Somatic embryogenesis and plant regeneration in Wedelia calendulacea Less. an endangered medicinal plant

Abstract

In this work, plant regeneration via somatic embryogenesis was achieved from leaf and internode derived callus of Wedelia calendulacea, an endangered medicinal plant. Primary callus was induced by culturing leaf disc and internode explant on Murashige and Skoog medium supplemented with 2.0 mg L-1 of 2,4-D under light condition. Transfer of embryogenic callus on a reduced concentration of 2,4-D facilitated somatic embryo development while calluses remained unorganized at the same 2,4-D level. A histological analysis confirmed somatic embryo by revealing the presence of a closed vascular system in the developing embryos and lack of a vascular connection with surrounding callus tissues. Somatic embryos germinated into plantlets upon transfer on MS medium containing 1.0 mg L-1 BAP plus 0.5 mg L-1 GA 3. Plantlets were acclimatized successfully and survived under soil condition. This is the first on somatic embryogenesis of W. calendulacea. This result could facilitate genetic transformation of this important medicinal plant.

An efficient regeneration system through in vitro somatic embryogenesis of barley (Hordeum vulgare L.)

“…Sometimes it is very difficult to culture and regenerate agronomically important crops (Puhan and Siddiq 2013). Somatic embryogenesis is a multi-step regeneration process starting with formation of pro-embryogenic masses, followed by somatic embryo formation, maturation and plant regeneration (Arnold et al 2002, Sharmin et al 2014. The difference in the composition of culture medium and the concentrations of hormones affect the callus induction and regeneration ability of barley and other plant genotypes (Tariq et al 2008, Haque and.…”

Multiplicación in vitro y embriogénesis somática de Perezia pinnatifida (Asteraceae) planta medicinal andina

“…2). Por otro lado, las auxinas también intervienen en la formación de raíces (Sharmin et al 2014) lo cual explicaría la formación de callos y raíces en el tratamiento con ANA 2.0 mg.L -1 (Fig. 3).…”

“…Por lo tanto, los callos embriogénicos fueron transferidos a medios con BAP solo o en combinación con ANA (Fig. 5), porque las hormonas exógenas cumplen funciones importantes en la morfogénesis de la planta en estas etapas (Sharmin et al 2014), lográndose la mayor regeneración de embriones a los 45 días de exposición a BAP 0.5 mg.L -1 (Fig. 4), mientras que mayores concentraciones de citoquinina y la combinación citoquinina-auxina redujeron la germinación.…”

Somatic Embryogenesis and Plant Regeneration in Sapindus mukorossi Gaertn. from Leaf-Derived Callus Induced with 6-Benzylaminopurine

“…c, d A well-acclimatized plant of Sapindus mukorossi developed from somatic embryo medium containing 8.88 μM BA than on medium without BA ( Table 5). Germination of somatic embryos in BA-containing medium was also reported in Wedelia calendulacea [38]. The synthesis and accumulation of storage and late embryogenesis abundant proteins during somatic embryo maturation was critical for the germination of somatic embryos [12].…”