In vitro antimicrobial activity and characterization of mangrove isolates of streptomycetes effective against bacteria and fungi of nosocomial origin

Das, Arijit; Bhattacharya, Sourav; Mohammed, Abuelgasim Yegoup Hassan; Rajan, S.

doi:10.1590/s1516-89132014005000006

Cited by 23 publications

(6 citation statements)

References 17 publications

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“…To the best of our knowledge, this is the first study to evaluate the antifungal activity of Actinobacteria isolated from the Andaman Islands, against clinical pathogens of C. albicans . There are only a few reports about the actinobacteria from the Andaman islands, India which focuses on screening of enzymatic activity [ 21 ], antibacterial activity [ 22 ], and antifungal activity against multidrug resistant pathogens ( C. albicans ) [ 23 ]. Significantly, the study opens a new horizon by providing insights into the diversity and bio-potential of prominent strains isolated from mangrove ecosystem of the Andaman Islands.…”

Section: Discussionmentioning

confidence: 99%

Bioactivity Assessment of Indian Origin—Mangrove Actinobacteria against Candida albicans

et al. 2018

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Actinobacteria is found to have a potent metabolic activity against pathogens. The present study reveals the assessment of potent antifungal secondary metabolites from actinobacteria isolated from Indian marine mangrove sediments. The samples were collected from the coastal regions of Muthupet, Andaman and the Nicobar Islands. Identification was carried out using 16S rRNA analysis and biosynthetic genes (Polyketide synthase type I/II and Non-ribosomal peptide synthase) were screened. Actinobacteria were assayed for their antifungal activity against 16 clinical Candida albicans and the compound analysis was performed using gas chromatography-mass spectrometry GC-MS. The 31 actinobacterial strains were isolated and 16S rRNA gene sequencing revealed that this ecosystem is rich on actinobacteria, with Streptomyces as the predominant genus. The PCR based screening of biosynthetic genes revealed the presence of PKS-I in six strains, PKS-II in four strains and NRPS in 11 strains. The isolated actinobacteria VITGAP240 and VITGAP241 (two isolates) were found to have a potential antifungal activity against all the tested C. albicans. GC-MS results revealed that the actinobacterial compounds were belonging to heterocyclic, polyketides and peptides. Overall, the strains possess a wide spectrum of antifungal properties which affords the production of significant bioactive metabolites as potential antibiotics.

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Section: Discussionmentioning

confidence: 99%

Bioactivity Assessment of Indian Origin—Mangrove Actinobacteria against Candida albicans

et al. 2018

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“…Chủng Streptomyces I-1 ức chế đáng kể Staphylococcus aureus ATCC 25923 với 12 mm, S. aureus là 15 mm, Bacillus cereus là 17 ± 0,2 mm, Serratia marcescens là 12 mm, nấm Penicillium sp. là 12 ± 0,2 mm, nấm mem Candida albicans là 20 ± 0,5 mm và Cryptococcus neoformans là 12 mm [19]. Đặc điểm về hính thái khuẩn lạc và tế bào cho thấy chủng R10 có thể thuộc chi Bacillus.…”

Section: Hoạt Tính Kháng Khuẩn Bằng Phương Pháp Khuếch Tán Thạchunclassified

Sàng Lọc Vi Khuẩn Có Khả Năng Kháng Khuẩn Từ Rừng Ngập Mặn Ninh Ích Ninh Hòa Khánh Hòa

