Culture media selection for mass production of Isaria fumosorosea and Isaria farinosa

Mascarin, Gabriel Moura; Alves, Sérgio Batista; Lopes, Rogério Biaggioni

doi:10.1590/s1516-89132010000400002

Cited by 53 publications

(32 citation statements)

References 9 publications

(11 reference statements)

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“…Regarding the biphasic culture between the different substrates, the production of conidia per gram was maintained in a range of 10 5 to 10 8 and the production obtained showed significant differences between the isolates and also between the substrates used, registering the minimum of 7.60×10 5 conidia g -1 for the grain of birdseed and the maximum of 2.07 × 10 7 conidia g -1 for the grain of rice. These results coincide with those reported by Mascarin et al (2010) for biphasic culture with strains of I. farinosa and I. fumosorosea who report values in a range of 10 7 in different varieties of rice (whole, broken, white) as a substrate and different combinations of liquid media for the pre-inoculum phase. In another study Méndez et al (2009) carried out the production of Nomuraea rileyi by biphasic culture and reported values of 1.68 ×10 10 conidia g -1 , whereas in a study with Lecanicillium lecanii with this same type of culture, values of up to 1.49 × 10 9 conidia g -1 were obtained using combinations of liquid media obtained from agroindustrial waste and by-products and a solid phase prepared with mixtures of cereals and derivatives (RIBEIRO-MACHADO et al, 2010).…”

Section: Final Comparison Of the Conidia Production Of Isaria Fumosorsupporting

confidence: 91%

“…The heterogeneity of the results obtained in the present study with those reported in the literature may be due to several factors among which the technique used for propagation, the nutritional load of the substrate, as well as the capacity of the same isolate to degrade this substrate can be mentioned and multiply. Mascarin et al (2010) and Gao ;Liu et al (2010) mention that an important key aspect to choose a substrate for the production of fungi is to determine its chemical composition with emphasis on its carbon content and the carbon-nitrogen ratio (C: N), because being products or waste with plant origin their nutrients are considerably variable and will depend on the species and varieties of plants, harvest times as well as the farms and / or plots from which this material comes from the nutrients that may be available and this variation can greatly affect the mushroom production parameters, since there are no standard substrates in the market, however, a nutrient analysis for the different substrates, in order to control these variations, is recommended to ensure the quality of the substrates.…”

Section: Final Comparison Of the Conidia Production Of Isaria Fumosormentioning

confidence: 99%

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Conidia production of Isaria fumosorosea (Hypocreales: Cordycipitaceae) in organic substrates through two propagation techniques

et al. 2019

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In the last decades, the use of entomopathogenic fungi for the control of pest insects has increased globally, however a key step to achieve the successful application of fungal propagules as biocontrol agents depends of various factors, inoculum production being one of the essential stages for these microorganisms to be used in pest management programs. The objective of this study was to evaluate the production of conidia by solid fermentation and biphasic culture in different sporulation matrices for four native isolates and a strain of Isaria fumosorosea that have been shown potential for the control of various insects at the laboratory level. The experimental design was completely randomized, two propagation methods were used with six treatments and ten repetitions per treatment; the data were analyzed using an analysis of variance and the means were compared using the Tukey test (p≤ 0.05). In the production by solid fermentation the minimum value of 5.30 × 10 5 conidia g -1 was registered with the pericarp of peanut and the maximum of 2.35 × 10 7 conidia g -1 in the corn grain; in the biphasic culture the minimum of 7.60 × 10 5 conidia g -1 was observed in birdseed and the maximum of 2.07 × 10 7 conidia g -1 in rice. The differences were significant (p ≤ 0.05) in the production by method and by substrate, in solid fermentation 6.84 × 10 6 conidia g -1 and in biphasic culture 8.85 × 10 6 conidia g -1 . In the production by substrate, the rice showed 1.75 × 10 7 conidia g -1 and the lesser canary seed concentration (7.80 × 10 5 conidia g -1 ). The average production per isolate and / or strain was of the order of 10 6 and significant difference was registered (p ≤ 0.05) among the fungi, the isolate HIB-9 showed concentration in average higher in the production (7.90 × 10 6 conidia g -1 ) and the isolated HIB-19 was the least effective, with only 1.08 × 10 6 conidia g -1 . The results obtained show marked differences between the isolates with respect to their capacity to use the different substrates used as propagation media to obtain conidia of I. fumosorosea.

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Section: Final Comparison Of the Conidia Production Of Isaria Fumosorsupporting

confidence: 91%

Section: Final Comparison Of the Conidia Production Of Isaria Fumosormentioning

confidence: 99%

Conidia production of Isaria fumosorosea (Hypocreales: Cordycipitaceae) in organic substrates through two propagation techniques

et al. 2019

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“…Por tal motivo, para la producción de conidios se ha evaluado el efecto del tipo de cultivo, la naturaleza de los sustratos y el uso de inductores. Durante la producción de conidios en fermentación líquida (FL) se ha demostrado que las enzimas hidrolíticas pueden ser inducibles (Jiménez-Alejandro, 2016;Motesinos-Matías,2012;Safavi et al, 2007;Campos et al, 2005;Matsumoto et al, 2004) pero en dicho proceso los productos no tienen una alta efectividad para su uso a nivel agrícola; en cambio, los productos derivados de la fermentación sólida (FS) presentan una ventaja con respecto a su viabilidad, debido a la formación de conidios aéreos (López-Sosa, 2017;Mascarin et al, 2010;Lomer et al, 2001). En cuanto a los sustratos que se han utilizado predomina el uso de cereales como arroz y trigo por su amplia disponibilidad y composición química (Villaba, 2009;Méndez, 2010;Gandarilla-Pacheco, 2013).…”

