Functional cytology of the hepatopancreas of Palaemonetes argentinus (Crustacea, Decapoda, Caridea) under osmotic stress

Díaz, Ana Cristina; Sousa, Liliana G; Petriella, Ana María

doi:10.1590/s1516-89132010000300013

Cited by 32 publications

(19 citation statements)

References 20 publications

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“…In the present study, P‐gp gene expression was analysed in different tissues of the control, non‐medicated shrimp showed strong expression in the hepatopancreas, moderate expression in the intestine and muscle and low expression in other tissues, similar to the observations in white shrimp (Zhou et al, ). We can infer that the high P‐gp expression level in the hepatopancreas might reflect the fact this organ is responsible for absorption and detoxification (Johnston & Yellowlees, ; Miyawaki et al, ) and plays a role in the synthesis of digestive enzymes and the detoxification of drugs in crustaceans (Díaz, Sousa, & Petriella, ; Vogt, ). Furthermore, we determined the variability of P‐gp gene expression level in hepatopancreas, muscle and gill tissues after treatment with different concentration of OXA.…”

Section: Discussionmentioning

confidence: 99%

Pharmacokinetic/pharmacodynamic properties and P‐glycoprotein expression in pacific white shrimp, Litopenaeus vannamei after oral administration at three dosages of oxolinic acid

Yin

Wang

et al. 2019

Aquac Res

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The experiments explored the pharmacokinetics (PK) properties of oxolinic acid (OXA) after oral administration at three dosages (10, 30 and 80 mg/kg) via medicated feed in the shrimp. The results showed that the Cmax values of 4.31, 14.93 and 16.62 mg/L and AUC0–∞ values of 92.61, 252.30 and 364.27 mg hr−1 L−1 were observed at three OXA dosage groups in the haemolymph respectively. In the hepatopancreas, Cmax values of 7.90, 27.23 and 60.51 mg/kg and AUC0–∞ values of 42.01, 133.06 and 219.06 mg hr−1 L−1 were observed at 0.5 hr post administration respectively. In the muscle, Cmax values of 1.62, 5.80 and 7.36 mg/kg and the AUC0–∞ values of 25.64, 98.10 and 134.24 mg hr−1 L−1 were observed at 2 hr post administration respectively. In the gills, Cmax values of 2.87, 8.08 and 12.12 mg/kg and the AUC0–∞ values of 51.38, 118.65 and 206.48 mg hr−1 L−1 were observed at 4 hr post administration respectively. In addition, the in vitro MIC values of OXA at three dosages against 132 strains of Vibrio were examined and showed that the minimum inhibitory concentration (MIC) values for OXA primarily ranged from 0.15–1.25 µg/ml, including eight strains of Vibrio showing MIC values ≥5 µg/ml. The MIC50 and MIC90 values of 132 strains were 0.62 and 1.25 μg/ml respectively. The AUC0–24/MIC90 ratios of Vibrio were 140.4 in 30 mg/kg group. Furthermore, the P‐glycoprotein (P‐gp) expression was determined in shrimp tissues after administration to three dosage groups (10, 30 and 80 mg/kg). The results showed that P‐gp expression was up‐regulated in the hepatopancreas (5.36‐, 13.68‐ and 31.06‐fold respectively) compared with the control group.

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Section: Discussionmentioning

confidence: 99%

Pharmacokinetic/pharmacodynamic properties and P‐glycoprotein expression in pacific white shrimp, Litopenaeus vannamei after oral administration at three dosages of oxolinic acid

Yin

Wang

et al. 2019

Aquac Res

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“…Morphological alterations of the HP, such as reduction in the tubular area and enlargement of the lumen area, variation in the number of B‐cells, F‐cells and R‐cells, infiltration of haemocytes in the intertubular spaces, have been described by different authors in case of pathogens, food deprivation, changes in salinity and food ingredients (Nakamura ; Sánchez‐Paz et al . ; Tam & Avenant‐Oldewage ; Diaz, Sousa & Petriella ; Masson, Diaz & Petriella ; Sacristán et al . ).…”

Section: Methodsmentioning

confidence: 99%

Use of computer‐assisted image analysis for semi‐quantitative histology of the hepatopancreas in whiteleg shrimp Penaeus vannamei (Boone)

Cervellione

McGurk

Eriksen

et al. 2016

Journal of Fish Diseases

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Despite the increasing use of novel molecular techniques in pathology, histology remains the standard method for monitoring tissue alterations and for assessing pathology. Histopathological evaluation is generally laborious and subjective with risk of discrepancies in semi-quantitative scoring between pathologists. In contrast, computer-assisted image analysis (CAIA) is potentially faster, more objective and thus suitable for routine screening. Limited research has been carried out on CAIA in crustacean histopathology, and the methods described were not fully automated. Therefore, the aim of this study was to develop CAIA in whiteleg shrimp (Penaeus vannamei) for the study of the hepatopancreas. Paraffin sections were immunohistochemically stained with monoclonal antibodies WSH8 against haemocytes and counterstained with Mayer's haematoxylin for detection of haemocytes and B-cell vacuoles, and modified toluidine blue protocol was used for detection of F-cells; frozen sections were stained with Oil Red O for detection of lipid droplets within R-cells. Visiopharm software was used to develop and validated protocols for the quantification of morphological parameters (areas of haemocyte infiltration, F-cells, B-cell vacuoles, lipid droplets and their ratios to total tissue area and total lumen area). These protocols enable the future use of CAIA for determination of the nutritional and pathological condition of this organ.

