A xylanolytic bacterium, strain S1-3 T , was isolated from soil collected in Nan province, Thailand. It was characterized taxonomically based on phenotypic characteristics and 16S rRNA gene sequence comparison. The strain was a Gram-stain-positive, facultatively anaerobic, sporeforming, rod-shaped bacterium. It contained meso-diaminopimelic acid in the cell-wall peptidoglycan. The major menaquinone was MK-7. Iso-C 16 : 0 (39.5 %) and anteiso-C 15 : 0 (26.8 %) were predominant cellular fatty acids. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and lysyl-phosphatidylglycerol were the major polar lipids. The DNA G+C content was 53.3 mol%. Phylogenetic analysis using 16S rRNA gene sequences showed that strain S1-3 T was affiliated to the genus Cohnella, and was closely related to Cohnella ginsengisoli GR21-5 T and Cohnella thermotolerans CCUG 47242 T with 95.7 and 95.3 % sequence similarity, respectively. Strain S1-3 T could be clearly distinguished from related species of the genus Cohnella by its physiological and biochemical characteristics as well as by its phylogenetic position. Therefore, the strain represents a novel species of the genus Cohnella, for which the name Cohnella thailandensis sp. nov. is proposed. The type strain is S1-3 T (5KCTC 22296 T 5TISTR 1890 T 5PCU 306 T ).
A cellulose-degrading bacterium, strain FCN3-3 T , was isolated from buffalo faeces collected in Nakhonnayok province, Thailand. The strain was characterized based on its phenotypic and genotypic characteristics. Strain FCN3-3T was a Gram-positive, aerobic, spore-forming, rodshaped bacterium. It contained meso-diaminopimelic acid in cell-wall peptidoglycan. The major menaquinone was MK-7. Anteiso-C 15 : 0 (52.5 %), iso-C 16 : 0 (18.9 %) and C 16 : 0 (9.1 %) were the predominant cellular fatty acids, and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and lysyl-phosphatidylglycerol were the major phospholipids. The DNA G+C content was 58.0 mol%. Phylogenetic analysis using 16S rRNA gene sequences showed that strain FCN3-3 T was affiliated to the genus Cohnella and was closely related to Cohnella phaseoli GSPC1 T , Cohnella luojiensis HY-22R T and Cohnella hongkongensis HKU3 T , with 97.2, 96.8 and 96.3 % sequence similarity, respectively. Strain FCN3-3 T could be clearly distinguished from all known species of the genus Cohnella by its physiological and biochemical characteristics as well as its phylogenetic position and level of DNA-DNA relatedness. Therefore, the strain represents a novel species of the genus Cohnella, for which the name Cohnella cellulosilytica sp. nov. is proposed; the type strain is FCN3-3 T (5KCTC 13645 T 5TISTR 1996 T 5PCU 323 T ).
Two xylan-degrading bacteria, strains MX15-2T and MX21-2T, were isolated from soils collected in Nan province, Thailand. Cells were Gram-reaction-positive, facultatively anaerobic, spore-forming and rod-shaped. They contained meso-diaminopimelic acid in the cell-wall peptidoglycan. The major menaquinone was MK-7. iso-C16 : 0 and anteiso-C15 : 0 were the predominant cellular fatty acids. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and lysyl-phosphatidylglycerol were the major polar lipids. The genomic DNA G+C contents of strains MX15-2T and MX21-2T were 63.0 and 65.1 mol%, respectively. Phylogenetic analysis using 16S rRNA gene sequences showed that strains MX15-2T and MX21-2T were affiliated with the genus Cohnella and were closely related to Cohnella thermotolerans CCUG 47242T, with 96.5 and 95.6 % sequence similarity, respectively. The strains could be clearly distinguished from each other and from all known species of the genus Cohnella based on their physiological and biochemical characteristics as well as their phylogenetic positions and levels of DNA–DNA hybridization. Therefore, these two strains represent novel species of the genus Cohnella, for which the names Cohnella xylanilytica sp. nov. (type strain MX15-2T =KCTC 22294T =PCU 309T =TISTR 1891T) and Cohnella terrae sp. nov. (type strain MX21-2T =KCTC 22295T =PCU 310T =TISTR 1892T) are proposed.
A xylan-degrading bacterium, strain X11-1 T , was isolated from soil collected in Nan province, Thailand. The strain was characterized based on its phenotypic and genotypic characteristics. Strain X11-1 T was a Gram-stain-positive, facultatively anaerobic, spore-forming, rod-shaped bacterium. It contained meso-diaminopimelic acid in the cell-wall peptidoglycan. The major menaquinone was MK-7, anteiso-C 15 : 0 (56.6 %) and C 16 : 0 (14.0 %) were the predominant cellular fatty acids and diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine and phosphatidylglycerol were the major phospholipids. The DNA G+C content was 51.6 mol%. Phylogenetic analysis using 16S rRNA gene sequences showed that strain X11-1 T was affiliated to the genus Paenibacillus and was closely related to Paenibacillus naphthalenovorans KACC 11505 T and Paenibacillus validus CCM 3894 T , with 96.5 % sequence similarity. Therefore, the strain represents a novel species of the genus Paenibacillus, for which the name Paenibacillus xylanisolvens sp. nov. is proposed. The type strain is X11-1 T (5KCTC 13042 T 5PCU 311 T 5TISTR 1829 T ).
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