Production of hydrolytic enzymes by Trichoderma isolates with antagonistic activity against Crinipellis perniciosa, the causal agent of witches' broom of cocoa

Abstract

Two isolates of Trichoderma, which reduce the incidence of witches'broom disease caused in cocoa by Crinipellis perniciosa, were evaluated for their potential to produce hydrolases in liquid medium. Very low or no hydrolytic activity was produced in the absence of any substrate. The activities of chitinase, N-acetylglucosaminidase, β-1,3-glucanase, total cellulase, endoglucanase, aryl-β-glucosidase, β-glucosidase, protease and amylase increased dramatically within 72-120 h of growth in the presence of specific substrates. Except for N-acetylglucosaminidase and β-glucosidase Trichoderma harzianum isolate 1051 produced the largest amounts of hydrolases. The possible involvement of these enzymes in the antagonistic interaction between Trichoderma and C. perniciosa is discussed.

Antagonism of Trichoderma spp. against Macrophomina phaseolina: Evaluation of Coiling and Cell Wall Degrading Enzymatic Activities

“…It indicated that β-1, 3 glucanase inhibited the growth of pathogen in synergistic cooperation with chitinase [35,40]. Similar to present study, Marco et al [12] noted that two isolates of T. harzianum (39.1 and 1051) produced and secreted on induction of substantial amounts of chitinolytic enzymes and it increased within 72 h, while β-1, 3 glucanase activity was found to be maximum during 72 to 120 h growth in presence of specific substrate.…”

“…[11]. For enzyme induction in pathogen M. Phaseolina (Control), 1% of either chitin, laminarin, casein or carboxymethyl cellulose were added into liquid culture (SM) and pH adjusted to 5.5 [12]. Cultures were then incubated at 30°C in a rotary shaker at 120 rpm for 96 h. After 24, 48, 72 and 96 h time interval, 5…”

Chitinolytic efficacy and secretion of cell wall degrading enzymes from Trichoderma spp. in response to phyto-pathological fung

“…Marco et al [53] also noted similar results that two different T. harzianum (39.1 and 1051) induce a remarkable level of chitinolytic enzymes during 72 h, whereas β-1, 3 glucanase activity induced during 72-120 h growth with substrate.…”

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“…harzianum 1051 growing in casein-containing liquid medium produces substantial proteolytic activity as previously reported [20]. The supernatant of T. harzianum 1051 cultures were then used to purify the protein showing proteolytic activity against casein.…”