Nodulation and N2 fixation effectiveness of Bradyrhizobium strains in symbiosis with Adzuki Bean, Vigna angularis

Delić, Dušica; Stajković, Olivera; Rasulić, Nataša; Kuzmanović, Đorđe; Jošić, Dragana; Miličić, Bogić

doi:10.1590/s1516-89132010000200007

Cited by 22 publications

(12 citation statements)

References 15 publications

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“…The significant correlation between nodule dry weight and shoot dry weight in the greenhouse experiments confirmed the dependence of shoot biomass on nodulation (Kawaka et al, 2014 ). In addition, the results from this study support the assertion made by Delić et al ( 2010 ), that there is a direct relationship between nodulation and nitrogen accumulation in legumes. Our findings, therefore, demonstrated that rhizobia inoculation enhanced nodulation and nitrogen fixation, which improved shoot nitrogen nutrition and plant biomass.…”

Section: Discussionsupporting

confidence: 91%

Potential of Native Rhizobia in Enhancing Nitrogen Fixation and Yields of Climbing Beans (Phaseolus vulgaris L.) in Contrasting Environments of Eastern Kenya

et al. 2017

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Climbing bean (Phaseolus vulgaris L.) production in Kenya is greatly undermined by low soil fertility, especially in agriculturally prolific areas. The use of effective native rhizobia inoculants to promote nitrogen fixation could be beneficial in climbing bean production. In this study, we carried out greenhouse and field experiments to evaluate symbiotic efficiency, compare the effect of native rhizobia and commercial inoculant on nodulation, growth and yield parameters of mid-altitude climbing bean (MAC 13 and MAC 64) varieties. The greenhouse experiment included nine native rhizobia isolates, a consortium of native isolates, commercial inoculant Biofix, a mixture of native isolates + Biofix, nitrogen treated control and a non-inoculated control. In the field experiments, the treatments included the best effective native rhizobia isolate ELM3, a consortium of native isolates, a commercial inoculant Biofix, a mixture of native isolates + Biofix, and a non-inoculated control. Remarkably, four native rhizobia isolates ELM3, ELM4, ELM5, and ELM8 showed higher symbiotic efficiencies compared to the Biofix. Interestingly, there was no significant difference in symbiotic efficiency between the two climbing bean varieties. Field results demonstrated a significant improvement in nodule dry weight and seed yields of MAC 13 and MAC 64 climbing bean varieties upon rhizobia inoculation when compared to the non-inoculated controls. Inoculation with ELM3 isolate resulted to the highest seed yield of 4,397.75 kg ha−1, indicating 89% increase over non-inoculated control (2,334.81 kg ha−1) and 30% increase over Biofix (3,698.79 kg ha−1). Farm site significantly influenced nodule dry weight and seed yields. This study, therefore, revealed the potential of native rhizobia isolates to enhance delivery of agroecosystem services including nitrogen fixation and bean production. Further characterization and mapping of the native isolates will be imperative in development of effective and affordable commercial inoculants.

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Section: Discussionsupporting

confidence: 91%

Potential of Native Rhizobia in Enhancing Nitrogen Fixation and Yields of Climbing Beans (Phaseolus vulgaris L.) in Contrasting Environments of Eastern Kenya

et al. 2017

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“…The dark green color observed in some of the inoculated treatments and not in the un-inoculated control showed effective symbiotic relationship between the common bean plant and some of the isolates after the sixth week of development. This corresponds well with previous works [56]. Strain 446 was poor in infectiveness and effectiveness as shown in Fig.…”

Section: Authentication Of Rhizobia Isolatessupporting

confidence: 92%

“…This is in agreement with previous work, that has shown that nodule number and nodule dry weight is not appropriate for determining the effectiveness of a rhizobia -legume association [47,56]. The significant differences in the shoot dry weights show clear differences in the ability of the isolates to fix nitrogen and are among the preferred methods for determining symbiotic effectiveness of rhizobia isolates [57].…”

Section: Authentication Of Rhizobia Isolatessupporting

confidence: 91%

Morphological Assessment and Effectiveness of Indigenous Rhizobia Isolates that Nodulate P. vulgaris in Water Hyacinth Compost Testing Field in Lake Victoria Basin