Section: Introductionunclassified

Análisis cualitativo de la producción de enzimas de Beauveria bassiana en fermentación sólida utilizando un inductor

2018

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La producción de hongos entomopatógenos es un área de estudio de gran importancia representando una alternativa ante el uso de insecticidas de origen químico. Durante el mecanismo de infección los hongos entomopatógenos liberan lipasas, proteasas y quitinasas responsables de la penetración de la cutícula de los insectos. Se ha demostrado que en fermentación líquida estas enzimas son inducibles, pero los productos obtenidos por este bioproceso no tienen alta efectividad para su uso a nivel agrícola; en cambio, los obtenidos por fermentación sólida presentan una ventaja con respecto a su viabilidad, debido a la formación de conidios aéreos. El objetivo de este trabajo fue analizar cualitativamente la producción de enzimas de Beauveria bassiana en fermentación sólida utilizando mezclas de arroz y cutículas de T. molitor (100:0, 95:5, 90:10, 85:15 %). En las pruebas semicualitativas realizadas se observó que el uso de inductores en fermentación sólida no tuvo efecto con respecto a la represión total de las enzimas producidas. En cuanto a la virulencia de los conidios, el mejor tratamiento se obtuvo con una relación sustrato: inductor 90:10para producir conidios con mayor virulencia en los bioensayos.

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“…El ingrediente activo de este hongo son las esporas aéreas producidas a través de un sistema de fermentación bifásica (líquido-sólido) obteniendo concentraciones de esporas con toxicidad satisfactoria en un menor tiempo (Mascarin et al, 2010).…”

Section: Introductionunclassified

Estudio de las condiciones de mezclado en fermentador para la producción de blastosporas de Beauveria bassiana

García-Gutiérrez¹,

González-Maldonado²,

Medrano-Roldán³

et al. 2013

Rev. Colomb. Biotecnol.

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Estudio de las condiciones de mezclado en fermentador para la producción de ResumenSe caracterizaron tres fermentadores: New Brunswick M-19 de 14 litros, Applikon Biocontroller 1035 de 7 litros y New Brunswick Bioflo III de 7 litros, determinando el coeficiente volumétrico de transferencia de oxígeno (KLa), la retención de gas (RG) y el tiempo de mezclado (tM). El fermentador New Brunswick Bioflo III tuvo los mejores valores con una relación de diámetro del impulsor/diámetro del tanque (DI/DT) de 0.43, KLa = 9.5-208 h -1 y tM = 1.0-3.0 s, por lo que fue seleccionado para realizar la producción de blastosporas de Beauveria bassiana, utilizando melaza como fuente de carbono. Se estudiaron las condiciones de mezclado, utilizando diferentes combinaciones de impulsores tipo Rushton, Maxflo y Lightnin, bajo un diseño experimental factorial 3 2 . El tiempo de propagación fue de 4 días, el volumen de trabajo 4 litros, 10% de inóculo (1x10 6 blastosporas/ml), temperatura 30°C, agitación de 400-500 rpm, aireación de 0.5-1.0 vvm, y pH de 5.4.El hongo se desarrolló mejor utilizando la combinación de impulsores Rushton-Maxflo a 400 rpm y 1.0 vvm (F = 10.324, p ≤ 0.0123) (DMS=0.585), obteniendo una concentración de 1.2x10 9 blastosporas/ml, 2.2 g/l de biomasa y 2.48 g/l de consumo de sustrato (Y x/s=0.89). Las condiciones de mezclado y los parámetros obtenidos pueden ser aplicados en otros fermentadores para optimizar la producción de blastosporas de B. bassiana en la elaboración experimental de bioinsecticidas.Palabras clave: fermentación líquida, aireación, agitación, hongo entomopatógeno. AbstractIn this work three fermenters were characterized: New Brunswick M-19 of 14 liters, Biocontroller Applikon 1035 of 7 liters and New Brunswick Bioflo III of 7 liters, determining the volumetric coefficient oxygen transfer (KLa), gas hold up (GH) and the mixing time (tM). The fermenter New Brunswick Bioflo III had the best values with a ratio of diameter of the impeller/ vessel (DI/DT) of 0.43, KLa = 9.5-208 h -1 and tM = 1.0-3.0 s, so it was selected for the production of blastospores of Beauveria bassiana, using molasses as carbon source. We studied the mixing conditions, using different combinations of impellers, type Rushton, Lightnin and Maxflo under a factorial experimental design 3 2 . The propagation time was of 4 days, working volume 4l, 10% inoculum (1x10 6 blastospores/ml), temperature 30°C, agitation of 400-500 rpm, aeration 0.5-1.0 vvm, and pH of 5.4. The fungus growth better using a combination of impellers Rushton-Maxflo at 400 rpm and 1.0 vvm (F = 10.324, p ≤ 0.0123) (LSD = 0.585) obtaining a concentration of 1.2x10 9 blastospores/ml, 2.2 g/l biomass and 2.48 g/l of substrate consumption (Y x/s = 0.89). The mixing conditions and the parameters obtained can be applied to optimize the blastospore productions of B. bassiana to fermenter level in the experimental production of bioinsecticides.

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Culture media selection for mass production of Isaria fumosorosea and Isaria farinosa

Abstract: This work investigated the production of the fungi

Cited by 53 publications

References 9 publications

Conidia production of Isaria fumosorosea (Hypocreales: Cordycipitaceae) in organic substrates through two propagation techniques

Conidia production of Isaria fumosorosea (Hypocreales: Cordycipitaceae) in organic substrates through two propagation techniques

Análisis cualitativo de la producción de enzimas de Beauveria bassiana en fermentación sólida utilizando un inductor

Estudio de las condiciones de mezclado en fermentador para la producción de blastosporas de Beauveria bassiana

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