Genetic variability within Fusarium solani specie as revealed by PCR-fingerprinting based on pcr markers

Abstract

Fusarium solani fungus (teleomorph Haematonectria haematococca) is of relevance for agriculture, producing a disease that causes significant losses for many cultivars. Moreover, F. solani is an opportunistic pathogen to animals and humans. The complexity associated to its correct identification by traditional methods justifies the efforts of using molecular markers for isolates characterization. In this work, three PCR-based methods (one PCR-ribotyping and two PCR-fingerprinting) were used to investigate the molecular variability of eighteen F. solani isolates from four Brazilian States, collected from different substrates. Genetic analysis revealed the intraspecific variability within the F. solani isolates, without any correlation to their geographical origin and substrate. Its polymorphism was observed even in the very conserved sequence of rDNA locus, and the SPAR marker (GTG)5 showed the highest polymorphism. Together, those results may contribute to understand the relation between fungal genetic variability and cultivars resistance phenotypes to fungal-caused diseases, helping plant-breeding programs.

Genetic variability of Fusarium solani and Fusarium oxysporum f. sp. passiflorae isolates from Pantanal, Amazon and Cerrado biomes of Mato Grosso, Brazil

“…In II.2 subgroup, only isolates from the Cerrado biome (4P and 5P) were grouped. The non-grouping of isolates as to geographical location corroborates with reports in the literature for F. solani in other pathosystems (Arruda et al, 2005;Brasileiro et al, 2004).…”

Distribution of organic metabolites after Fusarium wilt incidence in tomato (Solanum lycopersicum L.)

“…The susceptible reaction of a number of tomato genotypes in the current study is likely attributed to lack of these resistance mechanisms. Diverse Fusarium pathotypes cause enormous damage to crops (O'Donnell, 2000;Chehri, 2014 ;Brasileiro et al, 2004 ;Thatcher et al, 2012). Genetic information on plant response to certain pathotypes is available for some species (Sperschneider et al, 2015); however such information on tomato response to F. solani is minimal.…”

Use of molecular markers to compare Fusarium verticillioides pathogenic strains isolated from plants and humans

“…Using the same enzyme restriction, Brasileiro et al (2004) did not observe a cutting site for F. solani isolates. None of the enzymes that were used in this study was effective for separating clinical isolates from phytopathogenic isolates of F. verticillioides, and only HaeIII, HinfI, and MspI could be used to distinguish F. verticillioides from the F. solani isolate.…”

“…This can be explained by the supposition raised by several authors that the isolates of F. verticillioides that cause human mycoses may have been acquired in the field after traumatic exposure to plants that were decomposing in the soil during tillage or other such field operations (Montiel, 2004). Brasileiro et al (2004) used the EI1 primer to detect intraspecific polymorphisms among isolates of F. solani from different hosts and reported that some isolates may have represented a clonal lineage by means of forming a group, and that other isolates of the same species showed genetic differences based on the use of this primer.…”

“…Several techniques have been used to assess the intraspecific variability in F. verticillioides: restriction fragment length polymorphism-internal transcribed spacer (RFLP-ITS) from rDNA (Patiño et al, 2006;Dissanayake et al, 2009); random amplified polymorphic DNA (RAPD; Nagarajan et al, 2006;Singh et al, 2006;Bayraktar et al, 2008), inter-simple sequence repeat (ISSR; Luongo et al, 2007;Bayraktar et al, 2008); amplified fragment length polymorphism (AFLP; Reynoso et al, 2009); intron splice site primer (ISSP; Brasileiro et al, 2004).…”