merck, Darmstadt, Germany) and incubated at 37 C for 24 h. A loopful of the broth culture was then plated onto CHROMagar Salmonella (CHROMagar Microbiology, Paris, France) and Xylose Lysine Deoxycholate Agar (XLD; Eiken Chemical Co., Tochigi, Japan).…”

Section: Plating Methodsmentioning

confidence: 99%

Simultaneous detection of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium in sliced fruits using multiplex PCR

et al. 2011

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a b s t r a c tThis study aimed to determine the prevalence and quantity of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium in sliced fruits from hawker stalls and hypermarkets in Malaysia. Analysis was carried out using the most probable number (MPN) e multiplex polymerase chain reaction (PCR) method. The prevalence of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium in 210 samples of sliced fruits examined were 23.3%, 7.6% and 3.8%, respectively with estimated quantity varying from 0 to 19 MPN/g. This study urged the authority to look into the biosafety of sliced fruits in Malaysia.

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“…Faecal samples were collected from patients prior to any antibiotic therapy. Samples were collected in labeled sterile universal containers containing glycerol saline and were transported to the Laboratory for bacteriological analysis within 4 hours [9].…”

Section: Sample Collectionmentioning

confidence: 99%

Antibiotic Resistance in Extended Spectrum Beta-Lactamases (Esbls) Salmonella Species Isolated from Patients with Diarrhoea in Calabar, Nigeria

Oghenevo¹,

Bassey²,

Yhiler³

et al. 2016

J Clin Infect Dis Pract

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Objective: This study seeks to evaluate the prevalence of Salmonella producing ESBLs strains by phenotypic methods and their profile of drug resistance amongst patients with diarrhoea due to gastroenteritis and enteric Fever in Calabar, Nigeria.Methods: Stool samples were collected from 256 patients in Calabar with diarrhoea due to enteric fever and gastroenteritis. They were examined for Salmonella infection. Isolation and detection of Salmonella species was done following the standard ISO 6579:2002/Amd2007 method. Modified double disc synergy and phenotypic confirmatory tests were used to determine ESBL-producing Salmonella species.Results: Salmonella isolates were recovered from 44 (17.2%) stool samples; 24 (9.4%) were ESBLs producers and 20 (7.8%) were non-ESBLs producers. Eight (33.3%) and 3 (15.0%) strains of both ESBL producing and non-ESBL producing respectively, demonstrated resistance against 7 of the 8 antibiotics used in this study. Resistance against 3rd generation cephalosporin was observed in 34 (77.3%) of the Salmonella strains against ceftazidime, 26 (59.1%) against cefotaxime while all 44 (100%) Salmonella strains were resistant against ceftriaxone. Conclusion:The presence of ESBL Salmonella amongst isolated strains should not be overlooked. We recommend continuous surveillance of antimicrobial resistant strains and the rational use antimicrobials agents.

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Evaluation of three enrichment broths and five plating media for Salmonella detection in poultry

Cited by 28 publications

References 18 publications

Distributions of Salmonella Subtypes Differ between Two U.S. Produce-Growing Regions

Distributions of Salmonella Subtypes Differ between Two U.S. Produce-Growing Regions

Simultaneous detection of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium in sliced fruits using multiplex PCR

Antibiotic Resistance in Extended Spectrum Beta-Lactamases (Esbls) Salmonella Species Isolated from Patients with Diarrhoea in Calabar, Nigeria

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