Scale up of dextran production from a mutant of Pediococcus pentosaceus (SPAm) using optimized medium in a bioreactor

Patel, Seema; Kothari, Damini; Shukla, Rishikesh; Das, Debasish; Goyal, Arun

doi:10.1590/s1516-89132011000600007

Cited by 5 publications

(1 citation statement)

References 29 publications

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“…colonies has been used as a phenotypical test to select EPS-producing bacteria (Patel, Kothari, Shukla, Das, & Goyal, 2011). In addition, we have previously detected production of dextran by the meat LAB in solid medium containing sucrose (Nácher-Váquez et al, 2017).…”

Section: Macroscopic and Microscopic Detection Of Dextran Productionmentioning

confidence: 99%

Rheology and bioactivity of high molecular weight dextrans synthesised by lactic acid bacteria

Zarour¹,

Llamas

Prieto

et al. 2017

Carbohydrate Polymers

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Dextrans synthesised by three Leuconostoc mesenteroides strains, isolated from mammalian milks, were studied and compared with dextrans produced by Lc. mesenteroides and Lactobacillus sakei strains isolated from meat products. Size exclusion chromatography coupled with multiangle laser light scattering detection analysis demonstrated that the dextrans have molecular masses between 1.74×10Da and 4.41×10Da. Rheological analysis of aqueous solutions of the polymer revealed that all had a pseudoplastic behaviour under shear conditions and a random, and flexible, coil structure. The dextrans showed at shear zero a difference in viscosity, which increased as the concentration increased. Also, the purified dextrans were able to immunomodulate in vitro human macrophages, partially counteracting the inflammatory effect of Escherichia coli O111:B4 lipopolysaccharide. During prolonged incubation on a solid medium containing sucrose, dextran-producing bacteria showed two distinct phenotypes not related to the genus or species to which they belonged. Colonies of Lc. mesenteroides CM9 from milk and Lb. sakei MN1 from meat formed stable and compact mucoid colonies, whereas the colonies of the other three Leuconostoc strains became diffuse after 72h. This differential behaviour was also observed in the ability of the corresponding strains to bind to Caco-2 cells. Strains forming compact mucoid colonies showed a high level of adhesion when grown in the presence of glucose, which decreased in the presence of sucrose (the condition required for dextran synthesis). However no influence of the carbon source was detected for the adhesion ability of the other Lc. mesenteroides strains, which showed variable levels of binding to the enterocytes.

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Section: Macroscopic and Microscopic Detection Of Dextran Productionmentioning

confidence: 99%