Mesenchymal stem cells: emphasis in adipose tissue

Abstract

ABSTRACT

Estudios en neurociencias: aportes para la investigación en cultivo de células madre mesenquimales

“…Tales características, intrínsecas a todas las células mesenquimales (CM), las hacen muy atractivas para su uso en terapia celular y en medicina regenerativa. Originalmente se creía que estas células se encontraban exclusivamente en la médula ósea, donde residen los progenitores y precursores hematopoyéticos junto con otras células que proveen un soporte estructural para la hematopoyesis, como el estroma de la médula ósea; sin embargo, también se ha demostrado la presencia de estas células en el tejido graso (Zuk, Zhu y Ashjian, 2002;Zuk, 2010;Rastegar, Shenaq, Huang, 2010;Bertassoli et al, 2013;De Girolamo et al, 2013), páncreas, hígado, músculo esquelético, dermis, membrana sinovial, hueso trabecular, sangre del cordón umbilical, tejido pulmonar, pulpa dental y ligamento periodontal (Macías-Abraham, Del Valle Pérez, Hernández-Ramírez y Ballester-Santovenia, 2010). Con respecto a la cantidad de MSC, en médula ósea constituyen un aproximado de 0.0001 % -0.01 % de todas las células nucleadas de la médula ósea, a diferencia del tejido adiposo que contiene aproximadamente 100 000 MSC por gramo de grasa, lo que constituye para el tejido adiposo una fuente abundante y de fácil obtención de MSC, para ingeniería de tejidos y aplicaciones en medicina regenerativa (Ra et al, 2011).…”

Rat dental pulp stem cells: isolation and phenotypic characterization method aiming bone tissue bioengineering

“…The association of chitosan and embryonic stem cells (Weng-Ning et al 2009); PGA-PLLA and umbilical cord stem cells (Wu et al 2004); fibrin scaffold and mesenchymalstem cells (Bensaid et al 2003); poly (D,L-lactic-co-glycolic acid) hydroxyapatite and embryonic stem cells (Kim et al 2008); phosphate ceramic biphasic calcium and mesenchymal stem cells (Arinzeh et al 2007); are all examples of stem cells seeded onto biomaterials for tissue reconstruction. Mesenchymal stem cells (MSC) have been indicated as the first choice in tissue bioengineering studies, by presenting advantages as high differentiation potential, immunosuppressed effects, and viability after expansion in culture (Bensaid et al 2003;Li et al 2005;Uematsu et al 2005;Arinzeh et al 2007;Lyra et al 2007;Bertassoli et al 2013). MSC were originally isolated from bone marrow (Arinzeh et al 2007).…”

“…MSC were originally isolated from bone marrow (Arinzeh et al 2007). After that, these cells were found in various organs and tissues, including in the dental pulp (Bensaid et al 2003;Li et al 2005;Uematsu et al 2005;Arinzehet al 2007;Lyra et al 2007;Bertassoli et al 2013). Gronthos and co-workers (2000) published the first report about the isolation and identification of an adult dental pulp progenitor population.…”

Storage effect on viability and biofunctionality of human adipose tissue-derived stromal cells

“…The superficial adipose tissue and the deep subcutaneous fat constitute the largest ASCs stores, the latter accounting for approximately 80% of the total body fat. This unique reservoir of ASCs is advantageous because of its accessibility, large quantities, minimally invasive cell isolation procedures [1,5,12] and relatively lenient ethics and safety regulations. Therefore, the isolated ASCs present an attractive tool for curing a broad spectrum of disorders, injuries and diseases [4,5,13,14], including bone reconstruction [15,16].…”