Polymerase chain reaction (PCR) identification of terverticillate Penicillium species isolated from agricultural soils in eskişehir province

Demirel, Rasime; Sarıözlü, Nalan Yılmaz; İlhan, Semra

doi:10.1590/s1516-89132013005000004

Cited by 18 publications

(16 citation statements)

References 10 publications

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“…PCR product was separated by agarose gel electrophoresis (1% W/v in 1xTAE) and visualized by GelRed staining. PCR products were purified using EXOSAP-IT (Affimetrix) and sequenced using primers ITS1 (5'-TCCGTAGGTGAACCTGCGG-3') and ITS4 (5'-TCCTCCGCTTATTGATATGC-3') with a CEQ 8000 Genetic Analysis System (Beckman Coulter, Fullerton, CA, USA) following the method of Demirel et al (2013).…”

Section: Identificationmentioning

confidence: 99%

Antibacterial activity of endophytic fungus, Penicillium griseofulvum MPR1 isolated from medicinal plant, Mentha pulegium L.

Zerroug¹,

Sadrati²,

Demirel³

et al. 2018

Afr. J. Microbiol. Res.

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In the present study, an endophytic fungus with a considerable antimicrobial activity was isolated from the medicinal plant Mentha Pulegium L. The morphological and molecular identification revealed that this fungus is a Penicillium griseofulvum. A preliminary screening was done to choose the suitable culture medium for a maximum production of the bioactive compounds using the dual-culture agar diffusion assay. The malt extract agar (MEA) and potato dextrose agar (PDA) media were the suitable, and the MEA was selected for the further study. Extraction was done with three solvents, n-Hexane, dichloromethane and ethyl acetate, and the crude extracts were tested against Gram-positive and negative bacteria. A high activity was found with ethyl acetate and dichloromethane crude extracts against all bacteria with a maximal inhibition zones of 45.5 and 41 mm respectively obtained against Escherichia coli. The minimum inhibitory and bactericidal concentrations (MIC, MBC) of ethyl acetate crude extract were evaluated using the broth micro-dilution method. A MIC of 50 µg / ml on Gramnegative bacteria and of 100 µg / ml on Gram-positive bacteria was found. The MBC S ranged from 50 and 200 µg/ml. The time kill study has revealed a bactericidal activity of the Penicillium griseofulvum crude extract. At 24 h and for all concentrations (MIC× 2 and MIC× 4), 100% killing of the bacterial cells was achieved. These results prove that the extract of Penicillium griseofulvum can be a promising source of important bioactive molecules.

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Section: Identificationmentioning

confidence: 99%

Antibacterial activity of endophytic fungus, Penicillium griseofulvum MPR1 isolated from medicinal plant, Mentha pulegium L.

Zerroug¹,

Sadrati²,

Demirel³

et al. 2018

Afr. J. Microbiol. Res.

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“…Compared to the first sampling, the amount of bacterial contamination in pools number 1 and 2 was reduced which might be due to the increase in the level of remaining free chlorine in water. In average, the level of free chlorine and pH in the water in these pools was in an acceptable range [21][22][23][24].…”

Section: Bacteriummentioning

confidence: 99%

Assessment of Bacterial and Fungal Contamination in Public Swimming Pools in Shahrekord - IRAN

