Method for immobilizing invertase by adsorption on Dowex® anionic exchange resin

Tomotani, Ester Junko; Vítolo, Michele

doi:10.1590/s1516-93322006000200009

Cited by 5 publications

(1 citation statement)

References 5 publications

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“…Ion-exchanger resins are typical supports for ionic binding; among them are derivatives of cross-linked polysaccharides, namely, carboxymethyl- (CM-) cellulose, CM-Sepharose, diethylaminoethyl- (DEAE-) cellulose, DEAE-Sephadex, quaternary aminoethyl anion exchange- (QAE-) cellulose, QAE-dextran, QAE-Sephadex; derivatives of synthetic polymers, namely, Amberlite, Diaion, Dowex, Duolite; and resins coated with ionic polymers, namely, polyethylenimine (PEI) [ 196 ]. Recent examples include the immobilization of invertase in Dowex [ 197 ], in Duolite [ 198 ], in poly(glycidyl methacrylate-co-methyl methacrylate beads grafted with PEI [ 199 ], and in epoxy(amino) Sepabeads [ 200 ]; lactase immobilization in PEI-grafted Sepabeads [ 201 ]; fructosyltransferase in DEAE-cellulose for the production of fructosyl disaccharides [ 202 ]; glucose isomerase in DEAE-cellulose [ 203 ] or in Indion 48-R [ 204 ]; glucoamylase onto SBA-15 silica [ 205 ] and in epoxy(amino) Sepabeads [ 200 ]. Ionic binding to Sepabeads-like supports has acknowledged multipoint attachment nature.…”

Section: Immobilizationmentioning

confidence: 99%

Enzymes in Food Processing: A Condensed Overview on Strategies for Better Biocatalysts

Fernandes

2010

Enzyme Research

196

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Food and feed is possibly the area where processing anchored in biological agents has the deepest roots. Despite this, process improvement or design and implementation of novel approaches has been consistently performed, and more so in recent years, where significant advances in enzyme engineering and biocatalyst design have fastened the pace of such developments. This paper aims to provide an updated and succinct overview on the applications of enzymes in the food sector, and of progresses made, namely, within the scope of tapping for more efficient biocatalysts, through screening, structural modification, and immobilization of enzymes. Targeted improvements aim at enzymes with enhanced thermal and operational stability, improved specific activity, modification of pH-activity profiles, and increased product specificity, among others. This has been mostly achieved through protein engineering and enzyme immobilization, along with improvements in screening. The latter has been considerably improved due to the implementation of high-throughput techniques, and due to developments in protein expression and microbial cell culture. Expanding screening to relatively unexplored environments (marine, temperature extreme environments) has also contributed to the identification and development of more efficient biocatalysts. Technological aspects are considered, but economic aspects are also briefly addressed.

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Section: Immobilizationmentioning

confidence: 99%

Enzymes in Food Processing: A Condensed Overview on Strategies for Better Biocatalysts

Fernandes

2010

Enzyme Research

196

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Impacts of horseradish peroxidase immobilization onto functionalized superparamagnetic iron oxide nanoparticles as a biocatalyst for dye degradation

Keshta

Gemeay

Khamis

2021

Environ Sci Pollut Res

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Recent advances in β-galactosidase and fructosyltransferase immobilization technology

Ureta

Martins

Figueira

et al. 2020

Critical Reviews in Food Science and Nutrition

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The highly demanding conditions of industrial processes may lower the stability and affect the activity of enzymes used as biocatalysts. Enzyme immobilization emerged as an approach to promote stabilization and easy removal of enzymes for their reusability. The aim of this review is to go through the principal immobilization strategies addressed to achieve optimal industrial processes with special care on those reported for two types of enzymes: b-galactosidases and fructosyltransferases. The main methods used to immobilize these two enzymes are adsorption, entrapment, covalent coupling and cross-linking or aggregation (no support is used), all of them having pros and cons. Regarding the support, it should be cost-effective, assure the reusability and an easy recovery of the enzyme, increasing its stability and durability. The discussion provided showed that the type of enzyme, its origin, its purity, together with the type of immobilization method and the support will affect the performance during the enzymatic synthesis. Enzymes' immobilization involves interdisciplinary knowledge including enzymology, nanotechnology, molecular dynamics, cellular physiology and process design. The increasing availability of facilities has opened a variety of possibilities to define strategies to optimize the activity and re-usability of b-galactosidases and fructosyltransferases, but there is still great place for innovative developments.

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Method for immobilizing invertase by adsorption on Dowex® anionic exchange resin

Cited by 5 publications

References 5 publications

Enzymes in Food Processing: A Condensed Overview on Strategies for Better Biocatalysts

Enzymes in Food Processing: A Condensed Overview on Strategies for Better Biocatalysts

Impacts of horseradish peroxidase immobilization onto functionalized superparamagnetic iron oxide nanoparticles as a biocatalyst for dye degradation

Recent advances in β-galactosidase and fructosyltransferase immobilization technology

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