Production of Inulinase by Free and Immobilized Cells of Penicillium funiculosum p.36

Danial, Enas N.; Ayaz, Najla O.; Alnahdi, Hanan S.

doi:10.1590/s1516-8913201500167

Cited by 11 publications

(1 citation statement)

References 20 publications

(22 reference statements)

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“…Metode enzimatis memilki banyak keunggulan dibanding metode kimia karena dapat menghasilkan fruktosa murni dengan jumlah yang cukup tinggi. Hal ini sesuai didukung oleh penelitian dari Danial et al (2015), bahwa produksi enzimatik produk organik, terutama yang digunakan dalam industri makanan dan farmasi, memiliki banyak keuntungan dibandingkan proses kimia. Metode terbaik untuk menghasilkan fruktosa dalam hasil tinggi adalah melalui reaksi enzimatik (inulinase), dimana 95 % fruktosa murni dapat diproduksi setelah satu langkah dari hidrolisis enzimatik inulin.…”

Section: Aktivitas Enzim Inulinase Dari Fusan Fp4unclassified

Kemampuan Prospektif Fusan FP4 dalam Memproduksi Inulinase dan Profil Pertumbuhannya

Wijanarka

Anggraini

2018

J. Biol Papua

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Fusan FP4 is one type fused materials derived from intraspecific fusion of Pichia manshurca. The fused materials have superior capabilities compared with other materials, especially in producing inulinase enzyme (E.C. 3.2.1.7). The aims of the study are to determine the production of the enzyme inulinase of fusan FP4 and the growth profile formed. Growth can be detected by the addition of size and cell division. Growth measurements of fusan FP4 was carried out for 24 hours and observation and sampling was done every 6 hours for 24 hours. Observations of growth are conducted qualitatively by using a spectrophotometer, while the production of inulinase done using DNS method. The results of this study showed that the highest inulinase production was 1.948 U/ml and occurred at log phase with 6 hours of incubation time (t6) to 12 hours (t12). Key words: inulinase, Fusan FP4, log phase, P. manshurca.

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Section: Aktivitas Enzim Inulinase Dari Fusan Fp4unclassified

Kemampuan Prospektif Fusan FP4 dalam Memproduksi Inulinase dan Profil Pertumbuhannya

Wijanarka

Anggraini

2018

J. Biol Papua

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Fungal Inulinases: An Interesting Option for Food Sweetener Production

Flores‐Gallegos

Veana

González‐Herrera

et al. 2018

Enzymes in Food Technology

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Purification and Characterization of Exo-Inulinase from Paenibacillus sp. d9 Strain

2017

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This study intended to purify and characterise exo-inulinase of diesel-degrading Paenibacillus sp. D9. The whole genome sequencing of Paenibacillus sp. D9 revealed to possess the sacC gene that is encoded as exo-inulinase/levanase. This isolate was capable of producing a maximum of 50.9 IU/mL of exo-inulinase activity within 3 days at 30 °C, 200 rpm and pH of 7.0 on minimal salt medium agar supplemented with 1% (w/v) inulin. An exo-inulinase of 58.5 kDa was purified using ammonium sulphate precipitation, HiTrap QFF column and MMC column chromatographies with a specific activity of 4333 IU/mg, 7.1% recovery and a 4.3-fold increase in purity. The purified D9 exo-inulinase had temperature and pH optimum at 40 °C and pH 4.0, respectively, with the Michaelis constant of 5.5 mM and a maximal velocity of 476.2 IU/mg, respectively. Catalytic constant, k was calculated to be 42.6 s with a catalytic efficiency (k /K ) of 7.6 s mM. The presence of Ca enhanced the activity of D9 exo-inulinase while Hg completely inhibited the activity, other compounds such as Fe and Cu had an inhibitory effect. The results of amino acid alignment and the complete degradation of inulin into fructose by the purified enzyme confirmed that inulinase from Paenibacillus sp. D9 is an exo-form. The phylogenetic tree based on the protein sequences indicates that bacterial exo-inulinases possess a common ancestry.

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Production of Inulinase by Free and Immobilized Cells of Penicillium funiculosum p.36

Abstract: ABSTRACT

Cited by 11 publications

References 20 publications

Kemampuan Prospektif Fusan FP4 dalam Memproduksi Inulinase dan Profil Pertumbuhannya

Kemampuan Prospektif Fusan FP4 dalam Memproduksi Inulinase dan Profil Pertumbuhannya

Fungal Inulinases: An Interesting Option for Food Sweetener Production

Purification and Characterization of Exo-Inulinase from Paenibacillus sp. d9 Strain

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