Determination of fluconazole in serum and amniotic fluid of rats by gas-chromatography/mass spectometry (GC/MS)

Abstract

Rats treated with oral dose of 100 mg/kg of fluconazole during pregnancy had their serum and amniotic fluid quantified for this drug using a GC/MS method. Fluconazole was extracted with ethyl acetate from samples and analysed by a GC-MS Shimadzu QP5050A system using a CBP-5 fused silica capillary column. Tioconazole was used as internal standard. Calibration curve was linear within the range 10.0 -300.0 μg/mL. The limit of quantification was 0.1 μg/mL and no interference was observed in the blank serum and amniotic liquid. The mean concentrations of the drug in the serum and amniotic fluid were 206. 01 ± 105.25 μg/mL and 125.34 ± 65.24 μg/mL, respectively. This procedure showed to be sensitive and efficient enough for the use in teratogenic studies of fluconazole and other azole drugs.

Salting‐out homogeneous liquid‐liquid microextraction for the determination of azole drugs in human urine: Validation using total error concept

“…A comprehensive review of these applications has been published by Ekiert et al [5]. Representative examples comprise i) HPLC with various detection mode such as UV [6], photodiode array (PDA) [7], fluorescence detector [8], MS [9] and MS/MS [10], ii) GC [11], and iii) CE [12]. Since biofluids are complicated matrices consisting of proteins, phospholipids, inorganic salts, and organic compounds, a sample clean-up step is typically required prior to the instrumental determination of the target analyte [13].…”

Determination of Fluconazole and Its Oxidation Products With Kinetic Evaluation Under Potassium Permanganate Treatment in Acidic Solutions By Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

“…Fluconazole is eliminated primarily by renal excretion, with approximately 80% of the administered dose appearing in the urine as unchanged drug (1)(2)(3). Different analytical methods, for example, spectrophotometry (4,5); capillary electrophoresis (6); micellar electrokinetic capillary chromatography (7); RP-HPLC (1,3,8), UPLC (9), or UPLC-MS/MS (10); and gas chromatography (11,12), were reported for the determination of FLU in pharmaceutical preparations and biological matrices.…”