Chemical and microbiological evaluation of the internal surfaces of aluminum tubes both unlined and lined with epoxi resin by means of the stereoscope and scanning electron microscope

Abstract

The internal surfaces of aluminum tubes, both unlined and lined with epoxy resin, which are used for packaged medicines such as creams, ointments, gels, etc., were evaluated chemically, and microbiologically with regard to microorganism adherence. Proof of porosity of the unlined aluminum surface and lined with epoxy resin, and the resistance to removal of the resin were observed using a scanning electron microscope and a stereoscope, with a digitalized image system. To evaluate the microorganisms adherence to the aluminum surface, unlined and lined with epoxy resin, sterilized disks (10mm in diameter) were immersed in Mueller Hinton Broth and placed in tubes of polypropylene with screw caps. The liquid culture medium was then inoculated with suspensions (10 9 CFU/ml) of the following microorganisms: Streptococcus agalactiae, Staphylococcus aureus, Acinetobacter lwoffii and Candida albicans and incubated at 37°C under constant agitation (100rpm) for 12 days. The culture broth was changed every 3 days, after which the disks were removed, and observed under the scanning electron microscope. Microscopic observations revealed adherence of the microorganisms and biofilm on the aluminum surfaces both unlined and lined with epoxy resin.

Impact of the contamination time by Escherichia coli on biofilm formation in surgical instruments

“…The study was conducted according to the reference methodology published by Bjerka et al and Pizzolito et al (15)(16) . To ensure sterility, the fragments were submitted to automated cleaning in ultrasonic washer (USC-2800, Engesolutions ® , São Paulo, Brazil) (40 kHz), rinsed in distilled water, placed on absorbent paper for drying, packed in SMS (Spunbonded/Meltblow/Spunbonded) and sterilized by pressure-saturated steam (CS, Prismatec, Itu, São Paulo) at 121°C, for 15 minutes.…”

Aderência bacteriana: estudo in vitro de superfície de aço inoxidável e liga de titânio-alumínio-vanádio de uso ortopédico

“…Staphylococcus epidermidis em discos de liga de Ti-6Al-4V ASTM F-136 e aço inoxidável ASTM F-138 (Pizzolitto et al,2001) Da suspensão bacteriana da ordem de 10 8 UFC/mL, uma alíquota de 0,2 mL de cada suspensão bacteriana foi transferida individualmente para tubos cônicos de poliestireno (Falcon), esterilizados, contendo 15mL de caldo Mueller Hinton e um corpo-de-prova foi introduzido assepticamente em cada tubo com o inóculo bacteriano.…”