Validação de metodologia espectrofotométrica para quantificação dos flavonóides de Bauhinia cheilantha (Bongard) Steudel

Sobrinho, Tadeu José da Silva Peixoto; Silva, Carlos Henrique Tabosa Pereira da; Nascimento, José William Araújo do; Monteiro, Júlio Marcelino; Albuquerque, Ulysses Paulino; Amorim, Elba Lúcia Cavalcanti de

doi:10.1590/s1516-93322008000400015

Cited by 53 publications

(19 citation statements)

References 8 publications

(10 reference statements)

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“…Moreover, differences in TFC over the months can provide parameters for the selection of the best months for harvesting this medicinal plant, and thus provide greater quality for the vegetal drug and the products commercialized from it, reducing extractivist pressure 36 . Therefore seasonal evaluation can be considered a starting point for the elaboration of new studies, aiming at enriching knowledge concerning the species, as well as adding criteria for the production and development of future phytomedicines.…”

Section: Resultsmentioning

confidence: 99%

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2014

IJPSR

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Thuja occidentalis L. is a plant widely used therapeutically. Having seen the necessity to standardize the vegetal drug and to evaluate the interference of certain environmental and seasonal factors on the synthesis of metabolites, notably flavonoids, this work considered anatomical and physical-chemical characterization assays for the aforesaid vegetal drug, in addition to phytochemical prospecting and evaluation of the flavonoid content for one year. Characterization demonstrated that a thick powder (484 µm), with loss by desiccation (6.9%), water determination (5.3%), ash content (4.5%) and volatile oils (0.7%) to be acceptable. The average content of total polysaccharides obtained for analysis was 233.2 mg/g. The anatomical evaluation pointed to structures such as spongy parenchyma, secretor channels, heteromorphic cells and ciclocitic stomata. Phytochemical prospecting showed the presence of the majority of the metabolites investigated in all the months of study. Alkaloids and coumarins were absent. The verified results for flavonoid content indicate that T. occidentalis presents a dynamic metabolism with maximum attainment of flavonoids in march/2012, with a suggestive relationship with the verified low pluviometric index. The characterization data correspond to the first parameters for quality that allow for standardization of the vegetal drug and future phytomedicines obtained from this plant.

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Section: Resultsmentioning

confidence: 99%

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2014

IJPSR

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“…The flavonoid content was determined using the colorimetric method based on the formation of the flavonoid-aluminum complex [16]. 2.0 mL aliquots of the sample (1.0 mg/mL) were mixed with 0.6 mL of glacial acetic acid, 10.0 mL of pyridine (20.0%, v/v), 2.5 mL of aluminum chloride (5.0%, w/v), and 10.9 mL of distilled water.…”

Section: Methodsmentioning

confidence: 99%

Chemical Analysis and Evaluation of Antioxidant, Antimicrobial, and Photoprotective Activities of Schinopsis brasiliensis Engl. (Anacardiaceae)

Lima-Saraiva

Oliveira

Oliveira-Júnior

et al. 2017

The Scientific World Journal

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Schinopsis brasiliensis Engl. is a native plant of Caatinga which has high concentrations of compounds capable of absorbing ultraviolet light, suggesting its potential application for the development of sunscreen preparations. After its identification and collection, this vegetable drug was submitted to a physicochemical analysis through the preparation of ethanolic extract. The phytochemical screening and analysis of extracts were carried out by high-performance liquid chromatography (HPLC) evaluation. The antioxidant activity of the extract was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) method and β-carotene bleaching test. Inhibitory hemolytic activity and morphological deformation of erythrocytes induced by H2O2 were also demonstrated and the antimicrobial activity was analyzed by the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) method. For the in vitro determination of the sun protection factor (SPF), the spectrophotometric method was used. From the analyses carried out with this species, this plant showed significant results for the antioxidant and antimicrobial activities, as well as sunscreen action. Important flavonoids were identified. These data are an important step for the development of new photoprotective cosmetic with Caatinga species, revealing importance and representing another incentive for the preservation of the species involved and analyzed in the study.

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“…Flavonoids quantification was performed based on aluminum chloride methodology with a standard quercetin (y= 0.132 x + 0.0353 R 2 = 0.9949) (Peixoto Sobrinho et al, 2008).…”

Section: Total Phenol and Flavonoids Classificationmentioning

confidence: 99%

Phytochemistry, antioxidant potential and antifungal of Byrsonima crassifolia on soil phytopathogen control

et al. 2017

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The use of chemical defensives to control fungal diseases has by consequence to impact negatively over the environment and human health, this way, the use of plant extracts with antifungal properties along with proper cultural management makes viable an alternative plant production control, specially for familiar and organic cultures. The objective of this study was to perform phytochemical and antioxidant analysis of Byrsonima crassifolia (canjiqueira) barks and evaluate its antifungal potential over Fusarium solani and Sclerotinia sclerotiorum mycelial growth. The ethanol extract from plants collected in Pantanal, Mato Grosso do Sul, Brazil was submitted to phytochemical prospection, total phenol and flavonoids quantification and antioxidant activiy determination (DPPH). To evaluate antifungal activity concentrations of 800, 1200, 1600, 2000 and 2400 µg 100 mL -1 of ethanol extract were used. Which concentration was separately incorporated in agar (PDA) and shed in Petri dishes, followed by the fungi mycelial disc where the colonies diameter was measured daily. Negatives control with agar without extract and agar with an ethanol solution were used. The B. crassifolia ethanol extract presented inhibitory activity over the fungi studied where concentrations of 800 and 1600 µg 100 mL -1 , inhibited 38% of the mycelial growth of F. solani; to S. sclerotiorum the best concentration was 2400 µg 100 mL 1 , reducing 37.5%. The antifungal bark extract potential of this specie is attributed to phenolic compounds and to triterpenes derivatives.

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Validação de metodologia espectrofotométrica para quantificação dos flavonóides de Bauhinia cheilantha (Bongard) Steudel

Cited by 53 publications

References 8 publications

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Chemical Analysis and Evaluation of Antioxidant, Antimicrobial, and Photoprotective Activities of Schinopsis brasiliensis Engl. (Anacardiaceae)

Phytochemistry, antioxidant potential and antifungal of Byrsonima crassifolia on soil phytopathogen control

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