Molecular characterization of arabica and Conilon coffee plants genotypes by SSR and ISSR markers

Motta, Ludymila Brandão; Soares, Taís Cristina Bastos; Ferrão, Maria Amélia Gava; Caixeta, Eveline Teixeira; Lorenzoni, Rodrigo Monte; Neto, José Dias de Souza

doi:10.1590/s1516-8913201402071

Cited by 21 publications

(16 citation statements)

References 20 publications

(16 reference statements)

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“…Robusta coffee crops were developed from introduced plants that have the highest variability in the genus Coffea (Cubry et al 2008;Motta et al 2014). Robusta coffee is a cross-pollinated species, so the resulting populations tend to be heterozygous for many gene loci.…”

Section: Introductionmentioning

confidence: 99%

Morphological variation among fifteen superior robusta coffee clones in Lampung Province, Indonesia

2018

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Ramadiana S, Hapsoro D, Yusnita Y. 2018. Morphological variation among fifteen superior robusta coffee clones inLampung Province, Indonesia. Biodiversitas 19: 1475-1481. This study aimed to characterize morphological variation among fifteensuperior robusta coffee clones in Lampung Province. The fifteen clones consisted of four clones released by the Indonesian Coffee andCocoa Research Institute (ICCRI), i.e. ‘BP 409’,‘BP936’, ‘BP939’, ‘SA 237’, together with eleven superior coffee clones selected byfarmers from Tanggamus District (‘Tugino’, ‘Wanto’, ‘Biyadi’, ‘Komari’, ‘Wardi’, ‘Wariso’) and from West Lampung District (‘TuguKuning’, ‘Tugu Hijau', ‘Tugu Biru', ‘Tugu Sari', ‘Lengkong'). Fifteen qualitative and seven quantitative morphological characters wereevaluated in a randomized complete block design with three replicates for each clone,each replication consisted of two plants. Fromeach plant, four samples were taken from four sides of the plant (north, south, west, and east). It was found that while somemorphological characters exhibited negligible variation among clones, there were significant differences between clones for othercharacters. The characters that did not differ significantly between clones were: shapes of leaf base and leaf tip; petiole color; leafvenation pattern; fruit disk shape; ripe-fruit color; and stipule shape. The characters that varied between clones were shape of leaf lamina(elliptical vs. lanceolate); fruit shape (round vs. oval); and shape of leaf margins. Morphological variation was also observed in somequantitative characters: canopy diameter; tree height; stem diameter; leaf length; leaf width; petiole length; stipule length; and number ofprimary branches.

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Section: Introductionmentioning

confidence: 99%

Morphological variation among fifteen superior robusta coffee clones in Lampung Province, Indonesia

2018

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“…In addition, selection carried out by the breeder for the best traits can lead to unconscious selection of related genotypes. Several studies have demonstrated the low genetic variability of commercially planted C. arabica cultivars (Lashermes et al 2000;Anthony et al 2001;Maluf et al 2005;Cubry et al 2008;Lashermes et al 2011;Setotaw et al 2013;Motta et al 2014;Pestana et al 2015).…”

Section: Introductionmentioning

confidence: 99%

“…Besides being locus specific, it possess several advantages, including high degree of polymorphism, repeatability, reproducibility, codominance, and multiallelism (Banerjee et al 2012;Kaur et al 2015). The advantages of molecular markers have also been highlighted in various studies on coffee plants (Diola et al 2011;Missio et al 2011;Motta et al 2014;Ferrão et al 2015;Pestana et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Molecular markers useful to discriminate Coffea arabica cultivars with high genetic similarity

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New cultivars are released every year to meet market demands. However, in species with a narrow genetic base, such as Coffea arabica, the cultivars are closely related and phenotypically similar. This hinders the accurate discrimination of genotypes using morphological descriptors in distinctness, uniformity, and stability (DUS) testing, which is required for the registration and protection of new cultivars. In this sense, molecular markers are an auxiliary tool for accurate and precise discrimination of cultivars. This study aimed to verify the informative capacity and effectiveness of a molecular marker set to discriminate among C. arabica varieties, create a database of DNA profiles and allele frequencies, analyze the genetic diversity in this collection, and explore genetic kinships. Thirty-four C. arabica cultivars/progenies, which belong to the Brazilian Cultivar Trial, were analyzed using 31 microsatellite markers. Markers with weak bands were removed, and of the remaining, 74.07% were polymorphic and revealed 47 alleles. The obtained molecular profiles revealed segregation between and within cultivars/ progenies, and genetic variability was observed between all the cultivars/progenies. Sixteen markers were selected for dendrogram construction and for fingerprinting analysis of the cultivars. The ability of these markers to detect varietal mixture and analyze diversity between and within cultivars was also discussed in detail. The results demonstrated the effectiveness of markers in distinguishing related genotypes from those with similar phenotypic traits. This biotechnological tool will assist breeders in DUS testing of cultivars.

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“…The use of molecular markers of the SSR and ISSR types in the study of diversity was efficient in carrying out the molecular characterization of coffee genotypes between and within C. arabica and C. canephora. Motta et al (2014) reported that microsatellites markers were efficient in estimating the genetic similarity and could be used to increase the efficiency in classifying the materials and selecting the candidates for parental crosses.…”

Section: Simple Sequence Repeat Ssr and Issrmentioning

confidence: 99%

Review on Genetic Diversity of Coffee (Coffea Arabica .L) in Ethiopia

2017

International Journal of Forestry and Horticulture

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Molecular characterization of arabica and Conilon coffee plants genotypes by SSR and ISSR markers

Abstract: The molecular characterization of ten genotypes of the

Cited by 21 publications

References 20 publications

Morphological variation among fifteen superior robusta coffee clones in Lampung Province, Indonesia

Morphological variation among fifteen superior robusta coffee clones in Lampung Province, Indonesia

Molecular markers useful to discriminate Coffea arabica cultivars with high genetic similarity

Review on Genetic Diversity of Coffee (Coffea Arabica .L) in Ethiopia

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