Experimental Model of Candida Albicans (Serotypes a and B) Adherence in Vitro

Abstract

The adherence of Candida albicans is considered an important virulence factor since it determines the colonization of the yeast in various regions of the host, being the initial cause of the infection. In this work we studied the adherence of C. albicans (serotypes A and B) to HeLa and Vero cells after culture for 18 h and 30 h in chemically defined medium (YNB) plus carbohydrate sources such as glucose, sucrose, maltose, galactose and mannose (500 mM). Each assay was performed in triplicate. The best results were obtained with the medium containing glucose. The adherence was greatest in 18 h cultures at 37ºC and at 1 h contact time. The interaction of yeast cells with the host cells was studied by scanning microscopy.

A simplified technique for evaluating the adherence of yeasts to human vaginal epithelial cells

“…The conditions of prior cultivation of the yeast interfere greatly in adherence (24). In this study, a chemically defined medium YNB, supplemented with 50 mM galactose, was used.…”

Critical factors affecting the adherence ofCandida albicansto the vaginal epithelium

“…Equal volumes of epithelial cells and yeast cells (0.25 ml) suspended in PBS adjusted at pH 7.0 were mixed and incubated on a rotator individually at 25 [28]. The cells were then ready for the application of the adherence assay.…”

“…C. albicans was grown in yeast extract broth at 25, 30, 35 and 40 • C. The yeast cells were harvested by centrifugation after 18 h (Standard growth curve was constructed to determine the timing of each of the growth phases), washed twice and resuspended in PBS (pH 7) to the desired concentration [28]. The cells were then ready for the application of the adherence assay.…”

Daya hambat xylitol dan nistation terhadap pertumbuhan Candida albicans (in vitro) (Inhibition effect of xylitol and nistatin combination on Candida albicans growth (in vitro))

“…albicans dalam rongga mulutnya dan 71,4% dalam feses 14 juga menemukan dalam media kultur yang kaya akan glukosa, sukrosa, maltosa, galaktosa meningkatkan pertumbuhan Candida dalam sel epithelial dan dental akrilik.…”