Phytase-Producing Bacteria from Extreme Regions in Indonesia

Sajidan,; Wulandari, Rita; Sari, Evy Novita; Ratriyanto, Adı; Hailu, Hailu Weldekiros; Greiner, Ralf

doi:10.1590/s1516-89132015050173

Cited by 16 publications

(13 citation statements)

References 25 publications

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“…Group II isolate was identified as P. cineris, which produced 1.261 ± 0.111 U/mL of phytase. Bacillus amyloliquefaciens (Olajuyigbe, 2016), B. subtilis (El-Toukhy et al, 2013;Alhadi et al, 2015) and B. aryabhattai (Sajidan et al, 2015) strains have been reported as phytase producers, while ours is the first report of P. cineris as a phytase producer.…”

Section: Effects Of Culture Condition For Phytase Productionmentioning

confidence: 67%

Characterization and comparison of phytase production by Bacillus and Paenibacillus strains from Thai soils

Khianngam¹,

Pootaeng-on²,

Sonloy³

et al. 2017

MJM

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Aims: The objective of this research was to isolate, screen and identify phytase-producing bacteria from soils and a potent isolate was selected for its phytase production. Methodology and results: Eight spore-forming bacteria isolated from agricultural soils in Thailand were screened for their phytase production. They were identified as Bacillus and Paenibacillus strains based on their phenotypic characteristics and 16S rRNA gene sequence analyses. The phytase production by Bacillus amyloliquefaciens CH3-1 [Group I(a)] was 20.956 ± 0.099 U/mL, while Bacillus subtilis SR9-3 [Group I(b)] produced 20.588 ± 0.099 U/mL. Five isolates in Group I(c), identified as Bacillus aryabhattai, produced phytase at levels ranging from 2.436 ± 0.116 to 20.910 ± 0.000 U/mL, while Paenibacillus cineris CM5-3 (Group II) produced 1.261 ± 0.111 U/mL. A potent strain, CH3-1, produced the highest phytase when cultivated in Phytate Specific Medium (PSM) supplemented with 1% glucose, at pH 7.0 and incubated at 45 °C. Additionally, wheat bran and sorghum seed (0.5%) substrates were used to induce phytase production by replacing Na-phytate. Conclusion, significance and impact of study: Phytase producing bacteria were isolated from soils in Thailand. Gram-positive spore forming thermotolerant Bacillus strains displayed higher phytase activity than a Paenibacillus strain. A potent strain, CH3-1, could utilize agricultural waste as a substrate, which may be useful for animal feed supplementation.

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Section: Effects Of Culture Condition For Phytase Productionmentioning

confidence: 67%

Characterization and comparison of phytase production by Bacillus and Paenibacillus strains from Thai soils

Khianngam¹,

Pootaeng-on²,

Sonloy³

et al. 2017

MJM

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“…Isolat dari area tanah vulkanik di Selo, Jawa Tengah dan Cangkringan, Yogyakarta, Indonesia mampu menghasilkan fitase ekstraseluler yang mampu mendegradasi asam fitat (Sajidan et al, 2015). Bakteri penghasil fitase dapat diisolasi dan dikarakterisasi dari sumber air panas dari Guci, Tegal (Purwati et al, 2016).…”

Section: Pendahuluanunclassified

“…Bakteri penghasil fitase dapat disiolasi dari tanah pegunungan Himalaya, Uttarakhand, India Utara (Kumar et al, 2013). Bakteri penghasil fitase dapat diisolasi dari abu vulkanik di daerah Selo, Jawa Tengah dan Cangkringan, Boyolali (Sajidan et al, 2015).…”

Section: Hasil Dan Pembahasanunclassified

“…Koloni bakteri TG3 dan TG3-5 menunjukkan adanya aktivitas relatif yang tertinggi. Penelitian Sajidan et al (2015) menunjukkan bahwa bakteri yang diisolasi dari tanah di daerah vaulkanik Jawa Tengah mempunyai aktivitas mendegradasi asam fitat. Begitu pula bakteri yang diisolasi dari akar rizofer tanaman yang tumbuh pada tanah vulkanik di Chili (Jorquera et al, 2008;Jorquera et al, 2011).…”

Section: Gambar 3 Optimasi Temperatur Koloni Bakteri Tg3-5 (Absorbanunclassified

“…Beberapa penelitian mempunyai hasil yang berbeda dengan penelitian ini. Secara berurutan isolat bakteri D10, MC6, MS5, D16, D6 dan MC8 yang diisolasi dari tanah vulkanik di Jawa Tengah mempunyai aktivitas optimum fitase ekstraseluler pada temperatur 50, 60, 60, 50, 60 dan 40°C (Sajidan et al, 2015). Bakteri koloni TG3-5 mempunyai pH optimum pada nilai 7,0 (Gambar 4).…”

Section: Gambar 3 Optimasi Temperatur Koloni Bakteri Tg3-5 (Absorbanunclassified

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Isolasi, Seleksi Dan Optimasi Bakteri Penghasil Fitase Dari Tanah Vulkanik Di Guci, Tegal

Nuhriawangsa

Ratriyanto

Sajidan

et al. 2016

Si.Pet.

Self Cite

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The purpose of this study was to isolated, selected and characterized phytase bacteria derived from volcanic soil in the Guci, Tegal. Bacteria from the volcanic soil was isolated and selected on Luria Betany (LB) media containing 4% phytic acid. Selected colonies were optimized by physical character with determining the relative activity. Colonies of bacteria could be isolated from the volcanic soil, selected, and had the highest relative activity in colonies of TG3 and TG3-5. TG3-5 bacterial colonies producing phytate had the highest optimum value at a temperature of 55°C, pH 7, the incubation time of 90 minutes, the substrate concentration of 5% and a best metal cofactor on Zn2+ (10-4M). Volcanic soil in the Guci, Tegal containing phytase-producing bacteria with certain physical characteristics.A

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Screening and Characterization of Phytases from Bacteria Isolated from Chilean Hydrothermal Environments

et al. 2017

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Phytases are enzymes involved in organic phosphorus cycling in nature and widely used as feed additives in animal diets. Thermal tolerance is a desired property of phytases. The objectives of this study were to screen and characterize bacterial phytases from Chilean hydrothermal environments. In this study, 60% (30 of 63) of screened thermophilic (60 °C) isolates showed phytase activity in crude protein extracts. The characterization of phytase from two selected isolates (9B and 15C) revealed that both isolates produce phytases with a pH optimum at 5.0. The temperature optimum for phytate dephosphorylation was determined to be 60 and 50 °C for the phytases from the isolates 9B and 15C, respectively. Interestingly, the phytase from the isolate 15C showed a residual activity of 46% after incubation at 90 °C for 20 min. The stepwise dephosphorylation of phytate by protein extracts of the isolates 9B and 15C was verified by HLPC analysis. Finally, the isolates 9B and 15C were identified by partial sequencing of the 16S rRNA gene as members of the genera Bacillus and Geobacillus, respectively.

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Phytase-Producing Bacteria from Extreme Regions in Indonesia

Abstract: ABSTRACT, an increase in phytase activity of 10-15% was obtained.

Cited by 16 publications

References 25 publications

Characterization and comparison of phytase production by Bacillus and Paenibacillus strains from Thai soils

Characterization and comparison of phytase production by Bacillus and Paenibacillus strains from Thai soils

Isolasi, Seleksi Dan Optimasi Bakteri Penghasil Fitase Dari Tanah Vulkanik Di Guci, Tegal

Screening and Characterization of Phytases from Bacteria Isolated from Chilean Hydrothermal Environments

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