Production and characterization of PHA from recombinant E. coli harbouring phaC1 gene of indigenous Pseudomonas sp. LDC-5 using molasses

Abstract

Polyhydroxyalkanoates (PHA) are biodegradable and biocompatible green thermoplastics, synthesized by wide variety of bacteria as an intracellular carbon and energy storage intermediate. They are used as an alternative to nonrenewable petroleum derived plastics. The current interest in these biopolyesters is stimulated by the search for cost-effective capitalized production. This paper attempts to achieve maximized production rate from recombinant system using inexpensive substrate. Molasses from agro-industrial waste was used to produce PHA from recombinant E.coli in batch culture. PHA yield in molasses (3.06g/L ± 0.05-75.5%) was higher than that of sucrose (2.5g/L ± 0.05 - 65.1%). Properties of the polymer produced from molasses and sucrose were analyzed by DSC, TGA, DTA, GC/MS, TLC and optical rotation studies. The findings suggested that molasses enhanced PHA production in recombinant E.coli

Improving polyhydroxyalkanoate production from inexpensive carbon sources by genetic approaches: a review

“…An engineered E.coli expressing phaC1 from Pseudomonas sp. was also found to produce PHAs from molasses: 45 higher PHA yield was achieved from molasses (75% of CDM) as compared to sucrose (65% of CDM).…”

Using biopolymer bodies for encapsulation of hydrophobic products in bacterium

“…The microscopic images were acquired by using an emission wavelength range of 570~630 nm. PHB is an opaque compound that was reported to be observable under bright field view (Anis et al, 2017;Saranya and Shenbagarathai, 2011;Song et al, 2008), so we proceeded with the confocal microscopy without staining. As expected, PHB bodies as round opaque granules were only observed in the strains that carried the PHB biosynthetic operon (E. coli PHB_1 and Co_LMVA, Fig.…”

Biotic and Abiotic Synthesis of Renewable Aliphatic Polyesters from Short Building Blocks Obtained from Biotechnology

“…[326] Sinorhizobium meliloti 41 and Hydrogenophaga pseudoflava DSM 1034 can biosynthesize P(3HB), P(3HB-co-3HV) copolyesters, and P(3HB-co-3HV-co-4HB) terpolyesters from lactose. [329][330][331] Furthermore, PHAs can also be produced directly from molasses (e.g.,s ugarcane, sugar beet,a nd soy molasses), ahigh sucrose content byproduct from the sugar industry,o ro ther sucrose-rich products (e.g.,m aple sap, sugar beet juice, pineapple juice, or oil palm frondj uice) by using C. necator, [332] A. vinelandii, [331] Enterobacter species, [333] Pseudomonas corrugate, [334] A. latus, [335] recombinant E. coli, [336,337] B. subtilis, [337] B. megaterium, [338] Bacillus cereus, [339] or mixed cultures. [327] Sucrose, ad isaccharide composed of ag lucosea nd af ructose unit, is one of the most abundant and relativelyi nexpensive carbon sources extracted from sugar-bearing raw materials, such as sugar beet and sugarcane.…”