Rat dental pulp stem cells: isolation and phenotypic characterization method aiming bone tissue bioengineering

Bertassoli, Bruno Machado; Costa, Emanuela Silva; Sousa, Cristiane Aparecida; Albergaria, Juliano Douglas Silva; Maltos, K. L. M.; Góes, Alfredo M.; Matins, Thais Maria da Mata; Silva, Gerluza Aparecida Borges; Jorge, Érika Cristina

doi:10.1590/1678-4324-2016150613

Cited by 7 publications

(5 citation statements)

References 50 publications

(40 reference statements)

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“…After sacrifice, the incisors from animals were carefully separated from the jawbones. Dental pulps were gently isolated using a sterile dental explorer and the apical third of the tooth was cut off to obtain the apical epithelial buds as previously described (27). The remaining pulp tissue was minced into small pieces and treated with solution containing 3 mg/mL collagenase type 1 (Sigma-Aldrich, St. Louis, MO, USA) and 4 mg/mL dispase (Sigma-Aldrich) for 60 min at 37°C.…”

Section: Methodsmentioning

confidence: 99%

Resolvin D2 Induces Resolution of Periapical Inflammation and Promotes Healing of Periapical Lesions in Rat Periapical Periodontitis

et al. 2019

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Periapical periodontitis results from pulpal infection leading to pulpal necrosis and resorption of periapical bone. The current treatment is root canal therapy, which attempts to eliminate infection and necrotic tissue. But, in some cases periapical inflammation doesn't resolve even after treatment. Resolvins belongs to a large family of specialized pro-resolving lipid mediators that actively resolves inflammation signaling via specific receptors. Resolvin D2 (RvD2), a metabolite of docosahexaenoic acid (DHA), was tested as an intracanal medicament in rats in vivo . Mechanism was evaluated in rat primary dental pulp cells (DPCs) in vitro . The results demonstrate that RvD2 reduces inflammatory cell infiltrate, periapical lesion size, and fosters pulp like tissue regeneration and healing of periapical lesion. RvD2 enhanced expression of its receptor, GPR18, dentin matrix acidic phosphoprotein 1 (DMP1) and mineralization in vivo and in vitro . Moreover, RvD2 induces phosphorylation of Stat3 transcription factor in dental pulp cells. We conclude that intracanal treatment with RvD2 resolves inflammation and promoting calcification around root apex and healing of periapical bone lesions. The data suggest that RvD2 induces active resolution of inflammation with pulp-like tissue regeneration after root canal infection and thus maybe suitable for treating periapical lesions.

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Section: Methodsmentioning

confidence: 99%

Resolvin D2 Induces Resolution of Periapical Inflammation and Promotes Healing of Periapical Lesions in Rat Periapical Periodontitis

et al. 2019

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“…DPSCs were isolated from lower incisors of Sprague Dawley rats as previously described 10 (see Supplementary Data for details).…”

Section: Cell Isolation and Culturementioning

confidence: 99%

“…9 ), and great plasticity and regenerative capacity. 10 DPSCs are similar to BM-MSCs in their ability to repair musculoskeletal tissues (reviewed in Ref. 8 ), good compatibility with biomaterials, 11 and their demonstrated ability to promote bone formation in mandibular defects.…”

Section: Introductionmentioning

confidence: 99%

Electrical Stimulation Decreases Dental Pulp Stem Cell Osteo-/Odontogenic Differentiation

