Non-linear dynamics of chromosome condensation induced by colcemid

Fernandes, Thiago de Salazar e; Silva, Isvânia Maria Serafim da; Moraes, Renato Barros; Aguiar, Leandro A. A.; Amaral, Ademir; Barbosa, Catão T.F.; Nogueira, Rejane Jurema Mansur Custódio

doi:10.1590/s1516-89132013000100011

Cited by 5 publications

(2 citation statements)

References 26 publications

(41 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, cell synchrony protocols are needed. Importantly, commonly used nocodazole or colcemid treatments are not suitable for studying chromosome morphology, as both treatments delay cells in prometaphase and induce hyper-condensation of the chromosomes ( Hudson et al, 2003 ; Fernandes et al, 2013 ).…”

Section: Introductionmentioning

confidence: 99%

Functional analysis after rapid degradation of condensins and 3D-EM reveals chromatin volume is uncoupled from chromosome architecture in mitosis

Samejima

Booth

Ogawa

et al. 2018

Journal of Cell Science

View full text Add to dashboard Cite

The requirement for condensin in chromosome formation in somatic cells remains unclear, as imperfectly condensed chromosomes do form in cells depleted of condensin by conventional methodologies. In order to dissect the roles of condensin at different stages of vertebrate mitosis, we have established a versatile cellular system that combines auxin-mediated rapid degradation with chemical genetics to obtain near-synchronous mitotic entry of chicken DT40 cells in the presence and absence of condensin. We analyzed the outcome by live- and fixed-cell microscopy methods, including serial block face scanning electron microscopy with digital reconstruction. Following rapid depletion of condensin, chromosomal defects were much more obvious than those seen after a slow depletion of condensin. The total mitotic chromatin volume was similar to that in control cells, but a single mass of mitotic chromosomes was clustered at one side of a bent mitotic spindle. Cultures arrest at prometaphase, eventually exiting mitosis without segregating chromosomes. Experiments where the auxin concentration was titrated showed that different condensin levels are required for anaphase chromosome segregation and formation of a normal chromosome architecture.This article has an associated First Person interview with the first author of the paper.

show abstract

Section: Introductionmentioning

confidence: 99%

Functional analysis after rapid degradation of condensins and 3D-EM reveals chromatin volume is uncoupled from chromosome architecture in mitosis

Samejima

Booth

Ogawa

et al. 2018

Journal of Cell Science

View full text Add to dashboard Cite

show abstract

“…The DFA is one of the most widely used methods for the identification of LRTC in time series especially in being able to quantify scales in non-stationary series such as the EEG ( 22 ). Studies have successfully used it to identify changes in EEG patterns regarding neurodegenerative diseases such as Alzheimer disease ( 15 ).…”

Section: Introductionmentioning

confidence: 99%

Long-term correlation of the electrocorticogram as a bioindicator of brain exposure to ionizing radiation

Aguiar

Silva

Fernandes

et al. 2015

Braz J Med Biol Res

View full text Add to dashboard Cite

Understanding the effects of radiation and its possible influence on the nervous system are of great clinical interest. However, there have been few electrophysiological studies on brain activity after exposure to ionizing radiation (IR). A new methodological approach regarding the assessment of the possible effects of IR on brain activity is the use of linear and nonlinear mathematical methods in the analysis of complex time series, such as brain oscillations measured using the electrocorticogram (ECoG). The objective of this study was to use linear and nonlinear mathematical methods as biomarkers of gamma radiation regarding cortical electrical activity. Adult Wistar rats were divided into 3 groups: 1 control and 2 irradiated groups, evaluated at 24 h (IR24) and 90 days (IR90) after exposure to 18 Gy of gamma radiation from a cobalt-60 radiotherapy source. The ECoG was analyzed using power spectrum methods for the calculation of the power of delta, theta, alpha and beta rhythms and by means of the α-exponent of the detrended fluctuation analysis (DFA). Using both mathematical methods it was possible to identify changes in the ECoG, and to identify significant changes in the pattern of the recording at 24 h after irradiation. Some of these changes were persistent at 90 days after exposure to IR. In particular, the theta wave using the two methods showed higher sensitivity than other waves, suggesting that it is a possible biomarker of exposure to IR.

show abstract

An improved technique for obtaining well-spread metaphases from plants with numerous large chromosomes

Rodríguez-Domínguez

Ríos-Lara

Tapia‐Campos

et al. 2017

Biotechnic & Histochemistry

View full text Add to dashboard Cite

Preparations that contain well-spread metaphase chromosomes are critical for plant cytogenetic analyses including chromosome counts, banding procedures, in situ hybridization, karyotyping and construction of ideograms. Chromosome spreading is difficult for plants with large and numerous chromosomes. We report here a technique for obtaining cytoplasm-free, well-spread metaphases from two Amaryllidaceae species: Sprekelia formosissima (2n = 120) and Hymenocallis howardii (2n = 96). The technique has three main steps: 1) pretreatment to cause chromosome condensation, 2) dripping onto tilted slides coated with a thin layer of pure acetic acid and 3) application of steam and acetic acid to produce cytoplasmic hydrolysis, which spreads the chromosomes.

show abstract

Non-linear dynamics of chromosome condensation induced by colcemid

Abstract: ABSTRACT

Cited by 5 publications

References 26 publications

Functional analysis after rapid degradation of condensins and 3D-EM reveals chromatin volume is uncoupled from chromosome architecture in mitosis

Functional analysis after rapid degradation of condensins and 3D-EM reveals chromatin volume is uncoupled from chromosome architecture in mitosis

Long-term correlation of the electrocorticogram as a bioindicator of brain exposure to ionizing radiation

An improved technique for obtaining well-spread metaphases from plants with numerous large chromosomes

Contact Info

Product

Resources

About