Detection of virulence genes in Salmonella Enteritidis isolated from different sources

Oliveira, Sı́lvia Dias de; Rodenbusch, Carla Rosane; Michael, Geovana Brenner; Cardoso, Marisa Ribeiro de Itapema; Canal, Cláudio Wageck; Brandelli, Adriano

doi:10.1590/s1517-83822003000500042

Cited by 53 publications

(30 citation statements)

References 9 publications

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“…Our results for the virulence plasmid gene spvC were similar to those found by other authors (Oliveira et al 2003, Castilla et al 2006, Amini et al 2010). There are also other studies that have found lower frequencies for this gene in strains of avian origin (Okamoto et al 2009, Derakhshandeh et al 2013, Moussa et al 2013.…”

Section: Discussionsupporting

confidence: 93%

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Detection of virulence-associated genes in Salmonella Enteritidis isolates from chicken in South of Brazil

et al. 2013

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RESUMO.-[Detecção de genes associados à virulência em cepas de Salmonella Enteritidis isoladas de frangos na região sul do Brasil.] Salmonella spp. estão entre os principais agentes causadores de doenças transmitidas por alimentos, e o sorovar Salmonella Enteritidis é o mais frequentemente isolado no mundo. A virulência de Salmonella spp. e a sua interação com o hospedeiro são processos complexos que envolvem fatores de virulência para sobreviver às defesas do hospedeiro. O objetivo deste estudo foi detectar genes de virulência em cepas de S. Enteritidis isoladas a partir de fontes avícolas no sul do Brasil. Ensaios de PCR foram desenvolvidos para a detecção de nove genes (lpfA, agfA, sefA, invA, hilA, avrA, sopE, sivH are considered the main agents of foodborne disease and Salmonella Enteritidis is one of the most frequently isolated serovars worldwide. The virulence of Salmonella spp. and their interaction with the host are complex processes involving virulence factors to overcome host defenses. The purpose of this study was to detect virulence genes in S. Enteritidis isolates from poultry in the South of Brazil. PCR-based assays were developed in order to detect nine genes (lpfA, agfA, sefA, invA, hilA, avrA, sopE, sivH and spvC) associated with the virulence in eighty-four isolates of S. Enteritidis isolated from poultry. The invA, hilA, sivH, sefA and avrA genes were present in 100% of the isolates; lpfA and sopE were present in 99%; agfA was present in 96%; and the spvC gene was present in 92%. It was possible to characterize the isolates with four different genetic profiles (P1, P2, P3 and P4), as it follows: P1, positive for all genes; P2, negative only for spvC; P3, negative for agfA; and P4, negative for lpfA, spvC and sopE. The most prevalent profile was P1, which was present in 88% of the isolates. Although all isolates belong to the same serovar, it was possible to observe variations in the presence of these virulence-associated genes between different isolates. The characterization of the mechanisms of virulence circulating in the population of Salmonella Enteritidis is important for a better understanding of its biology and pathogenicity. The frequency of these genes and the establishment of genetic profiles can be used to determine patterns of virulence. These patterns, associated with in vivo studies, may help develop tools to predict the ability of virulence of different strains.

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Section: Discussionsupporting

confidence: 93%

“…The virulence of Salmonella spp. is associated with a combination of chromosomal and plasmid factors (Oliveira et al 2003), and many studies have identified genes that encode these factors. Some virulence factors are associated with the cellular structure of the bacteria, such as fimbriae (Edwards & Puente 1998).…”

Section: Introductionmentioning

confidence: 99%

Detection of virulence-associated genes in Salmonella Enteritidis isolates from chicken in South of Brazil

et al. 2013

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“…The combination of the results described above with those obtained in previous studies for phage typing, presence of virulence genes and antimicrobial resistance pattern (23,24,31) permitted the identification of a total of 48 types (Table 1). Types 1, 2, 7, 8 and 9 were the most prevalent, being found in 56 isolates.…”

Section: Combination Of the Typing Methodsmentioning

confidence: 59%

“…These genes were detected by PCR as previously described for other S. Enteritidis strains that belonged to this study (24).…”

Section: Detection Of Virulence Genesmentioning

confidence: 99%

Phenotypic and genotypic characterization of Salmonella Enteritidis isolates

Oliveira¹,

Bessa²,

Santos³

et al. 2007

Braz. J. Microbiol.

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In order to study the epidemiology of Salmonella Enteritidis outbreaks and determine the source of contamination so that a recurrence can be avoided, detailed characterization is necessary. Thus, the purpose of this study was to verify whether rep-PCR was able to discriminate among Salmonella Enteritidis isolates. Phage typing, detection of virulence genes and antimicrobial resistance testing were also associated to rep-PCR results. One hundred and two S. Enteritidis isolates from broiler carcasses, food, human, pigs, poultryrelated samples, and nine isolates from other countries were genotypically typed by REP-PCR, ERIC-PCR and BOX-PCR, collectively called rep-PCR. Phage typing, detection of virulence genes and antimicrobial resistance testing were also performed. Only three fingerprinting profiles were obtained with each rep-PCR method, with the majority of isolates belonging to the same profile. No relationship was observed between genotypic profile and year, place of isolation or source of infection. However, the less frequent rep-PCR profiles showed single antimicrobial resistance patterns. Although few strains isolated from swine were analyzed, different antimicrobial resistance patterns were observed. Furthermore, phage type 4 was not found in swine isolates. rep-PCR showed a lower discriminatory power as compared with antimicrobial resistance and phage typing, but the combination of genotypic and phenotypic methods was more discriminatory than any method alone, resulting in 48 different types.

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“…Dentre portanto, é um importante objeto de estudo para a detecção desse microrganismo (OLIVEIRA et al, 2002;OLIVEIRA et al, 2003;SALEHI et al, 2005;AMINI et al, 2010).…”

Section: Avaliação Da Produção De Biofilme "In Vitro"unclassified

Avaliação da capacidade de produção de biofilmes e detecção da enzima KPC em Salmonella spp. isoladas de aviário e linha de abate de aves

Marquezini¹

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Detection of virulence genes in Salmonella Enteritidis isolated from different sources

Cited by 53 publications

References 9 publications

Detection of virulence-associated genes in Salmonella Enteritidis isolates from chicken in South of Brazil

Detection of virulence-associated genes in Salmonella Enteritidis isolates from chicken in South of Brazil

Phenotypic and genotypic characterization of Salmonella Enteritidis isolates

Avaliação da capacidade de produção de biofilmes e detecção da enzima KPC em Salmonella spp. isoladas de aviário e linha de abate de aves

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