Antinociceptive activity and toxicological study of aqueous extract of Egletes viscosa Less (Asteraceae)

Araújo, Adriano A. S.; Bonjardim, Leonardo Rigoldi; Mota, Érica M.; Albuquerque‐Júnior, Ricardo Luiz Cavalcanti de; Estevam, Charles dos Santos; Cordeiro, Luzia; Seixas, Sérgio Ricardo Stuckert; Batista, Josemar Sena; Quintans‐Júnior, Lucindo José

doi:10.1590/s1516-93322008000400018

Cited by 7 publications

(4 citation statements)

References 25 publications

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“…According to Araújo et al [14] the phytochemical screening of the aqueous extract of E. viscosa showed the presence of saponin, catechins, flavonoids, flavononols, and xanthones. The level of flavonoids was particularly high.…”

Section: Discussionmentioning

confidence: 99%

The association between drugs and herbal products: In vitro enhancement of the antibiotic activity by extracts of dry floral buttons of Egletes viscosa L. (macela)

Aquino¹,

Pereira²,

Figueredo³

et al. 2015

European Journal of Integrative Medicine

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Section: Discussionmentioning

confidence: 99%

The association between drugs and herbal products: In vitro enhancement of the antibiotic activity by extracts of dry floral buttons of Egletes viscosa L. (macela)

Aquino¹,

Pereira²,

Figueredo³

et al. 2015

European Journal of Integrative Medicine

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“…Mortality in each group within 72 hours was recorded and the animals were observed during a period of 14 days for the presence of toxicity signals. [ 15 ]…”

Section: Methodsmentioning

confidence: 99%

Antinociceptive effect of Encholirium spectabile: A Bromeliaceae from the Brazilian caatinga biome

et al. 2014

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Background:Encholirium spectabile is a species found in outcrops rocky throughout the Brazilian Caatinga.Objective:This study was carried out to evaluate the antinociceptive effects of ethanolic extract of the leaves from E. spectabile (Es-EtOH) in mice using chemical and thermal models of nociception.Material and Methods:HPLC was used to determine the fingerprint chromatogram. The Es-EtOH was examined for its antinociceptive activity at the doses of 100, 200 and 400 mg/kg intraperitoneal (i.p.). The evaluation of antinociceptive activity was carried out by the acetic acid-induced writhing, formalin and hot plate tests in mice. Rota-rod test was used for the evaluation of motor coordination.Results:In the acetic acid-induced writhing test, the Es-EtOH (100, 200 and 400 mg/kg, i.p.) reduced the number of writhings by 68.59, 79.33 and 65.28%, respectively. Additionally, Es-EtOH (100, 200 and 400 mg/kg, i.p.) decreased by 34.14, 52.61 and 60.97% the paw licking time in the first phase, as well as 89.56, 79.90 and 96.71% in the second phase of the formalin test, respectively. Es-EtOH also showed effect in the hot plate test, since increased the latency time at dose of 100 mg/kg after 60 minutes. In addition, Es-EtOH did not impair motor coordination. The presence of phenolic compounds in the extract was confirmed using HPLC. These results indicate that Es-EtOH has antinociceptive activity, probably of peripheral origin. The mechanism involved is not completely understood but, at least in part there is the participation of opioid receptors.

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“…Blood was removed by cardiac puncture for analysis of the hematological parameters red blood cell count (10 6 cells/mm 3 ), hemoglobin (g/dL), hematocrit ( %), mean corpuscular volume (MCV, μ 3 ), mean corpuscular hemoglobin (MCH, g), hemoglobin concentration, mean corpuscular (MCHC, %), leukocytes (10 3 /mm 3 ), and platelets (10 3 /mm 3 ), and were counted in a Horiba ABX Pentra 60 hematology analyzer. Blood smears were prepared and stained with panotic corant stain for determination of the differential counts of blood cells [24,25].…”

Section: Hematological and Biochemical Parametersmentioning

confidence: 99%

Acute and Subacute Toxicological Studies of Annona Vepretorum in Experimental Animals

Silva

Diniz

Lavor

et al. 2019

PMIO

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Annona vepretorum is endemic from the Brazilian Caatinga biome and is used in human nutrition. The present study aimed to investigate the toxic effects of the ethanolic extract from the leaves of this species. The leaves of A. vepretorum were collected, dried, pulverized, and macerated with ethanol to yield the crude ethanol extract of A. vepretorum. HPLC-diode array detection was used to determine the fingerprint chromatogram of the extract. In toxicity studies, the acute toxicity experimental group was administered a single dose of the ethanol extract of A. vepretorum (1 g/kg), while in the subacute toxicity experimental group, the ethanol extract of A. vepretorum was administered orally, daily for 30 days, at doses of 100 and 400 mg/kg. Death and signs of toxicity were observed and at the end, the animals were anesthetized, and blood and organs were then collected. The presence of the flavonoid rutin in the extract was confirmed using HPLC-diode array detection. In the evaluation of acute and subacute toxicity, there were no behavioral and physiological changes or signs of toxicity, and no occurrences of mice deaths were registered. The organs had normal color and preserved architecture, and no statistical variations in weight were observed. The results of the hematological and biochemical parameters after the administration of the ethanol extract of A. vepretorum showed no significant change, except in the count of the number of leukocytes and triglycerides. The histopathologic analysis of the liver, kidneys, and stomach indicated architecture with normal aspects. Thus, the toxicity study indicates low toxicity of the ethanol extract of A. vepretorum. Such information will be helpful in future clinical studies.

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Antinociceptive activity and toxicological study of aqueous extract of Egletes viscosa Less (Asteraceae)

Abstract: Egletes viscosa Less (Asteraceae), popularly known as "macela" or "macela-da-terra", is a plant widely used in popular medicine due to its anti-inflammatory, bactericidal, antidiarrhoea, mio-relaxant, antispasmodic, antinociceptive, digestive, and anti-viral

Cited by 7 publications

References 25 publications

The association between drugs and herbal products: In vitro enhancement of the antibiotic activity by extracts of dry floral buttons of Egletes viscosa L. (macela)

The association between drugs and herbal products: In vitro enhancement of the antibiotic activity by extracts of dry floral buttons of Egletes viscosa L. (macela)

Antinociceptive effect of Encholirium spectabile: A Bromeliaceae from the Brazilian caatinga biome

Acute and Subacute Toxicological Studies of Annona Vepretorum in Experimental Animals

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