Efficient Regeneration of Eucalyptus urophylla x Eucalyptus grandis from Stem Segment

Abstract

The aim of the present study was to establish an efficient regeneration system for the hybrid E. urophylla ×E. grandis by means of organogenesis. Stem segments from seedlings were used as explants and cultured in a modified Murashige and Skoog medium (MS), supplemented with 13.2 µM N-phenyl-N'-[6-(2-chlorobenzothiazol)-yl] urea (PBU) and 0.285 µM indole-3-acetic acid (IAA). PBU was a useful growth regulator. After cultivating for 5 d, 96% explants formed callus. After 30 d, the calli obtained were transferred to MS medium containing different combinations of 6-benzyladenine (BA) and naphthalene acetic acid (NAA

Physiological parameters and differential expression analysis of N-phenyl-N′-[6-(2-chlorobenzothiazol)-yl] urea-induced callus of Eucalyptus urophylla × Eucalyptus grandis

“…However, for most plants, Agrobacterium tumefaciens-mediated transformation depends on the effectiveness of the tissue culture methods used, of which callus induction is the initial step (Li & Luo, 2001). In this regard, few studies have reported the successful regeneration of E. grandis × E. urophylla via callus propagation (Ouyang et al, 2012;Ouyang & Li, 2016).…”

“…Synthetic phenylurea derivatives are potent plant growth regulators that exhibit cytokinin-like activity in various culture systems (Chung, Chen & Chang, 2007;Werner & Schmülling, 2009;Turker, Yucesan & Gurel, 2009;Huang et al, 2014;Liu et al, 2019), among which N-phenyl-N′-[6-(2-chlorobenzothiazol)-yl] urea (PBU) was first synthesized and purified in our laboratory (Li & Luo, 2001). It has been demonstrated that PBU is more efficient than 6-benzyladenine (6-BA) in Eucalyptus callus induction (Ouyang et al, 2012), and, moreover, PBU-induced callus shows a higher frequency of adventitious bud induction upon transfer to adventitious bud-inducing medium (Li, Ouyang & Gan, 2015).…”

Peer Review #2 of "Physiological parameters and differential expression analysis of N-phenyl-N′-[6-(2-chlorobenzothiazol)-yl] urea-induced callus of Eucalyptus urophylla × Eucalyptus grandis (v0.1)"

“…However, for most plants, Agrobacterium tumefaciens-mediated transformation depends on the effectiveness of the tissue culture methods used, of which callus induction is the initial step (Li and Luo, 2001). In this regard, few studies have reported the successful regeneration of E. grandis × E. urophylla via callus propagation (Ouyang et al, 2012;Ouyang and Li, 2016). Synthetic phenylurea derivatives are potent plant growth regulators that exhibit cytokinin-like activity in various culture systems (Chung et al, 2007;Werner and Schmülling, 2009;Turker et al, 2009;Huang et al, 2014;Liu et al, 2019), among which N-phenyl-Nʹ-[6-(2chlorobenzothiazol)-yl] urea (PBU) was first synthesized and purified in our laboratory (Li and Luo, 2001).…”

“…Synthetic phenylurea derivatives are potent plant growth regulators that exhibit cytokinin-like activity in various culture systems (Chung et al, 2007;Werner and Schmülling, 2009;Turker et al, 2009;Huang et al, 2014;Liu et al, 2019), among which N-phenyl-Nʹ-[6-(2chlorobenzothiazol)-yl] urea (PBU) was first synthesized and purified in our laboratory (Li and Luo, 2001). It has been demonstrated that PBU is more efficient than 6-benzyladenine (6-BA ) in Eucalyptus callus induction (Ouyang et al, 2012), and, moreover, PBU-induced callus shows a higher frequency of adventitious bud induction upon transfer to adventitious bud-inducing medium (Li et al, 2015). Although embryogenic callus differentiation is recognized as a key precursor to adventitious bud induction, the mechanisms underlying embryogenic callus differentiation in Eucalyptus have yet to be fully determined.…”

Somatic embryogenesis and plant regeneration in Wedelia calendulacea Less. an endangered medicinal plant

“…Thus, for sustainable utilization and to counter the over-exploitation and consequent threats to biodiversity, it should be envisioned to practice for mass multiplication and conservation of this valuable medicinal plant. In recent years, there has been an increased interest in in vitro culture techniques, which offer a viable tool for mass multiplication and germplasm conservation of rare, endangered and threatened medicinal plants (Radmann et al 2011;da Silva et al 2012;Ouyang et al 2012;Silveira et al 2013). Somatic embryogenesis has been proved to be useful and efficient method for mass clonal propagation of selected material, production of mutants and artificial seeds, genetic engineering and germplasm cryopreservation (Gonzalez-Arnao et al 2008;Hasbullah et al 2013).…”