Leptospirosis in slaughtered sows: serological and histopathological investigation

Delbem, Alberto Carlos Botazzo; Freitas, Júlio César de; Bracarense, Ana Paula Frederico Rodrigues Loureiro; Müller, Ernst Eckehardt; Oliveira, Renata Cristina Gobbi de

doi:10.1590/s1517-83822002000200016

Cited by 14 publications

(17 citation statements)

References 11 publications

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“…Similar findings were reported by Freitas et al (15). The animal 10K presented congestion of uterine horns and focuses of mononuclear cells in its genital tract, as described by Delbem et al (5) and Schönberg et al (55).…”

Section: Number Of Pigs With Pcr Positivesupporting

confidence: 85%

“…Histopathology by the hematoxilin-eosin and Warthin-Starry staining has been used to demonstrate leptospires and structural lesions in affected organs, although there is no association between them, as the lesions are usually non specific (5,42,48,53,56). After isolation in appropriate culture media, the bacterium must be characterized by serological methods for serogroup and serovar determination (6,31).…”

Section: Introductionmentioning

confidence: 99%

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Isolation and characterization of Leptospira interrogans from pigs slaughtered in São Paulo State, Brazil

Miraglia¹,

Moreno²,

Gomes³

et al. 2008

Braz. J. Microbiol.

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With the aim of isolating Leptospira spp., blood serum, kidney, liver and genital tract of 137 female swine (40 sows and 97 gilts) and also urine samples from 22 sows were collected in a slaughterhouse in the State of São Paulo, from April 2003 to August 2004. Four isolates were obtained from animals that presented microagglutination test (MAT) titers ≥ 100 for the serovar Pomona and one was obtained from an animal negative by MAT in which Leptospira was isolated from the liver and reproductive tract. The presence of leptospiral DNA was investigated by PCR, and positive results were found in kidneys of 11 females, liver of two, genital tract of two and urine of one of them. Nephrosis, interstitial multifocal nephritis, moderate to severe changing, hyalines cylinders and hemorrhagic focuses, hepatic and uterine horns congestion were histological lesions observed in higher frequency in animals positive for leptospira. The silver impregnation (Warthin Starry) confirmed the presence of spirochetes in renal tubules of four females with positive leptospira cultures from kidneys. The serogroup of the five isolates was identified as Pomona by cross agglutination with reference polyclonal antibodies. Molecular characterization of the isolates was carried out by variablenumber tandem-repeats analysis. All the isolates revealed a pattern distinct from the L. interrogans Pomona type strain, but identical to a previously identified pattern from strains isolated in Argentina belonging to serovar Pomona.

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Section: Number Of Pigs With Pcr Positivesupporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of Leptospira interrogans from pigs slaughtered in São Paulo State, Brazil

Miraglia¹,

Moreno²,

Gomes³

et al. 2008

Braz. J. Microbiol.

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“…A coleção de antígenos utilizada no presente estudo não continha tal sorovariedade. É possível que tenham ocorrido reações cruzadas e que Bananal esteja presente infectando populações em áreas pantaneiras, posto que a presença de sorovariedades autóctones não são raras e já foram relatadas em estudos anteriores (SANTA ROSA; SULZER; CASTRO, 1972;VASCONCELLOS et al, 2001;DELBEM et al, 2002;MARVULO et al, 2002;CORREA et al, 2004;FREI-TAS et al, 2004;OLIVEIRA et al, 2005;AHMED et al, 2006;FAGUNDES et al, 2008;MIRAGLIA et al, 2008;ZACARIAS et al, 2008).…”