Mien

Khoa²

2020

VJMST

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In the context of global climate changes, mangroves are not only considered as an important ecological region but also as a source of exploitation of specific biological objects for researches and applications. In this study, mangrove-derived bacteria were isolated and screened for antimicrobial producers. Also, the strains with potential antibacterial activity were identified by 16S rRNA gene analysis. Results showed that 43% of 21 strains possessed antibacterial activity. The strain R10 showed inhibition against all four indicator bacteria and was identified as Bacillus pumilus with the highest sequence similarity of 100% to Bacillus pumilus NCTC10337 (GenBank accession number LT906438.1). The strain R7 inhibited Serratia marcescens causing severe “white spot” disease in reef building coral Acropora palmata and was closely related to Bacillus toyonesis BCT 7112 (GenBank accession number NR121761.1) with 99% sequence similarity. The strain R3 was the strongest strain against Bacillus subtilis compared with other strains which revealed the same antimicrobial activity pattern. The strain R3 was affiliated with Bacillus marisflavi TF-11 (GenBank accession number NR025240.1) with 98% sequence similarity and may be considered to represent new species. Moreover, the strain R9 inhibited only Gram negative tested strain and this strain was considered as a potential candidate for further search of antibiotics with spectrum of Gram negative bacteria.

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“…Previous studies conducted on microbial population in the South-east coast of India after the 2004 tsunami [27][28][29] had opened further arena to the quest. When giant tidal waves like tsunami occurred in the sea, it was possible that the deeper nutrient-rich water came up to the surface [30].…”

Section: Fig 3: Comparison Of Non-antagonistic and Antagonistic Isolmentioning

confidence: 99%

Antagonistic Actinobacterial Diversity of Marine Sediments Collected From Coastal Tamil Nadu

Madheslu¹,

Mani²,

Kasiviswanathan

et al. 2019

Asian J Pharm Clin Res

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Objective: The main objective of the current investigation is to identify the antagonistic potential of marine sediment actinobacterial population collected from coastal regions of Tamil Nadu and Kerala.Methods: A total of 60 marine sediment samples were collected from 27 locations of coastal Tamil Nadu and Kerala. A total of 85 morphologically distinct actinobacterial strains were isolated and taxonomically characterized. The strains were screened for its antagonistic potential against selected bacteria strains, and the obtained results were statistically analyzed. Further, the strains excelled in producing antimicrobial substances were subjected to electron microscopic and chemotaxonomic studies to get more taxonomic details.Results: The isolates were identified as different species of Streptomyces, Micromonospora, Saccharopolyspora, Actinopolyspora, Nocardia, Nocardioides, Kitasatospora, and the rest of them were categorized as “unidentified.” All the isolates were subjected to antimicrobial assay through cross-streak and well diffusion methods that endorsed 34 (40%) of them to be active against clinical and reference bacterial strains such as Staphylococcus aureus MTCC 3160, S. aureus (methicillin-resistant), Bacillus pumilus NCIM 2327, Escherichia coli MTCC 1698, E. coli, Shigella flexneri MTCC 1457, and Proteus vulgaris. The predominance of Streptomyces (18) was ascertained and followed by Saccharopolyspora (7), Micromonospora (5), Nocardia (2), and Kitasatospora (2) with considerable antimicrobial activities. Statistical analysis revealed that the isolates BDK01, BMT01, BPY03, and BRS07 showed significant antimicrobial activities. Phenotypic and chemotaxonomic analysis revealed that all four isolates are belonging under Streptomyces genus.Conclusion: The output of the present investigation revealed that the Streptomyces species are predominant in marine sediments and gain more attention for their antagonistic potential. Therefore, assessment and evaluation of marine microbial diversity were done, which could serve as a potential source for novel antibiotics.

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In vitro antimicrobial activity and characterization of mangrove isolates of streptomycetes effective against bacteria and fungi of nosocomial origin

Abstract: ABSTRACT

Cited by 23 publications

References 17 publications

Bioactivity Assessment of Indian Origin—Mangrove Actinobacteria against Candida albicans

Bioactivity Assessment of Indian Origin—Mangrove Actinobacteria against Candida albicans

Sàng Lọc Vi Khuẩn Có Khả Năng Kháng Khuẩn Từ Rừng Ngập Mặn Ninh Ích Ninh Hòa Khánh Hòa

Antagonistic Actinobacterial Diversity of Marine Sediments Collected From Coastal Tamil Nadu

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