In vitro characterization of gE negative bovine herpesvirus types 1.1 (BHV-1.1) and 1.2a (BHV-1.2a)

Abstract

This study aimed the in vitro growth characterization of a previously constructed Brazilian bovine herpesvirus 1.2a with a deletion in the glycoprotein E gene (BHV-1.2a gE -

Efficacy of a gE-deleted, bovine herpesvirus 1 (BoHV-1) inactivated vaccine

“…These vaccines have an advantage over the conventional vaccines because the serological response induced by vaccination can be differentiated from that induced by the wild type virus, allowing identification of wild type virus-infected animals in a herd. Glycoprotein E (gE) is one of such non-essential proteins (Rebordosa et al 1996, Franco et al 2002a, Spilki et al 2004). Although it is conserved among other members of the Herpesviridae family, the role of gE in the in vitro growth characteristics may vary in function of the virus species and the host cell (Balan et al 1994).…”

“…The gE complex in HHV-1 and HHV-3 is also important for in vitro cell-to-cell spread (Balan et al 1994, Dingwell et al 1995. In comparison to wild type viruses, gE negative recombinants (gE -) produce smaller plaque sizes in vitro, although the growth kinetics or penetration process of the virus seem not to be dependent on the presence of gE (Rebordosa et al 1996, Chowdhury et al 1999, Spilki et al 2004 In previous studies, we described the construction of a recombinant vaccine, based on a Brazilian BoHV-1 isolate, from which the gE gene was deleted. This virus was named 265gE -and evaluated in vaccination/challenge experiments (Franco et al 2002a).…”