UTILIZATION OF MUSTARD OIL FOR THE PRODUCTION OF POLYHYDROXYALKANOATES BY Pseudomonas aeruginosa

Abstract

With the unnecessary use of plastics and cumulative pressure being placed on capacities available for plastic waste disposal, the need for biodegradable plastics and biodegradation of plastic wastes has assumed increasing importance in the last few years. Bioplastic production from mustard oil was considered relatively cheap, easily available, included in vegetable oil and don’t having much volatile characteristics. Total of 67 bacterial strains were isolated and purified from different regions of the Pakistan, and were checked for Polyhydroxyalkanoates (PHA) production by Sudan black and Nile blue staining. Quantitative analysis for biodegradable plastic produced by different bacterial species was performed by Modified surfactant hypochlorite method. High PHA production was detected in 35 strains belonging to different genera including Pseudomonas, Staphylococcus, Escherichia and Enterobacter. Fermentation and PHA production was done in batch culture. The PHA production of P. aeruginosa by mustered oil cultivation was studied under six experimental conditions, such as air flow rates, pH, Temperature, optical density, substrates concentration and cell dry weight. PHA production of Pseudomonas species were subsequently authenticated at molecular level by PCR amplifications and sequence analysis. PHA polymerase 1 (PhaC1) and PHA polymerase 2 (PhaC2) from Pseudomonas aeruginosa were amplified, sequenced and submitted to gene bank.

PHA-Based Bioplastic: a Potential Alternative to Address Microplastic Pollution

“…PHAs granules are used by many bacterial species as a source of carbon stores and energy in their cells (Thiruchelvi et al, 2020 ). The microorganisms such as Bacillus cereus (Narayanan et al, 2020a ), Cupriavidus necator (Aramvash et al, 2015 ), Pseudomonas aeruginosa (Javed & Jamil, 2015 ), Azospirillum rubrum (Jin & Nikolau, 2014 ), Brevundimonas spp . , and Enterococcus spp .…”

“…NAP11 Cardboard industry waste water – 5.236 79.27 Bacillus sp. NA10 Cardboard industry effluent 7.8 5.202 66.70 Bhuwal et al ( 2014 ) Bacillus thuringiensis IAM 12,077 Starch 3.6 2.6 72.3 Gowda and Shivakumar ( 2014 ) Cupriavidus necator Fructose 11.6 7.48 64.48 Aramvash et al ( 2015 ) Pseudomonas aeruginosa Mustard oil – 9.01 20.1 Javed and Jamil ( 2015 ) Bacillus sp. Sugarcane bagasse 9.0 5.0 55.55 Getachew and Woldesenbet ( 2016 ) Pseudomonas putida Waste frying oil 4.90 2.80 57 Gatea et al ( 2018 ) Bacillus siamensis PD-A10 Orange peel 2.66 2.16 81.2 Vijay and Tarika ( 2019 ) Bacillus subtilis JCM 1465 Onion peel 3.33 2.93 88 Staphylococcus aureus JH1 …”