Section: Metodologiaunclassified

“…Freitas et al (2004) isolaram a estirpe LO-1 da urina de um cão da cidade de Londrina, PR, tipificada como sorogrupo Canicola sorovariedade Canicola (OLIVEIRA et al, 2005;AHMED et al, 2006;FAGUNDES et al, 2008). Delbem et al (2002) isolaram estirpe L04 do fígado de um suíno, tipificada como pertencente ao sorogrupo Canicola sorovariedade Canicola (FREITAS et al, 2004;FAGUNDES et al, 2008). Zacarias et al (2008) isolaram a estirpe L014 da urina de um bovino abatido no estado do Paraná, tipificada como pertencente ao sorogrupo Canicola sorovariedade Canicola (AHMED et al, 2006).…”

Section: Introductionunclassified

Pesquisa de leptospiras e de anticorpos contra leptospiras em animais e humanos de propriedades rurais nos biomas brasileiros Pantanal e Caatinga

Silva

Santos

Silva

et al. 2015

Braz. J. Vet. Res. Anim. Sci.

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The occurrence of Leptospira and of seroreactivity against Leptospira was investigated in animals and humans from six farms located in two Brazilian biomes that have different geoclimatic conditions: Pantanal -municipalities of Miranda (MS), Itiquira (MT) and Poconé (MT) and Caatinga -municipalities of Sobradinho (BA), Garanhuns (PE) and Sobral (BA). Blood and urine samples of wildlife, domestic animals and humans were collected at each property. The samples were collected from February to April 2012 in Caatinga and from July to September 2012 in Pantanal. The serological reactivity against Leptospira spp. was verified by microscopic agglutination technique (MAT) made with a collection consisting by 24 antigens of Leptospira spp. The leptospires research was carried out by urine samples crop sown in Fletcher resources and Ellinghausen -McCullough -Johnson -Harris (EMJH). Crops with growth of leptospires were referred to the Leptospirosis Laboratory of the Institute of Pathobiology, National Institute of Agricultural Technology, Buenos Aires, Argentina and isolated Leptospira strains were genotyped with the technique of Multiple Locus Variable Number Tandem Repeat Analysis (MLVA). The classification procedure employed the VNTR 4, 7, 9, 10, 19, 23, 31, LB4 and LB5, which discriminate strains of L. interrogans and L. borgpetersenii. In Pantanal, 17 wildlife, 65 domestic animals and two humans were examined. In Caatinga, seven wild animals were examined, along with 100 domestic animals and 26 humans. Of 84 blood samples tested in Pantanal, 47 (55.95%) were positive and, of 133 in Caatinga, 59 (44.36%) were reactant. By Fisher's exact test, considering a 0.05 significance level, there was no difference between the proportions of serum reagent animals against Leptospira spp. in two biome reviews (p = 0.063). The predominant serovars in SAM reactions were: 1) Pantanal -Bratislava (wildlife, dogs and humans), Grippotyphosa (horses and cattle); 2) Caatinga -Copenhageni (humans and dogs), Patoc (horses and cattle), Panama (sheep and goats), Patoc, Copenhageni and Australis (wildlife). Four strains of Leptospira were isolated: two in Sobradinho, BA, L. interrogans serogroup Pomona in Cavea aperea and L. interrogans in Euphractus sexcinctus; and two in Sobral, CE, L. interrogans in Cerdocyon thous and L. interrogans serogroup Pomona in Euphractus sexcinctus.

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“…Quando um animal apresentou título mais elevado idêntico para dois ou mais sorovares o mesmo foi descartado desta análise Amsterdan, Holanda, a tipificação foi efetuada por painel de anticorpos monoclonais, caracterizando a estirpe como sorovar Copenhageni semelhante à estirpe de referência M20 (CORREA et al, 2004, FAGUNDES et al, 2008. DELBEM et al, 2002;FREITAS et al, 2004;FAGUNDES et al, 2008). …”

Section: Leitura E Interpretaçãounclassified

Emprego de estirpes de leptospiras isoladas no Brasil, na microtécnica de soroaglutinação microscópica aplicada ao diagnóstico da leptospirose em rebanhos bovinos de oito estados brasileiros

