Novel perspectives for evolving enzyme cocktails for lignocellulose hydrolysis in biorefineries

Saritha, M.; Amat, Dolamani; Choudhary, Jairam; Arora, Anju; Nain, Lata

doi:10.1186/2043-7129-1-15

Cited by 139 publications

(75 citation statements)

References 55 publications

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“…Strategies to improve enzymes include bioprocessing for superior key enzymes, mining plant pathogens for hydrolytic enzymes, and enzyme engineering of which the latter is of the most importance. It includes mutagenesis, DNA screening, and gene expression or overexpression [99,100]. Conventional cellulose enzymes operate at a temperature of around 50°C.…”

Section: Future Prospectsmentioning

confidence: 99%

Biomass processing into ethanol: pretreatment, enzymatic hydrolysis, fermentation, rheology, and mixing

Volynets

Ein‐Mozaffari

Dahman

2016

Green Processing and Synthesis

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Alternate energy resources need to be developed to amend for depleting fossil fuel reserves. Lignocellulosic biomass is a globally available renewable feedstock that contains a rich sugar platform that can be converted into bioethanol through appropriate processing. The key steps of the process, pretreatment, enzymatic hydrolysis, and fermentation, have undergone considerable amount of research and development over the past decades nearing the process to commercialization. In order for the commercialization to be successful, the process needs to be operated at high dry matter content of biomass, especially in the enzymatic hydrolysis stage that influences ethanol concentration in the final fermentation broth. Biomass becomes a thick paste with challenging rheology for mixing to be effective. As the biomass consistency increases, yield stress increases which limits efficiency of mixing with conventional stirred tanks. The purpose of this review is to provide features and perspectives on processing of biomass into ethanol. Emphasis is placed on rheology and mixing of biomass in the enzymatic hydrolysis step as one of the forefront issues in the field.

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Section: Future Prospectsmentioning

confidence: 99%

Biomass processing into ethanol: pretreatment, enzymatic hydrolysis, fermentation, rheology, and mixing

Volynets

Ein‐Mozaffari

Dahman

2016

Green Processing and Synthesis

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“…Several agro-industrial and forestry residues are of great interest as feedstocks for cellulosic ethanol production, such as in the case of sugarcane bagasse and short rotation clones of Eucalyptus sp., respectively [4]. Brazil produced 659 million tons of sugarcane in the 2014/2015 harvest season and this resulted in the accumulation of 92 million tons of sugarcane bagasse (14 % on dry basis) after its processing for sucrose and/or first generation ethanol [5,6].…”

Section: Introductionmentioning

confidence: 99%

Production of cellulosic ethanol from steam-exploded Eucalyptus urograndis and sugarcane bagasse at high total solids and low enzyme loadings

Chiarello

Ramos

Neves

et al. 2016

Sustain Chem Process

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Cellulosic ethanol is one of the most important biotechnological products to mitigate the consumption of fossil fuels and to increase the use of renewable resources for fuels and chemicals. By performing this process at high total solids (TS) and low enzyme loadings (EL), one can achieve significant improvements in the overall cellulosic ethanol production process. In this work, steam-exploded materials were obtained from Eucalyptus urograndis chips and sugarcane bagasse to be subsequently used for enzymatic hydrolysis at high TS (20 wt%) and relatively low EL (13.3 FPU g −1 TS of Cellic CTec3 from Novozymes). Also, the fermentability of their corresponding hydrolysates was tested using an industrial strain of Saccharomyces cerevisiae (Thermosacc Dry from Lallemand). Enzymatic hydrolysis of steam-treated E. urograndis reached 125 g L −1 of glucose in 72 h, while steam-treated bagasse gave yields 25 % lower. Both substrate hydrolysates were easily converted to ethanol, giving yields above 25 g L −1 and productivities of 2.3 g L −1 h −1 for eucalypt and 2.2 g L −1 h −1 for bagasse after only 12 h of fermentation. Under the conditions used in this study, sugarcane bagasse glucans showed the potential to boost the ethanol production from sugarcane culms by 31 %, from the 80 L t −1 of first generation to a total production of 105 L t −1. On the other hand, E. urograndis plantations are able to achieve cellulosic ethanol productivities of 2832.2 L ha −1 year −1 , which was 57.8 % higher than the projected value of 1794.5 L ha −1 year −1 that was obtained for sugarcane bagasse.

