A suitable procedure to choose antimicrobials as controlling agents in fermentations performed by bacteria

Oliveira, Romulo C.; Gomez, José Gregório Cabrera; Torres, Bayardo Baptista; Netto, C L Bueno; Silva, Luiziana F. da

doi:10.1590/s1517-83822000000200004

Cited by 10 publications

(5 citation statements)

References 10 publications

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“…Polyhydroxyalkanoates (PHAs) are optically active polyesters of natural origin (15). It is synthesized in microorganisms as intracellular carbon reserve material during the excess of carbon under nitrogen limiting conditions.…”

Section: Introductionmentioning

confidence: 99%

Production and characterization of PHA from recombinant E. coli harbouring phaC1 gene of indigenous Pseudomonas sp. LDC-5 using molasses

Saranya¹,

Shenbagarathai²

2011

Braz. J. Microbiol.

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Polyhydroxyalkanoates (PHA) are biodegradable and biocompatible green thermoplastics, synthesized by wide variety of bacteria as an intracellular carbon and energy storage intermediate. They are used as an alternative to nonrenewable petroleum derived plastics. The current interest in these biopolyesters is stimulated by the search for cost-effective capitalized production. This paper attempts to achieve maximized production rate from recombinant system using inexpensive substrate. Molasses from agro-industrial waste was used to produce PHA from recombinant E.coli in batch culture. PHA yield in molasses (3.06g/L ± 0.05-75.5%) was higher than that of sucrose (2.5g/L ± 0.05 - 65.1%). Properties of the polymer produced from molasses and sucrose were analyzed by DSC, TGA, DTA, GC/MS, TLC and optical rotation studies. The findings suggested that molasses enhanced PHA production in recombinant E.coli

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Section: Introductionmentioning

confidence: 99%

Production and characterization of PHA from recombinant E. coli harbouring phaC1 gene of indigenous Pseudomonas sp. LDC-5 using molasses

Saranya¹,

Shenbagarathai²

2011

Braz. J. Microbiol.

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“…De Lima et al (1999) studied different PHA-producing bacterial strains isolated from a sugarcane agro-ecosystem for their resistance to certain antibiotics. Choosing an antimicrobial with the desirable selective toxicity is difficult and the exact amount to be applied must previously be deter-Characterization of polyhydroxyalkanoates-producing bacterial strains 651 mined since an excessive concentration may modify the global performance of the microorganism of interest or remain in the product altering its properties (Oliveira et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Screening of different contaminated environments for polyhydroxyalkanoates-producing bacterial strains

2007

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Total sixteen bacterial strains were isolated and purified from the samples collected from sugarcane molasses soil, sewage water and long-chain-hydrocarbon-contaminated area of the Punjab University, Lahore, Pakistan. Tolerance to different antibiotics was studied and strains showed multiple antibiotic resistance. All strains were characterized for Gram stain, biochemical reactions and polyhydroxyalkanoate (PHA) production. Total fourteen strains were Gram negative and two were Gram positive, while biochemically nine PHA producers showed affiliation to Pseudomonas, Enterobacter, Citrobacter, Bacillus and Escherichia. Screening for PHA production was done by Sudan black staining and nine out of sixteen strains exhibited PHA producing ability. PHA production was optimized for different growth parameters, like nitrogen concentration, pH and temperature. PHA extraction was done by solvent extraction method. Bacterial strains US1 and M1 accumulated up to 30% PHA of their cell dry weight on PHA extraction by solvent extraction method. Bacterial strain US1 was identified by 16S rRNA gene analysis as P. aeruginosa (DQ455691). PHA production was confirmed by PCR amplification of 500 bp fragment from PHA polymerase (Pha C) gene; five strains from nine PHA producers gave positive results on PCR. Pha C gene fragment of US1 was sequenced and submitted to Gene Bank under the accession number DQ455690. The amino acid sequence showed homology using the protein BLAST at 129-132 sites with different PHA synthases of the Pseudomonas sp.

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“…However, nitrofurantoin shows strong and streptomycin moderate harmful effects on A. eutrophus growth, respectively. Strongly harmful effect on PHB synthesis was only observed with nitrofurantoin (Oliveira et al, 2000). Furthermore, the successful application of vancomycin for the growth inhibition of Bacillus cereus in polyhydroxyalkanoate (PHA) production with Hydrogenophaga pseudoflava on whey containing media was also demonstrated (Koller et al, 2011).…”

Section: Introductionmentioning

confidence: 98%

“…The selection of chemicals for this purpose is a demanding process due to the fact that chemicals with desirable selective toxicity and the exact concentration to be used must be previously determined. An excessive chemical concentration may modify the performance of the working microorganism or remain in the product, altering its properties (Oliveira et al, 2000). Chemical agents usually used for this purpose are: acids, basses, organic solvents, antibiotics or oxidants.…”

Section: Introductionmentioning

confidence: 99%

Heterotrophic cultivation of Euglena gracilis on chemically pretreated media

Pavlečić

Crnić²,

Jurković³

et al. 2018

Braz. J. Chem. Eng.

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In this research, the impact of chemical agents on the growth of Euglena gracilis and contaminants (S. cerevisiae and B. subtilis) in different cultivation media was studied. E. gracilis was cultivated on modified Hutner and complex medium in Erlenmeyer flasks and a stirred tank bioreactor. H 2 O 2 and antimycin were used as suppressors of contaminant growth activities during algae cultivation. The use of antimycin as a chemical suppressor of contaminants is not recommendable because of its significant impact on the E. gracilis growth. At a H 2 O 2 concentration of 5 mg L-1 contaminant growth activities were almost completely suppressed. In these conditions, E. gracilis is capable to grow, but a further increase of H 2 O 2 concentration is related to significant reduction of algae growth. H 2 O 2 as a suppressor of contaminants has great potential for industrial application, but its optimal concentration for a particular bioprocess has to be determined in order to obtain the maximal bioprocess efficiency.

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A suitable procedure to choose antimicrobials as controlling agents in fermentations performed by bacteria

Cited by 10 publications

References 10 publications

Production and characterization of PHA from recombinant E. coli harbouring phaC1 gene of indigenous Pseudomonas sp. LDC-5 using molasses

Production and characterization of PHA from recombinant E. coli harbouring phaC1 gene of indigenous Pseudomonas sp. LDC-5 using molasses

Screening of different contaminated environments for polyhydroxyalkanoates-producing bacterial strains

Heterotrophic cultivation of Euglena gracilis on chemically pretreated media

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