Oliveira

Leppik

Keswani

et al. 2020

BioResearch Open Access

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Dental pulp stem cells (DPSCs) have great potential for use in tissue engineering (TE)-based dental treatments. Electrical stimulation (EStim) has been shown to influence cellular functions that could play an important role in the success of TE treatments. Despite many recent studies focused on DPSCs, few have investigated the effect EStim has on these cells. The aim of this research was to investigate the effects of direct current (DC) EStim on osteo-/odontogenic differentiation of DPSCs. To do so cells were isolated from male Sprague Dawley rats (7-8 weeks old), and phenotype characterization and multilineage differentiation analysis were conducted to verify their ''stemness.'' Different voltages of DC EStim were administrated 1 h/day for 7 days, and the effect of EStim on DPSC osteo-/odontogenic differentiation was assessed by measuring calcium and collagen deposition, alkaline phosphatase (ALP) activity, and expression of osteo-and odontogenic marker genes at days 7 and 14 of culture. We found that while 10 and 50 mV/mm of EStim had no effect on cell number or metabolic activity, 100 mV/mm caused a significant reduction in cell number, and 150 mV/mm resulted in cell death. Despite increased gene expression of osteo-/odontogenic gene markers, Osteocalcin, RunX2, BSP, and DMP1, at day 7 in EStim treated cells, 50 mV/mm of EStim decreased collagen deposition and ALP activity at both time points, and calcium deposition was found to be lower at day 14. In conclusion, under the conditions tested, EStim appears to impair DPSC osteo-/odontogenic differentiation. Additional studies are needed to further characterize and understand the mechanisms involved in DPSC response to EStim, with an eye toward its potential use in TEbased dental treatments.

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“…The presence or absence of these markers form the criteria for identifying stem cells. The isolated cells presented a high expression of CD90 and CD44 (the well-known marker of dental pulp stem cells [ 9 , 35 ]) and low expression of CD34 (a primitive hematopoietic progenitor and endothelial cell marker) ( Figure 3 c), suggesting that the prepared rDPCs are likely heterogeneous cells, including dental pulp stem cells.…”

Section: Resultsmentioning

confidence: 99%

Tailoring Silicon Nitride Surface Chemistry for Facilitating Odontogenic Differentiation of Rat Dental Pulp Cells

Gong

Honda

Adachi

et al. 2021

IJMS

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Silicon nitride (Si3N4) can facilitate bone formation; hence, it is used as a biomaterial in orthopedics. Nevertheless, its usability for dentistry is unexplored. The aim of the present study was to investigate the effect of Si3N4 granules for the proliferation and odontogenic differentiation of rat dental pulp cells (rDPCs). Four different types of Si3N4 granules were prepared, which underwent different treatments to form pristine as-synthesized Si3N4, chemically treated Si3N4, thermally treated Si3N4, and Si3N4 sintered with 3 wt.% yttrium oxide (Y2O3). rDPCs were cultured on or around the Si3N4 granular beds. Compared with the other three types of Si3N4 granules, the sintered Si3N4 granules significantly promoted cellular attachment, upregulated the expression of odontogenic marker genes (Dentin Matrix Acidic Phosphoprotein 1 and Dentin Sialophosphoprotein) in the early phase, and enhanced the formation of mineralization nodules. Furthermore, the water contact angle of sintered Si3N4 was also greatly increased to 40°. These results suggest that the sintering process for Si3N4 with Y2O3 positively altered the surface properties of pristine as-synthesized Si3N4 granules, thereby facilitating the odontogenic differentiation of rDPCs. Thus, the introduction of a sintering treatment for Si3N4 granules is likely to facilitate their use in the clinical application of dentistry.

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Rat dental pulp stem cells: isolation and phenotypic characterization method aiming bone tissue bioengineering

Abstract: Dental pulp stem cells (DPSC)

Cited by 7 publications

References 50 publications

Resolvin D2 Induces Resolution of Periapical Inflammation and Promotes Healing of Periapical Lesions in Rat Periapical Periodontitis

Resolvin D2 Induces Resolution of Periapical Inflammation and Promotes Healing of Periapical Lesions in Rat Periapical Periodontitis

Electrical Stimulation Decreases Dental Pulp Stem Cell Osteo-/Odontogenic Differentiation

Tailoring Silicon Nitride Surface Chemistry for Facilitating Odontogenic Differentiation of Rat Dental Pulp Cells

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