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“…Pada industri tekstil, selulase berperan pada proses biostoning bahan denim (jeans), biopolishing serat tekstil, mengurangi pewarnaan yang berlebih, dan meningkatkan kecerahan warna kain. Pada industri makanan, enzim ini dapat membantu meningkatkan hasil ekstraksi pati dan protein, melembutkan tekstur, dan memperbaiki kualitas produk kue dan roti, serta memperkuat rasa dan aroma dari senyawa volatile produkproduk berbahan dasar buah-buahan dan sayursayuran Mohanram et al, 2013). Kebutuhan selulase ini akan semakin meningkat jika suatu saat bioetanol dari limbah biomassa berkayu (cellulosic-ethanol), sebagai salah satu bahan bakar alternatif, diproduksi dalam skala besar (Banerjee, Scott-Craig and Walton, 2010).…”

Section: Pendahuluanunclassified

PENGARUH KONDISI KULTUR PADA AKTIVITAS SELULASE ISOLAT Pycnoporus sp. DAN Phlebiopsis sp. (EFFECTS OF CULTURE CONDITIONS ON CELLULASE ACTIVITIES PRODUCED BY Pycnoporus sp. AND Phlebiopsis sp.)

Agustini¹,

Irianto²,

Turjaman³

et al. 2017

J selulosa

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ABSTRAKPenelitian pengaruh media kultivasi, pH dan suhu inkubasi terhadap produksi enzim selulase-kompleks dari Pycnoporus sp. FORDACC-03452 dan Phlebiopsis sp. FORDACC-02482 yang ditumbuhkan di media dedak padi dan tongkol jagung dengan metode kultur padat pada variasi pH 4-7 dan suhu 30°C-45°C, telah dilakukan. Hasil memperlihatkan bahwa media dedak padi cenderung menginduksi produksi endo-β-1,4-glukanase dan selobiohidrolase, sedangkan media tongkol jagung menginduksi produksi β-glukosidase. Namun, campuran kedua substrat tersebut tidak menghasilkan aktivitas selulase yang lebih baik. Selulase-kompleks yang dihasilkan Pycnoporus sp. menunjukkan aktivitas lebih baik dibandingkan dengan yang diproduksi Phlebiopsis sp. Filtrat kasar Pycnoporus sp. menunjukkan aktivitas-spesifik endo-β-1,4-glukanase optimum pada pH 6, suhu 35°C (0,403 ± 0,010 IU/mg); selobiohidrolase pada pH 6, suhu 40°C (0,540 ± 0,020 IU/mg); dan β -glukosidase pada pH 4, suhu 30°C (0,022±0,001 IU/mg). Sementara, Phlebiopsis sp. menunjukkan aktivitas-spesifik endo-β-1,4-glukanase optimum pada pH 6, suhu 35°C (0,202 ± 0,005 IU/mg); selobiohidrolase pada pH 4, suhu 45°C (0,180 ± 0,002 IU/mg); dan β-glukosidase pada pH 6, suhu 45°C (0,007 ± 0,001 IU/mg). Rendahnya aktivitas β-glukosidase menyebabkan selulase-kompleks dari penelitian ini belum dapat menghidrolisis limbah lignoselulosa dengan sempurna dan kadar glukosa yang diperoleh masih rendah. Oleh karena itu, optimasi produksi selulasekompleks dari Pycnoporus sp. dan Phlebiopsis sp. masih perlu diteliti lebih lanjut.

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Novel perspectives for evolving enzyme cocktails for lignocellulose hydrolysis in biorefineries

Cited by 139 publications

References 55 publications

Biomass processing into ethanol: pretreatment, enzymatic hydrolysis, fermentation, rheology, and mixing

Biomass processing into ethanol: pretreatment, enzymatic hydrolysis, fermentation, rheology, and mixing

Production of cellulosic ethanol from steam-exploded Eucalyptus urograndis and sugarcane bagasse at high total solids and low enzyme loadings

PENGARUH KONDISI KULTUR PADA AKTIVITAS SELULASE ISOLAT Pycnoporus sp. DAN Phlebiopsis sp. (EFFECTS OF CULTURE CONDITIONS ON CELLULASE ACTIVITIES PRODUCED BY Pycnoporus sp. AND Phlebiopsis sp.)

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