Saberianpour¹,

Momtaz²,

Ghanbari³

et al. 2016

J Trop Dis

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The current article is a descriptive-cross sectional study done to investigate the bacterial contamination in the swimming pools in the fall of 2014, in Shahrekord. 21 samples were taken from low-depth and Abstract Background and Purpose: The swimming pool is an important center of entertainment for the public. Pool area is very suitable for the transmission of various diseases, especially fungal and bacterial contamination. The aim of this study was to identify the fungus Candida Albicans, Aspergillus and Penicillium and different bacterial elements in public swimming pools in the fall of 2014. Methods: In this descriptive study, five public swimming pools were investigated in Shahrekord to examine the water contamination for fungal infection in the swimming pools during autumn of 2014. Sampling was done in two different times, and all the microbiological, chemical, and physical tests were done through standard methods. Test parameters included counting and identification of bacteria and fungi, and assessment of the remaining chlorine and pH level. Results: The results revealed that the average pH and chlorine levels were 7.8 and 1.62 mg/L, respectively. The isolated sample of bacteria included Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Staphylococcus epidermidis. Other strains of bacteria, i.e. Bacillus cereus, Enterobacter faecalis, Enterobacter aerogenes, and Clostridium perfringens were reported negative. Of 21 strains sampled from five swimming pools in Shahrekord, Penicillium species with 9.52% percent, Candida albicans with 4.76 percent, and Aspergillus species with9.5 % were isolated. Conclusions: As public swimming pools can be contaminated with different types of parasites and also fungi, observing personal and environmental hygiene, and following standards for remaining free chlorine in water can help reduce the fungal contamination. Considering the fact that most of the bacteria isolated from the pool water in the study contained Escherichia coli, we can conclude that improving the environment and hygienic behavior of the swimmers can be effective in reducing the bacterial contamination in the pools.

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“…Molecular identification: DNA were extracted from the mycelium of the isolated Penicillium digitatum and Penicillium italicum according to Demirel et al, 2013 and the Internal Transcribed Spacer (IST1) region of the ribosomal nuclear DNA (rnDNA) was amplified using PCR. Standard methods were used to get the ITS1 sequencing and was carried out at a commercial facility (Macrogen Inc., Seoul, South Korea).…”

Section: Methodsmentioning

confidence: 99%

Moringa oleifera Extraction as Alternative Option for Controlling Citrus Post-harvest Green and Blue Molds In vetro

Basahi

2019

AJAS

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The post-harvest green and blue molds caused by Penicillium digitatum and Penicillium italicum, respectively are considered universal diseases that lead to the spoilage of almost all kinds of mature citrus fruits, and cause significant losses in citrus fruits during storage and in the markets greater than what people believes. Several natural products were used to control plant pathogens as alternative of fungicides. Moringa oleifera as a natural product was used to treat several plant pathogens. Penicillium digitatum and Penicillium italicum, were isolated from mature spoiled orange (Citrus sinensis L.) and lemon (Citrus limon L.) fruits obtained from the local market of Haqle-Saudi Arabia and identified using morphological and physiological characteristics according to the key of Visagie, in addition to molecular methods based on ITS1 sequence analysis. The fungicidal activity of Moringa oleifera extract with different concentrations in controlling the growth of the isolates of Penicillium digitatum and Penicillium italicum was determined in vitro. The control activity was highly dependent on Moringa oleifera extract concentration. For instance, undiluted Moringa oleifera extract showed the highest control activity with no growth as compared to the biotic control without treatment. Diluted Moringa oleifera extract 25% and 10% reduced the fungal growth to 46.54 and 29.88%, respectively, for Penicillium digitatum and 45.83 and 27.46%, respectively, for Penicillium italicum. The results of the recent study show that Moringa oleifera extract could successfully control Penicillium digitatum and Penicillium italicum, the disease agents of green and blue molds as an environmentally friendly product.

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Polymerase chain reaction (PCR) identification of terverticillate Penicillium species isolated from agricultural soils in eskişehir province

Cited by 18 publications

References 10 publications

Antibacterial activity of endophytic fungus, Penicillium griseofulvum MPR1 isolated from medicinal plant, Mentha pulegium L.

Antibacterial activity of endophytic fungus, Penicillium griseofulvum MPR1 isolated from medicinal plant, Mentha pulegium L.

Assessment of Bacterial and Fungal Contamination in Public Swimming Pools in Shahrekord - IRAN

Moringa oleifera Extraction as Alternative Option for Controlling Citrus Post-harvest Green and Blue Molds In vetro

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