Production of hydrolytic enzymes by the plant pathogenic fungus Myrothecium verrucaria in submerged cultures

Moreira, Fabiana Guillen; Reis, Simone dos; Costa, Moacyr Lobo da; Souza, Cristina Giatti Marques de; Peralta, Rosane Marina

doi:10.1590/s1517-83822005000100002

Cited by 36 publications

(20 citation statements)

References 21 publications

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“…Different sources like plants, fungi and bacteria can be used for the production of pectinases. However, pectinase production from microorganism is preferred over plants because the plant produces potentially harmful material including phenolic compounds and endogenous enzyme inhibitor, difficult to isolate and limited quantity of enzyme is produced (MOREIRA et al, 2005). In addition to this microorganism are also preferable due to low cost production, able to manipulate genetically, the presence of great diversity in chemical and physical characteristic and culture development is fast independent from seasonal fluctuations.…”

Section: Introductionmentioning

confidence: 99%

Process optimization for pectinase production by locally isolated fungal strain using submerged fermentation

Abdullah¹,

Jafer²,

Nisar³

et al. 2018

Biosci. J.

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The present study deals with the isolation screening and optimization of fungal strain for pectinase production. The fungal strains were isolated from different sources, including soil, fruits etc. Qualitative screening was performed on the basis of the pectin hydrolysis zone. While, quantitative screening was carried out employing submerged fermentation. Among all the strains the strains showing highest pectinolytic potential were selected identified and assigned the code Aspergillus niger ABT-5.The influence of different fermentation media on pectinase production was evaluated. The M5 medium containing 10g wheat bran, nutrient medium containing (g/l) of (NH 4 ) 2 SO 4 6.0, K 2 HPO 4 6.0, KH 2 PO 4 6.0, MgSO 4 .7H 2 O 0.1 gave the highest pectinase production. The other important physico chemical parameters including incubation period, temperature, and volume of media, size of inoculum, carbon and nitrogen sources were also optimized for pectinase production. The highest pectinase production (15.5U/ml) was obtained at 72h of incubation, pH 6, temperature 30°C, volume of media 50ml. Fructose and urea were designated as best carbon and nitrogen sources subsequently.

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Section: Introductionmentioning

confidence: 99%

Process optimization for pectinase production by locally isolated fungal strain using submerged fermentation

Abdullah¹,

Jafer²,

Nisar³

et al. 2018

Biosci. J.

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“…Moreira et al . reported that Myrothecium verrucaria , a plant pathogenic fungus produces alkaline protease in the medium. Colletotrichum gloeosporioides , pathogen causing anthranose disease produces serine alkaline protease .…”

Section: Discussionmentioning

confidence: 99%

“…Nightingale et al [35] reported that alkaline protease activity correlates with the degradation of reserve proteins in wheat grain infected with F. culmorum. Moreira et al [31] reported that Myrothecium verrucaria, a plant pathogenic fungus produces alkaline protease in the medium. Colletotrichum gloeosporioides, pathogen causing anthranose disease produces serine alkaline protease [36].…”

Section: Discussionmentioning

confidence: 99%

Production, partial purification and characterization of protease from a phytopathogenic fungi Alternaria solani (Ell. and Mart.) Sorauer

Chandrasekaran

Sathiyabama

2013

J. Basic Microbiol.

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An alkaline serine protease producing strain Alternaria solani was optimized for its enzyme production under submerged conditions. The maximum production of protease by A. solani was achieved by using sodium nitrate at the optimum concentration of 0.2% w/v. A. solani produced higher quantities (3.75 [unit/mg of protein]) of an inducible extracellular proteases on day 9 after incubation in czapek's dox broth medium amended with 1% casein as an inducer at pH 8.5, temperature 27 °C and 3% sucrose as carbon source. Extracellular proteases were precipitated by ammonium sulphate saturation (80%) method and purified on Sephadex G-100 column chromatography. The molecular mass of SDS-PAGE and Sephadex G-100 Column Gel permeation chromatography purified protease was estimated to 42 kDa. In addition, trypsin digestion of 42 kDa protein band was carried out and analyzed by MALDI-TOF for the identification of protease. The sequence IKELATNGVVTNVK (378-391) segment of the alkaline serine protease was found by using MS/MS spectrum at 1485 m/z from the purified fraction. It showed optimal activity at 50 °C and pH 9-10 and broad pH stability between pH 6-12. The protease activity was inhibited by phenyl methyl sulfonyl fluoride (PMSF), all the results indicated that the presence of a serine residue in the active site and is thus most likely a member of the serine protease family. This may function as a virulence protein during pathogenesis by A. solani. The results suggested that the presence of appreciable extracellular proteolytic activity in filamentous fungi may serve as a marker of their phytopathogenicity.

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“…have been reported to elaborate high levels of cellulases [61]. The capability of the plant pathogenic fungus Myrothecium verrucari to produce extracellular enzymes in submerged culture has been reported [62].…”

Section: Mining Plant Pathogens For Cellulolytic and Xylanolytic Enzymesmentioning

confidence: 99%

Novel perspectives for evolving enzyme cocktails for lignocellulose hydrolysis in biorefineries

Saritha

Amat

Choudhary

et al. 2013

sustain chem process

134

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The unstable and uncertain availability of petroleum sources as well as rising cost of fuels have shifted global efforts to utilize renewable resources for the production of greener energy and a replacement which can also meet the high energy demand of the world. Bioenergy routes suggest that atmospheric carbon can be cycled through biofuels in carefully designed systems for sustainability. Significant potential exists for bioconversion of biomass, the most abundant and also the most renewable biomaterial on our planet. However, the requirements of enzyme complexes which act synergistically to unlock and saccharify polysaccharides from the lignocellulose complex to fermentable sugars incur major costs in the overall process and present a great challenge. Currently available cellulase preparations are subject to tight induction and regulation systems and also suffer inhibition from various end products. Therefore, more potent and efficient enzyme preparations need to be developed for the enzymatic saccharification process to be more economical. Approaches like enzyme engineering, reconstitution of enzyme mixtures and bioprospecting for superior enzymes are gaining importance. The current scenario, however, also warrants the need for research and development of integrated biomass production and conversion systems.

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Production of hydrolytic enzymes by the plant pathogenic fungus Myrothecium verrucaria in submerged cultures

Cited by 36 publications

References 21 publications

Process optimization for pectinase production by locally isolated fungal strain using submerged fermentation

Process optimization for pectinase production by locally isolated fungal strain using submerged fermentation

Production, partial purification and characterization of protease from a phytopathogenic fungi Alternaria solani (Ell. and Mart.) Sorauer

Novel perspectives for evolving enzyme cocktails for lignocellulose hydrolysis in biorefineries

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