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“…Estos resultados sugieren que en los camarones adultos expuestos a salinidad de 4‰ se estimularía el metabolismo o la asimilación de nutrientes, como lo sucedido en Palaemonetes argentinus, expuesto en 8‰ de salinidad (Díaz et al, 2010).…”

Section: Escobar M Pachamoro Y W Reyesunclassified

“…Sin embargo, estos resultados son contrarios a lo registrado en M. rosenbergii, donde los adultos pierden habilidad para hiperregular a 17‰ de salinidad (Cheng et al, 2003). En P. argentinus, el incremento de la salinidad del agua (8‰ a 24‰) ocasiona reducción de la función metabólica y alteraciones citológicas en el hepatopáncreas (Díaz et al, 2010). En adultos de M. rosenbergii la mortalidad es asociada con la inhabilidad para mudar en agua con 25‰ de salinidad (Huong et al, 2010;Chand et al, 2015).…”

Section: Escobar M Pachamoro Y W Reyesunclassified

SUPERVIVENCIA Y CRECIMIENTO DE MACHOS ADULTOS DEL CAMARÓN DE RÍO Cryphiops caementarius Molina, 1782 (CRUSTACEA, PALAEMONIDAE) EXPUESTOS A SALINIDADES

2017

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ResumenEl objetivo fue evaluar la supervivencia y el crecimiento de machos adultos del camarón de río Cryphiops caementarius expuestos a salinidades. El experimento se realizó en 12 acuarios con 50 l de agua, cada uno con filtro biológico y con cinco refugios. Las salinidades experimentales fueron 0, 2, 4 y 8‰, implementadas por triplicado. En cada acuario se sembró al azar cinco camarones. Un total de 60 camarones machos adultos (5.94 ± 0.11 cm de longitud total y 8.15 ± 0.810 g de peso húmedo) fueron usados en el experimento que duró 60 días. Los camarones expuestos en agua con 4‰ de salinidad presentaron alta y significativa (p<0.05) supervivencia (>80%), en cambio en 0‰ y 2‰ la supervivencia fue baja (20%) debido al canibalismo y en 8‰ la supervivencia fue muy baja (6%) debido al estrés osmótico. El crecimiento de los camarones expuestos en salinidad de 4‰ no mostró diferencia significativa (p>0.05) con aquellos expuestos en 0‰ y en 2‰ de salinidad durante el período de estudio, pero los mayores valores de crecimiento en peso se presentaron en 4‰ de salinidad. Palabras clave: estrés osmótico, crecimiento, osmorregulación, supervivencia, Cryphiops. AbstractThe aim was to evaluate the survival and growth of adult males of the freshwater shrimp Cryphiops caementarius exposed to salinities. The experiment was carried out in 12 aquariums with 50 l of water, each with biological filter and five shelters. The experimental salinities were 0, 2, 4 and 8‰, implemented in triplicate. Five shrimps were stocked at random in each aquarium. A total of 60 adult male shrimps (5.94 ± 0.11 cm of total length and 8.15 ± 0.810 g of wet weight) were used in the experiment that lasted 60 days. Shrimps exposed to water with 4‰ of salinity presented high and significant (p<0.05) survival (>80%), whereas in 0 ‰ and 2 ‰ the survival was low (20%) due to cannibalism, and in 8‰ the survival was very low (6%) due to osmotic stress. Growth of shrimps exposed to 4‰ salinity showed no significant difference (p>0.05) with those exposed to 0‰ and 2‰ of salinity during the study period, but the highest values of growth in weight were presented to 4‰ salinity.

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Functional cytology of the hepatopancreas of Palaemonetes argentinus (Crustacea, Decapoda, Caridea) under osmotic stress

Abstract: ABSTRACT

Cited by 32 publications

References 20 publications

Pharmacokinetic/pharmacodynamic properties and P‐glycoprotein expression in pacific white shrimp, Litopenaeus vannamei after oral administration at three dosages of oxolinic acid

Pharmacokinetic/pharmacodynamic properties and P‐glycoprotein expression in pacific white shrimp, Litopenaeus vannamei after oral administration at three dosages of oxolinic acid

Use of computer‐assisted image analysis for semi‐quantitative histology of the hepatopancreas in whiteleg shrimp Penaeus vannamei (Boone)

SUPERVIVENCIA Y CRECIMIENTO DE MACHOS ADULTOS DEL CAMARÓN DE RÍO Cryphiops caementarius Molina, 1782 (CRUSTACEA, PALAEMONIDAE) EXPUESTOS A SALINIDADES

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