Genetic analysis of Escherichia coli strains carrying enteropathogenic Escherichia coli (EPEC) markers, isolated from children in Rio de Janeiro city, Brazil

Régua-Mangia, Adriana Hamond; Gomes, Tânia A. T.; Andrade, João Ramos Costa; Vieira, Mônica A. M.; Gonzalez, Alice Gonçalves Martins; Zahner, Viviane; Irino, Kinue; Teixeira, Lúcia Martins

doi:10.1590/s1517-83822003000500013

Cited by 4 publications

(4 citation statements)

References 9 publications

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“…The study included 86 E. coli strains comprising 67 DEC strains [27 classified as EAEC, 21 as enteropathogenic E. coli (EPEC) and 19 as enterotoxigenic E. coli (ETEC)], and 19 UPEC strains, which were isolated and characterized according to genotypic and phenotypic traits during previous surveys conducted in Rio de Janeiro city, Brazil [8,15,30,31].…”

Section: Bacterial Strainsmentioning

confidence: 99%

Molecular characterization of uropathogenic and diarrheagenic Escherichia coli pathotypes

Régua-Mangia

Irino

Pacheco

et al. 2010

J. Basic Microbiol.

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In this study diarrheagenic and uropathogenic Escherichia coli (UPEC) strains were comparatively characterized according to serotype, hemolytic activity, protein polymorphism among housekeeping enzymes, phylogenetic group and urovirulence genes. Intra-serogroup/serotype variations were observed. Hemolytic activity was detected in 100%, 93%, 67% and 39% of UPEC, EAEC, EPEC and ETEC strains, respectively. The alpha-hemolytic phenotype was observed in all pathogenic groups while beta-hemolytic phenotype was less frequent. PCR phylotyping revealed higher prevalence of diarrheagenic E. coli in groups A and D while uropathogenic strains were mainly found in subgroup B2. Amplification assays revealed that 74%, 45% and 22% of UPEC, EAEC and EPEC strains, respectively, carried at least one of the urovirulence sequences. The molecular typing system revealed a pathotype-specific clonal group distribution and showed a closer relationship between the EAEC and UPEC. Additionally, the occurrence of urovirulence traits, especially those related to iron acquisition, was more frequent among EAEC and UPEC than among the other E. coli pathotypes. This observation is of special value considering that the EAEC pathotype constitutes an emerging group of enteropathogens, particularly, in developing countries, and information on their pathogenic and phylogenetic characteristics is still scarce.

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Section: Bacterial Strainsmentioning

confidence: 99%

Molecular characterization of uropathogenic and diarrheagenic Escherichia coli pathotypes

Régua-Mangia

Irino

Pacheco

et al. 2010

J. Basic Microbiol.

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“…Several molecular approaches mainly based on genetic techniques have been successfully applied in order to assess the clonal nature and variability within species [23,36]. The occurrence of distinct patterns of E. coli phylogenetic distribution provides evidence of both vertical and horizontal transmission [37][38][39]. The mechanisms of genetic diversification contribute to E. coli evolution and creation of new variants, as this bacterial species is often subjected to DNA rearrangements, excisions, transfers, and acquisitions [37,40].…”

Section: Random Amplification Of Polymorphic Dna (Rapd-pcr)mentioning

confidence: 99%

“…There are several highly adapted clones that have acquired specific virulence elements which confer an increased ability to adapt to new niches. Such plasticity may confer ability to acclimate environmental bacteria to new niches allowing these microorganisms to become members of microbial communities in a variety of environments, even facing conditions very different from their primary habitat [36,38,39,41]. RAPD-PCR approach was used to investigate the overall chromosomal relatedness among merA+ strains and revealed a high genetic diversity population suggesting that mercury resistance is widely dispersed in E. coli.…”

Section: Random Amplification Of Polymorphic Dna (Rapd-pcr)mentioning

confidence: 99%

Diversity of Mercury Resistant Escherichia coli Strains Isolated from Aquatic Systems in Rio de Janeiro, Brazil

Rebello

Gomes

Duarte

et al. 2013

International Journal of Biodiversity

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Escherichia coli may harbor genetic mercury resistance markers which makes this bacterial species a promising alternative for bioremediation processes. The objective of this study was to investigate phenotypic and genetic characteristics related to diversity and mercury resistance among 178 Escherichia coli strains isolated from residential, industrial, agricultural, and hospital wastewaters and recreational waters at Rio de Janeiro city. Genetic and conventional methods were carried out in order to determine mercury resistance. Random amplification of polymorphic DNA (RAPD-PCR) and denaturing gradient gel electrophoresis (DGGE) were used to investigate genetic variability. RAPD data revealed a high degree of polymorphism among E. coli mercury resistant strains and showed reproducibility and good discriminative results. DGGE typing detected diversity within the merA gene fragment. Our findings represent an improvement in epidemiological studies of Hg R E. coli and support the evidence of nonclonal nature of mercury resistant E. coli strains circulating in rural and urban aquatic systems in Rio de Janeiro city.

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“…Pulsed field gel electrophoresis (PFGE) is considered one of the most reliable, discriminatory, and reproducible typing method, allowing the detection of a high degree of DNA polymorphism (18) for epidemiological purposes (15,18,19,21) and in routine typing of Shigella species (3).…”

Section: Introductionmentioning

confidence: 99%

Molecular epidemiology of Shigella spp strains isolated in two different metropolitam areas of southeast Brazil

Angelini¹,

Stehling²,

Moretti³

et al. 2009

Braz. J. Microbiol.

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Shigella spp., the human pathogen responsible for shigellosis, is highly infectious even at low levels. The incidence rate of shigellosis varies with geographical distribution, location human development index, and age groups, being higher among children aged under 5 years. In Brazil, a few works indicate that shigellosis cases are underestimated, with S. flexneri and S. sonnei strains being the major agents responsible for the shigellosis cases. The present study used pulsed field gel electrophoresis (PFGE) to investigate the molecular epidemiology of 119 strains of S. sonnei and S. flexneri isolated from shigellosis cases that occurred in the metropolitan areas of Ribeirão Preto and Campinas Cities, São Paulo Sate, southeast Brazil. The results indicated (i) the existence of just a few strain clusters for both species, but with genotype variability with either a high speed of genetic change or constant introduction of several genotypes, considering the intense migration to these two metropolitan areas, and (ii) the prevalence of specific genotypes in each geographical area, which suggests the successful adaptation of some genotypes to the local environmental conditions. Our results indicate the need of more efficacious sanitary barriers to prevent Shigella spp. outbreaks and epidemics.

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Genetic analysis of Escherichia coli strains carrying enteropathogenic Escherichia coli (EPEC) markers, isolated from children in Rio de Janeiro city, Brazil

Cited by 4 publications

References 9 publications

Molecular characterization of uropathogenic and diarrheagenic Escherichia coli pathotypes

Molecular characterization of uropathogenic and diarrheagenic Escherichia coli pathotypes

Diversity of Mercury Resistant Escherichia coli Strains Isolated from Aquatic Systems in Rio de Janeiro, Brazil

Molecular epidemiology of Shigella spp strains isolated in two different metropolitam areas of southeast Brazil

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