Age-related Changes in DNA Methylation Status of hTERT Gene Promoter of Oral Epithelial Cells

Bezerra, Stephane Flaviane de Oliveira; Costa, Ludimila de Araújo; Freitas, Priscylla Alves Nascimento; Oliveira, Naila Francis Paulo de

doi:10.1590/s1516-8913201400029

Cited by 7 publications

(4 citation statements)

References 48 publications

(52 reference statements)

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“…Comparing the studies by Kamiya et al 20 and Griess et al, 12 we note that the methylated profile is common for neoplastic cells, contrary to our findings in which the methylated profile was frequent in non-tumor oral cells. It is known that the methylation profile is tissue-, site-, and age-specific 13,14,25 and to the best of our knowledge there is no other study in the literature showing the methylation profile of SOD3 in oral cells for comparison. Thus, our findings suggest that the methylated profile at the CpG −173 and −35 sites of SOD3 is a common profile for oral epithelial cells in children and adolescents.…”

Section: Discussionmentioning

confidence: 99%

TNF-α promoter hypomethylation is frequent in oncopediatric patients who recovered from mucositis

VIANA FILHO,

CASTRO COÊLHO,

QUEIROZ NETO

et al. 2024

Braz. oral res.

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The aim of this study was to investigate the DNA methylation profile in genes encoding catalase (CAT) and superoxide dismutase (SOD3) enzymes, which are involved in oxidative stress mechanisms, and in genes encoding pro-inflammatory cytokines interleukin-6 (IL6) and tumor necrosis factor-alpha (TNF-α) in the oral mucosa of oncopediatric patients treated with methotrexate (MTX ® ). This was a cross-sectional observational study and the population comprised healthy dental patients (n = 21) and those with hematological malignancies (n = 64) aged between 5 and 19 years. Oral conditions were evaluated using the Oral Assessment Guide and participants were divided into 4 groups: 1-healthy individuals; 2-oncopediatric patients without mucositis; 3-oncopediatric patients with mucositis; 4-oncopediatric patients who had recovered from mucositis. Methylation of DNA from oral mucosal cells was evaluated using the Methylation-Specific PCR technique (MSP). For CAT, the partially methylated profile was the most frequent and for SOD3 and IL6, the hypermethylated profile was the most frequent, with no differences between groups. For TNF-α, the hypomethylated profile was more frequent in the group of patients who had recovered from mucositis. It was concluded that the methylation profiles of CAT, SOD3, and IL6 are common profiles for oral cells of children and adolescents and have no association with oral mucositis or exposure to chemotherapy with MTX ® . Hypomethylation of TNF-α is associated with oral mucosal recovery in oncopediatric patients who developed oral mucositis during chemotherapy.

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Section: Discussionmentioning

confidence: 99%

TNF-α promoter hypomethylation is frequent in oncopediatric patients who recovered from mucositis

VIANA FILHO,

CASTRO COÊLHO,

QUEIROZ NETO

et al. 2024

Braz. oral res.

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“…By contrast, a different study revealed that there is no association between obesity and ADRB3 hypermethylation in the fat tissue of obese men [14], but it is not clear in that study which CpG site in the promoter was studied, and decreased ADRB3 expression was observed in this population. It is known that methylation profiles can vary in different tissues [34] and CpG sites [35], and it can also vary for populations that differ in intrinsic factors such as age or gender [36, 37] and extrinsic factors such as diet and smoking [15, 35], which may explain the difference between the studies.…”

Section: Discussionmentioning

confidence: 99%

Methylation profile of the ADRB3 gene and its association with lipid profile and nutritional status in adults

