Separation of polyhydroxyalkanoates-producing bacterial strains using PHA synthase gene and their evaluation for PHA deposition

Khan, Abdul Basit; Khattak, Mehmood Iqbal; Tarar, Omer Mukhtar; Habib, Farzana; Jamil, Khalid; Yasmin, Arfa; Parvez, Shoukat

doi:10.1590/s1516-89132013000400015

Cited by 5 publications

(3 citation statements)

References 15 publications

(14 reference statements)

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“…If the PHA content is high in the cells, the FTIR spectra of PHA can also differentiate the various structures of PHB (scl), mcl-PHAs and PHA consisting of HB and mcl-3HA monomers (80). Genotypic-based screening method is specific, reliable and quick which circumvents the major drawbacks inherent in phenotypic detection methods (78,(81)(82)(83)(84). For genotypic-based screening, the degenerate or and specific oligo nucleotide primers are designed based on multiple sequence alignment results and are used as PCR primers to detect PHA synthase genes (78,79,81,(83)(84)(85).…”

Section: Screening Of Bacteria For Pha Productionmentioning

confidence: 99%

Bacterial polyhydroxyalkanoates-eco-friendly next generation plastic: Production, biocompatibility, biodegradation, physical properties and applications

Muhammadi

Shabina

Afzal

et al. 2015

Green Chemistry Letters and Reviews

257

139

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Polyhydroxyalkanoates (PHAs) are intracellular aliphatic polyesters synthesized as energy reserves, in the form of water-insoluble, nano-sized discrete and optically dense granules in cytoplasm by a diverse bacteria and some archae under conditions of limiting nutrients in the presence of excess carbon source. Bacteria synthesize different PHAs from coenzyme A thioesters of respective hydroxyalkanoic acid, and degrade intracellularly for reuse and extracellularly in natural environments by other microorganisms. In vivo, PHAs exist as amorphous mobile liquid and water-insoluble inclusions but in vitro, exhibit material and mechanical properties, ranging from stiff and brittle crystalline to elastomeric and molding, similar to petrochemical thermoplastics. Further, they are hydrophobic, isotactic, biocompatible and exhibit piezoelectric properties. But as commodity plastics their applications are limited by high production cost, low yield, in vivo degradation, complexity of technology and difficulty of extraction. Therefore, to replace the conventional plastic with PHAs, it is prerequisite to standardize the PHA production systems.

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Section: Screening Of Bacteria For Pha Productionmentioning

confidence: 99%

Bacterial polyhydroxyalkanoates-eco-friendly next generation plastic: Production, biocompatibility, biodegradation, physical properties and applications

Muhammadi

Shabina

Afzal

et al. 2015

Green Chemistry Letters and Reviews

257

139

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“…Among the PHA-synthesising gene clusters, the PHA synthase ( phaC ) gene is key to the catalysis of PHA formation from hydroxyacyl-CoA thioesters. Moreover, PhaC influences the final polymer composition as well as substrate specificity [ 10 ]. Yet, due to the uncultivability of most marine bacteria, only a limited fraction of PhaC enzymes have been identified, characterised, and expressed for these organisms [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Polyhydroxyalkanoate (PHA) Biopolymer Synthesis by Marine Bacteria of the Malaysian Coral Triangle Region and Mining for PHA Synthase Genes

et al. 2022

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Polyhydroxyalkanoate (PHA), a biodegradable and plastic-like biopolymer, has been receiving research and industrial attention due to severe plastic pollution, resource depletion, and global waste issues. This has spurred the isolation and characterisation of novel PHA-producing strains through cultivation and non-cultivation approaches, with a particular interest in genes encoding PHA synthesis pathways. Since sea sponges and sediment are marine benthic habitats known to be rich in microbial diversity, sponge tissues (Xestospongia muta and Aaptos aaptos) and sediment samples were collected in this study from Redang and Bidong islands located in the Malaysian Coral Triangle region. PHA synthase (phaC) genes were identified from sediment-associated bacterial strains using a cultivation approach and from sponge-associated bacterial metagenomes using a non-cultivation approach. In addition, phylogenetic diversity profiling was performed for the sponge-associated bacterial community using 16S ribosomal ribonucleic acid (16S rRNA) amplicon sequencing to screen for the potential presence of PHA-producer taxa. A total of three phaC genes from the bacterial metagenome of Aaptos and three phaC genes from sediment isolates (Sphingobacterium mizutaii UMTKB-6, Alcaligenes faecalis UMTKB-7, Acinetobacter calcoaceticus UMTKB-8) were identified. Produced PHA polymers were shown to be composed of 5C to nC monomers, with previously unreported PHA-producing ability of the S. mizutaii strain, as well as a 3-hydroxyvalerate-synthesising ability without precursor addition by the A. calcoaceticus strain.

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“…In an attempt to reduce production costs in the recent years, focus has shifted to the use of less valuable, renewable materials as substrates for PHA production, as soy molasses (Solaiman et al, 2006), wheat-based co-products (Koutinas et al, 2007), refined and crude glycerol (Cavalheiro et al, 2009;Zhu et al, 2010;Ashby et al, 2011). The isolation and the purification of bacterial PHAs are the key step of the process profitability in the fermentation system (Khan et al, 2013). Detailed studies have been carried out to investigate that which fermentation system i.e.…”

Section: Introductionmentioning

confidence: 99%

UTILIZATION OF MUSTARD OIL FOR THE PRODUCTION OF POLYHYDROXYALKANOATES BY Pseudomonas aeruginosa

Javed¹,

Jamil²

2015

J microb biotech food sci

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With the unnecessary use of plastics and cumulative pressure being placed on capacities available for plastic waste disposal, the need for biodegradable plastics and biodegradation of plastic wastes has assumed increasing importance in the last few years. Bioplastic production from mustard oil was considered relatively cheap, easily available, included in vegetable oil and don’t having much volatile characteristics. Total of 67 bacterial strains were isolated and purified from different regions of the Pakistan, and were checked for Polyhydroxyalkanoates (PHA) production by Sudan black and Nile blue staining. Quantitative analysis for biodegradable plastic produced by different bacterial species was performed by Modified surfactant hypochlorite method. High PHA production was detected in 35 strains belonging to different genera including Pseudomonas, Staphylococcus, Escherichia and Enterobacter. Fermentation and PHA production was done in batch culture. The PHA production of P. aeruginosa by mustered oil cultivation was studied under six experimental conditions, such as air flow rates, pH, Temperature, optical density, substrates concentration and cell dry weight. PHA production of Pseudomonas species were subsequently authenticated at molecular level by PCR amplifications and sequence analysis. PHA polymerase 1 (PhaC1) and PHA polymerase 2 (PhaC2) from Pseudomonas aeruginosa were amplified, sequenced and submitted to gene bank.

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Separation of polyhydroxyalkanoates-producing bacterial strains using PHA synthase gene and their evaluation for PHA deposition

Abstract: ABSTRACT

Cited by 5 publications

References 15 publications

Bacterial polyhydroxyalkanoates-eco-friendly next generation plastic: Production, biocompatibility, biodegradation, physical properties and applications

Bacterial polyhydroxyalkanoates-eco-friendly next generation plastic: Production, biocompatibility, biodegradation, physical properties and applications

Polyhydroxyalkanoate (PHA) Biopolymer Synthesis by Marine Bacteria of the Malaysian Coral Triangle Region and Mining for PHA Synthase Genes

UTILIZATION OF MUSTARD OIL FOR THE PRODUCTION OF POLYHYDROXYALKANOATES BY Pseudomonas aeruginosa

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