Immunoblot profiles of sera from laboratory rats naturally infected with Mycoplasma pulmonis and technicians exposed to infected animal facilities

Detection of Mycoplasma pulmonis in Laboratory Rats and Technicians

“…This amount can be in particles generated during rat sneezing in such facilities by infected animals and favors the transmission of this bacterium to healthy animals and humans (technicians). The culture of this agent from a human oropharynx is difficult because there are other mycoplasmas species and bacteria in the normal flora (Delgado and Timenetsky, 2001).…”

“…We showed previously that M. pulmonis promotes a distinct immunoblot profile of sera from technicians exposed for more than seven years to this species. Sera from humans recognized fewer proteins to M. pulmonis than sera from rats, but human sera recognized some proteins with similar molecular weight than sera from infected rats (Delgado and Timenetsky, 2001).…”

Mixed antigen ELISA of Mycoplasma pulmonis and M. arthritidis for diagnosis of murine mycoplasmosis

“…Several factors should be taken into account when analyzing the results found in the mixed ELISA. The age, lineage, sex, time of infection, and origin of the animals may interfere with the intensity of the infection and immune response to mycoplasma (Delgado & Timenetsky, 2001). This explains some results found in this study, namely, the 20% negative results in ELISA-Mp that were positive in isolation/PCR.…”

“…Since its development, the enzyme-linked immunosorbent assay (ELISA) has become the most widely used and recommended method for monitoring colonies, because of its advantages over other tests with respect to sensitivity, specificity, speed, simplicity, and cost. Therefore, its use is recommended in routine monitoring of colonies (Delgado & Timenetsky, 2001;Schoondermark-Van de Ven et al, 2006;Mähler Convenor et al, 2014) and prevalence studies (Manjunath et al, 2015). Developed by sampling, it facilitates the screening of colonies, allowing the detection of positive sera using small amounts of blood without the need to euthanize the animals.…”

Diagnóstico histopatológico e molecular da infecção por Mycoplasma sp. em ratos mantidos em biotério convencional

“…Fatores ambientais, tais como a infecção por diferentes agentes virais, em especial o vírus Sendai, frequente em biotérios brasileiros (HOWARD; STOTT;TAYLOR, 1978;SAITO et al, 1981;SCHOEB et al, 1985;HOMBERGER;THOMANN, 1994;GILIOLI et al, 1996), condições inadequadas de manejo que incluem má higiene, má qualidade do ar, excesso de amônia, podem ser fatores desencadeantes dos sinais clínicos (SCHOEB; DAVIDSON;LINDSEY, 1982;HOMBERGER;THOMANN, 1994;GILIOLI et al, 1996). Também foi relatada detecção de Mycoplama pulmonis em bioteristas expostos ao agente pela manipulação de colônias contaminadas, mas a infecção não está associada a sinais clínicos evidentes em seres humanos (DELGADO;TIMENETSKY, 2001;FERREIRA et al, 2008).…”