et al. 2019

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Background Defects in DNA methylation have been shown to be associated with metabolic diseases such as obesity, dyslipidemia, and hypercholesterolemia. To analyze the methylation profile of the ADRB3 gene and correlate it with lipid profile, lipid intake, and oxidative stress based on malondialdehyde (MDA) and total antioxidant capacity (TAC), homocysteine and folic acid levels, nutritional status, lifestyle, and socioeconomic variables in an adult population. A cross-sectional epidemiological study representative of the East and West regions of the municipality of João Pessoa, Paraíba state, Brazil, enrolled 265 adults of both genders. Demographic, lifestyle, and socioeconomic questionnaires and a 24-h recall questionnaire were applied by trained interviewers’ home. Nutritional and biochemical evaluation (DNA methylation, lipid profile, MDA, TAC, homocysteine and folic acid levels) was performed. Results DNA hypermethylation of the ADRB3 gene, analyzed in leukocytes, was present in 50% of subjects and was associated with a higher risk of being overweight (OR 3.28; p = 0.008) or obese (OR 3.06; p = 0.017), a higher waist–hip ratio in males (OR 1.17; p = 0.000), greater intake of trans fats (OR 1.94; p = 0.032), higher LDL (OR 2.64; p = 0.003) and triglycerides (OR 1.81; p = 0.031), and higher folic acid levels (OR 1.85; p = 0.022). Conclusions These results suggest that epigenetic changes in the ADRB3 gene locus may explain the development of obesity and non-communicable diseases associated with trans-fat intake, altered lipid profile, and elevated folic acid. Because of its persistence, DNA methylation may have an impact in adults, in association with the development of non-communicable diseases. This study is the first population-based study of the ADRB3 gene, and the data further support evaluation of ADRB3 DNA methylation as an effective biomarker.

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“…It is known that both global and site-specific DNA methylation profiles are influenced by aging. 40 , 41 Currently, global DNA hydroxymethylation is being characterised as a strong and reproducible trademark of chronological age, potentially acting on the assessment of health and disease prevention. 42 Truong et al 43 recently reported that the level of hydroxymethylation in peripheral blood T cells from humans is dependent on age, and is associated with a gradual decrease of TET3 expression levels during aging.…”

Section: Discussionmentioning

confidence: 99%

“…Although some studies show a correlation with age and gender 44 in skin tissue, it is known that these changes occur in a very specific way, that is, some genes are more susceptible than others to these factors. 29 , 32 , 40 , 44 …”

Section: Discussionmentioning

confidence: 99%

Global and gene-specific DNA methylation and hydroxymethylation in human skin exposed and not exposed to sun radiation

Silva

Melo

Costa

et al. 2017

An. Bras. Dermatol.

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Backgroundepigenomes can be influenced by environmental factors leading to the development of diseases.ObjectiveTo investigate the influence of sun exposure on global DNA methylation and hydroxymethylation status and at specific sites of the miR-9-1, miR-9-3 and MTHFR genes in skin samples of subjects with no history of skin diseases.MethodsSkin samples were obtained by punch on sun-exposed and sun-protected arm areas from 24 corpses of 16-89 years of age. Genomic DNA was extracted from skin samples that were ranked according to Fitzpatrick's criteria as light, moderate, and dark brown. Global DNA methylation and hydroxymethylation and DNA methylation analyses at specific sites were performed using ELISA and MSP, respectively.ResultsNo significant differences in global DNA methylation and hydroxymethylation levels were found among the skin areas, skin types, or age. However, gender-related differences were detected, where women showed higher methylation levels. Global DNA methylation levels were higher than hydroxymethylation levels, and the levels of these DNA modifications correlated in skin tissue. For specific sites, no differences among the areas were detected. Additional analyses showed no differences in the methylation status when age, gender, and skin type were considered; however, the methylation status of the miR-9-1 gene seems to be gender related.Study limitationsthere was no separation of dermis and epidermis and low sample size.Conclusionsun exposure does not induce changes in the DNA methylation and hydroxymethylation status or in miR-9-1, miR-9-3 and MTHFR genes for the studied skin types.

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Age-related Changes in DNA Methylation Status of hTERT Gene Promoter of Oral Epithelial Cells

Cited by 7 publications

References 48 publications

TNF-α promoter hypomethylation is frequent in oncopediatric patients who recovered from mucositis

TNF-α promoter hypomethylation is frequent in oncopediatric patients who recovered from mucositis

Methylation profile of the ADRB3 gene and its association with lipid profile and nutritional status in adults

Global and gene-specific DNA methylation and hydroxymethylation in human skin exposed and not exposed to sun radiation

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