Muthini

2014

BJAST

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Aims: The study was aimed at isolating, identifying and assessing the effectiveness of indigenous rhizobia nodulating P. vulgaris in Lake Victoria Basin (LVB). Study design: Randomized complete block design. Place and Duration of Study: Soil and nodule samples were collected from Kisumu (Kenya); Kabanyolo (Uganda) and Nyabarongo (Rwanda). Field experiments: Kisumu (Kenya). Lab and greenhouse experiments: Department of Plant and Microbial Sciences Kenyatta University (Kenya) and Makerere University (Uganda). Research was carried out between January 2012 and April 2013. Methodology: Rhizobia were isolated from nodules obtained from P. vulgaris (rose coco variety) plants planted in the LVB water hyacinth compost trial fields and whole soil trapping experiments in the greenhouse using soil obtained from the LVB. The isolates were identified using morphological characteristics. Isolates from each group were used in authentication using the infection technique. Results: One hundred and twenty eight isolates were obtained from the trapping experiments and placed into nine groups based on their morphological characteristics. Four hundred and seventy two isolates were obtained from the nodules of the P. vulgaris grown in soils amended with water hyacinth compost and were placed into sixteen groups. The isolates varied in their morphological characteristics. There was a significant difference in the infectiveness and effectiveness of the representative rhizobia isolates. Conclusion: The studies revealed that rhizobia isolates from Lake Victoria are different morphologically. Authentication experiments, confirmed that the majority of the isolates were rhizobia due to their ability to infect the host plant P. vulgaris. All representative isolates varied in their ability to infect and fix nitrogen. Isolates that are more effective compared to the commercial Rhizobium leguminosarum biovar phaseoli strain 446 were identified in this study. The effective indigenous rhizobia have therefore the potential of being sources of inocula for P. vulgaris.

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“…(Ribeiro et al, 2015) and Vigna angularis (Willd.) Ohwiand H. Ohashi (Delić et al, 2010), but reduced in relation to strains for Phaseolus lunatus L. (Antunes et al, 2011) and Cajanus cajan(L.) Huth .…”

Section: Discussionmentioning

confidence: 95%

Response of Cratylia argentea (Desvaux) O. Kuntzeto inoculation with Rhizobium sp. and Bradyrhizobium sp. strains

Mattar¹,

Barros²,

Brasileiro³

et al. 2018

Aust J Crop Sci

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Cratylia argentea is a neotropical leguminous shrub that exhibits symbiosis with nitrogen (N) fixing bacteria and high levels of protein. Thus, it has potential for animal feed and green manure. In spite of its potential, few cultivars are registered for the species and there is little information in regard to its interaction with N-fixing microorganisms. The aim of this study was to evaluate the effect of inoculation with rhizobium strains on the growth and concentration of N in Cratylia argentea. Seeds were inoculated with two strains of Rhizobium sp.,CR 33 (BR10243) and CR42 (BR10244), and four strains of Bradyrhizobium sp., CR52 (BR10257), CPAC-B10, SEMIA 6464 (BR3262), and SEMIA 6462 (BR3267), and sown in pots filled with sand and vermiculite in a greenhouse. After 150 days we evaluated the number of nodules, shoot and nodules dry matter, total nitrogen and crude protein contents for the shoots. The plants inoculated with CR 52 exhibited nodulation, with nodular efficiency of 0.189 and relative efficiency of 66.14%; it is possible to observe the effect of nodulation as of 85 days after sowing. Based on the obtained results it was identified the strain that showed symbiosis with C. argentea and presents potential to be used as a commercial inoculant.

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Nodulation and N2 fixation effectiveness of Bradyrhizobium strains in symbiosis with Adzuki Bean, Vigna angularis

Abstract: ABSTRACT

Cited by 22 publications

References 15 publications

Potential of Native Rhizobia in Enhancing Nitrogen Fixation and Yields of Climbing Beans (Phaseolus vulgaris L.) in Contrasting Environments of Eastern Kenya

Potential of Native Rhizobia in Enhancing Nitrogen Fixation and Yields of Climbing Beans (Phaseolus vulgaris L.) in Contrasting Environments of Eastern Kenya

Morphological Assessment and Effectiveness of Indigenous Rhizobia Isolates that Nodulate P. vulgaris in Water Hyacinth Compost Testing Field in Lake Victoria Basin

Response of Cratylia argentea (Desvaux) O. Kuntzeto inoculation with Rhizobium sp. and Bradyrhizobium sp. strains

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