Organogenesis from in vitro-derived leaf and internode explants of Hoya wightii ssp. palniensis -a vulnerable species of Western Ghats

Lakshmi, S.; Benjamin, J. H. Franklin; Kumar, T. Senthil; Murthy, G. V. S.; Rao, M. S.

doi:10.1590/s1516-89132013000300010

Cited by 10 publications

(5 citation statements)

References 24 publications

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“…The positive effect of BAP and Kinetin has been reported in organogenic mode of shoot formation and elongation (Emmanuel et al 2000). The synergistic effect of cytokine and gibberellins in promoting the conversion of embryos to plantlets have been reported in a number of other species (Zlenko et al 2002;Martin 2004;Paramageetham et al 2004;Cangahuala Inocente et al 2007;Junaid et al 2007;Lakshmi et al 2013). …”

Section: Resultsmentioning

confidence: 72%

High efficient somatic embryogenesis development from leaf cultures of Citrullus colocynthis (L.) Schrad for generating true type clones

Ramakrishna

Shasthree

2016

Physiol Mol Biol Plants

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We report an efficient somatic embryogenesis and plant regeneration system using leaf cultures of Citrullus colocynthis (L.) and assessed the effect of plant growth regulators on the regeneration process. Initially leaf explants were cultured on Murashige and Skoog medium supplemented with different concentrations of auxins viz., 2,4-dichlorophenoxyacetic acid, 1-naphthaleneacetic acid, gibberellic acid alone and along with combination of 6-benzylaminopurine. The different forms of calli such as compact, white friable, creamy friable, brownish nodular, green globular and green calli were induced from the leaf explants on MS medium containing different concentrations of auxins and gibberellins. Subsequently initial callus was subcultured at 1.5 mg L -1 BAP ? 1.0 mg L -1 2,4-D which resulted in 25 % somatic embryos from 85 % nodular embryogenic nodular callus that is highest percentage. Similarly the lowest percentage of somatic embryos was recorded at 2.5 mg L -1 BAP ? 0.5 mg L -1 NAA from 55 % embryogenic globular callus i.e., 16 %. High frequency of embryo development takes place at intermittent light when compared with continuous light in the individual subcultures. The cotyledonary embryos were developed into complete platelets on MS medium. In vitro regenerated plantlets were washed to remove the traces of agar and then transferred to sterile vermiculite and sand (2:1) containing pot.

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Section: Resultsmentioning

confidence: 72%

High efficient somatic embryogenesis development from leaf cultures of Citrullus colocynthis (L.) Schrad for generating true type clones

Ramakrishna

Shasthree

2016

Physiol Mol Biol Plants

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“…Synergistic effect of cytokinin and gibberellin in promoting the conversion of embryos to plantlets has also been reported in a number of species (Zlenko et al 2002, Martin 2004, Paramageetham et al 2004, Cangahuala-Inocente et al 2007, Junaid et al 2007. GA 3 also have been used in the elongation of regenerated shoots (Lakshmi et al 2013). The effectiveness of GA 3 could be due to gene activation or synthesis of new gene-products for the completion of embryo development.…”

Section: Discussionmentioning

confidence: 88%

Somatic embryogenesis and plant regeneration in Wedelia calendulacea Less. an endangered medicinal plant

Sharmin

Alam

Sheikh

et al. 2014

Braz. arch. biol. technol.

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In this work, plant regeneration via somatic embryogenesis was achieved from leaf and internode derived callus of Wedelia calendulacea, an endangered medicinal plant. Primary callus was induced by culturing leaf disc and internode explant on Murashige and Skoog medium supplemented with 2.0 mg L-1 of 2,4-D under light condition. Transfer of embryogenic callus on a reduced concentration of 2,4-D facilitated somatic embryo development while calluses remained unorganized at the same 2,4-D level. A histological analysis confirmed somatic embryo by revealing the presence of a closed vascular system in the developing embryos and lack of a vascular connection with surrounding callus tissues. Somatic embryos germinated into plantlets upon transfer on MS medium containing 1.0 mg L-1 BAP plus 0.5 mg L-1 GA 3. Plantlets were acclimatized successfully and survived under soil condition. This is the first on somatic embryogenesis of W. calendulacea. This result could facilitate genetic transformation of this important medicinal plant.

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“…Relatively mature and more vascular internodes' tissue than leaf might be responsible for the higher shoots regeneration (Dhital et al, 2011). Moreover, internodes might hold ample cytokinin during the incision to develop adventitious shoot at the time of explant inoculation in medium (Lakshmi et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Direct in vitro organogenesis from leaf and internode of Coccinia cordifolia (L.) Cogn.

Sarkar¹,

Rahman

Alam

2022

HBR

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The current study was carried out to elucidate a reproducible protocol to develop plants directly from leaf and internodes to facilitate the genetic transformation in Coccinia cordifolia (L.) Cogn., a medicinal plant of the cucurbitaceae family. In vitro grown leaf and internodes were used, which were collected for regenerated shoots from field-grown nodal segments that were sterilized by 0.1% HgCl2 treatment for 6 minutes. The nodal segment cultured on BAP, Kn and BAP, NAA combination where 1.5 mg/L BAP solitary supplement and augmented supplement with 0.1 mg/L NAA was most effective as 80% shoots regenerated with 4.0±0.37 and 2.7±0.45 shoots per culture, respectively. Collected leaf and internode responded 90% at 1.5 mg/L BAP + 0.1 mg/L NAA fortified full-strength MS medium. The highest number of shoots also regenerated in the same combination which were 8.1 ± 0.30 and 10.2±0.40, respectively and found internode as the best explant for direct organogenesis. For root induction, half strength of MS medium supplemented with 0.1 mg/L IBA was found most effective. The highest number of roots regenerated per shoot (6.8±0.10) and root length (2.8±0.20). The successful acclimatization of the in vitro (80%) grown plantlets proved the validity of the developed protocol of using biotechnological techniques for improving the plant.

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Organogenesis from in vitro-derived leaf and internode explants of Hoya wightii ssp. palniensis -a vulnerable species of Western Ghats

Abstract: ABSTRACT

Cited by 10 publications

References 24 publications

High efficient somatic embryogenesis development from leaf cultures of Citrullus colocynthis (L.) Schrad for generating true type clones

High efficient somatic embryogenesis development from leaf cultures of Citrullus colocynthis (L.) Schrad for generating true type clones

Somatic embryogenesis and plant regeneration in Wedelia calendulacea Less. an endangered medicinal plant

Direct in vitro organogenesis from leaf and internode of Coccinia cordifolia (L.) Cogn.

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