Sarmento¹

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Ao meu querido orientador, professor doutor Silvio Arruda Vasconcellos, que além de ser o mentor deste trabalho, é exemplo de um grande docente e profissional, agradeço a dedicação, paciência, carinho e amizade de um verdadeiro pai, que aconselhou e ajudou nos momentos mais difíceis, no desenvolvimento deste trabalho e na vida pessoal.Á professora doutora, Sônia Regina Pinheiro, minha iniciadora deste desencanto, abriu meus olhos, me guiou para uma luz com muito brilho, mostrou os caminhos, talvez longos, mas corretos e onde pude crescer profissionalmente. "Amigo é coisa para se guardar debaixo de sete chaves, dentro do coração, assim falava a canção." Sem você, eu não estaria aqui e este trabalho não existiria. Obrigada por tudo, você é uma pessoa muito especial, uma verdadeira amiga. Às técnicas do Laboratório de Zoonoses Bacterianas do Departamento de Medicina Veterinária Preventiva e Saúde Animal da FMVZ-USP, Zenáide Maria de MoraisHiga, uma verdadeira mãe, que afaga, dá aconchego, calor e carinho e a Gisele Oliveira de Souza, obrigada pelo seu entusiasmo, uma pessoa muito carismática.Obrigada por todo apoio técnico e pessoal. Em especial, a técnica do Laboratório de Epidemiologia e Estatística doDepartamento de Medicina Veterinária Preventiva e Saúde Animal da FMVZ-USP, Jucélia de Jesus Pereira, um coração grande que irradia tanta luz e amor, que quase não cabe dentro do seu peito. Obrigada pela força pessoal e técnica na preparação dos mapas e gráficos, na orientação final desta dissertação. Aos meus colegas pós-graduandos em especial a Adriana, Iara e Isabelle, companheiras de ombro amigo, onde pudemos partilhar as magoas e as alegrias.Aos meus colegas de leptospirose e de laboratório, a Flávia Carolina de Oliveira, obrigada pela esta energia forte e irradiante de uma verdadeira baiana e amiga, obrigada pela ajuda nesta dissertação, com saúde, muito amor e paz, vivamos felizes, cada instante de nossas vidas. A Amane Paldes Gonçales, obrigada pela ajuda na parte prática deste trabalho e pela experiência passada em laboratório, ao A toda equipe da biblioteca da FMVZ -USP, Elza agradeço pela correção da dissertação e pela sua receptividade carinhosa, agradecimento especial a Solange que me acompanhou nesta caminhada da pesquisa na biblioteca, sempre atenciosa e prestativa e a Fernanda e Rosangela. Obrigada. Among the 9,820 examined animals, 5,806 (59.12%) were reactants to the MAT for any serovar using the 23 reference serovars. Using the collection of reference serovars and the ten autochthonous strains there were 6,400 (65.24%) reactants, and the difference found was significant (p=0.001). The only state with non-significant results in the number of reactants for any serovar was the State of Santa Catarina (p=0.522). Of the 109 properties analyzed, 106 were considered positive at least with one reactant animal by MAT. According to the antigens used, there was no significant difference among the properties. The most probable serovars identified by the collection of reference antigens were Hardjo (43.03%), ...

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Leptospirosis in slaughtered sows: serological and histopathological investigation

Cited by 14 publications

References 11 publications

Isolation and characterization of Leptospira interrogans from pigs slaughtered in São Paulo State, Brazil

Isolation and characterization of Leptospira interrogans from pigs slaughtered in São Paulo State, Brazil

Pesquisa de leptospiras e de anticorpos contra leptospiras em animais e humanos de propriedades rurais nos biomas brasileiros Pantanal e Caatinga

Emprego de estirpes de leptospiras isoladas no Brasil, na microtécnica de soroaglutinação microscópica aplicada ao diagnóstico da leptospirose em rebanhos bovinos de oito estados brasileiros

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