A simplified model for A. Niger FS3 growth during phytase formation in solid State fermentation

Spier, Michele Rigon; Letti, Luiz Alberto Júnior; Woiciechowski, Adenise Lorenci; Soccol, Carlos Ricardo

doi:10.1590/s1516-89132009000700020

Cited by 7 publications

(2 citation statements)

References 30 publications

(18 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…have also been reported by other authors using different fungal strains. Spier et al (2009) observed good correlation (R 2 = 0.9863) between these variables during the phytase production by solid state fermentation with A. niger FS3. Ng et al (2008) found also good correlation between dry biomass and ergosterol content (R 2 = 0.9645) during the cultivation of A. niger ATCC 24126 in grains and feeds.…”

Section: Correlation Between Dry Weight and Ergosterol Contentmentioning

confidence: 74%

“…Other studies with different fungal strains reported also correlation between the phytase production and Research, Society and Development, v. 11, n. 6, e41311628994. 2022 (CC BY 4.0) | ISSN 2525-3409 | DOI: http://dx.doi.org/10.33448/rsd-v11i6.28994 microorganism growth (Krishna & Nokes, 2001;Spier et al, 2009;Spier et al, 2010). According to these studies, the ergosterol content can be considered a good indicator for biomass.…”

Section: Biomass Contentmentioning

confidence: 99%

See 1 more Smart Citation

Effect of pH and temperature on phytase and biomass production by submerged fermentation with Aspergillus niger var. phoenicis URM 4924

Nascimento

Ribeiro

Pessoa

et al. 2022

RSD

View full text Add to dashboard Cite

The production of phytase and biomass (estimated by the ergosterol content) by submerged fermentation with Aspergillus niger var. phoenicis URM 4924 was studied. Experimental assays were carried under different conditions of pH (4.0 to 8.0) and temperature (25 to 35 ºC), and the influence of these variables on the responses was studied through a 22 central composite design and response surface methodology. Phytase and biomass production were strongly affected by the pH and temperature used during fermentation. Phytase activity was increased in up to 7.8-fold (from 1.04 to 8.09 U/mL) and the ergosterol content was increased in up to 38-fold (from 9.3 to 354.09 µg/mL). The maximum values of both responses were achieved when using pH 4.0 and 30 ºC. Good correlation (second-order fit, R2 = 0.9875) was found between the data obtained for phytase activity and ergosterol content, suggesting that the phytase production depends on the biomass formation. These results are of interest since they contribute for the development of an industrial process for phytase production with elevated yields by submerged fermentation.

show abstract

Section: Correlation Between Dry Weight and Ergosterol Contentmentioning

confidence: 74%

Section: Biomass Contentmentioning

confidence: 99%

Effect of pH and temperature on phytase and biomass production by submerged fermentation with Aspergillus niger var. phoenicis URM 4924

Nascimento

Ribeiro

Pessoa

et al. 2022

RSD

View full text Add to dashboard Cite

show abstract

A bioprocess for the production of phytase from Schizophyllum commune: studies of its optimization, profile of fermentation parameters, characterization and stability

Salmon

Piva

Binati

et al. 2012

Bioprocess Biosyst Eng

View full text Add to dashboard Cite

Schizophyllum commune produces phytase through solid-state fermentation using different agroindustrial residues. After optimization of phytase production, a maximal level of phytase (113.7 Units/gram of dry substrate) was obtained in wheat bran based medium containing 5% sucrose, 50% humidity, 7.5% of biomass at 33 °C pH 7.0 during 72 h and a 285% improvement in enzyme titre was achieved. Analysis of fermentation parameters profile for phytase production showed the highest productivity (1.466 Units/gram of dry substrate/hour) in 66 h of fermentation. Phytase has an optimal pH of 5.0, an optimal temperature of 50 °C and K (m) and V (max) values of 0.16 mM and 1.85 μmol mL(-1) min(-1), respectively. Phytase activity was stimulated essentially in the presence of K(+), Ca(2+), Mg(2+), Mn(2+), Zn(2+), Cu(2+), Fe(2+), Fe(3+), Co(2+), Ni(2+), acetate and citrate at concentrations of 1 mM. Phytase had the best shelf life when stored at a cooling temperature, maintaining 38% of its initial activity after 112 days of storage, and still presenting enzymatic activity after 125 days of storage. Stability studies of phytase performed in aqueous enzyme extracts showed satisfactory results using polyethyleneglycol 3350, carboxymethylcellulose, methylparaben, mannitol and benzoic acid in concentrations of 0.25, 0.025, 0.025, 0.25, and 0.0025%, respectively. PEG 3350 was shown to be the best stabilizing agent, resulting in 109% of phytase activity from the initial crude extract remaining activity in after 90 days.

show abstract

Kinetic analysis of the uptake of glucose and corn oil used as carbon sources in batch cultures of Gibberella fujikuroi

Rios‐Iribe¹,

Hernández‐Calderón²,

Escamilla‐Silva

2016

World J Microbiol Biotechnol

View full text Add to dashboard Cite

This study determined the specific uptake rate of glucose and corn oil substrates used as carbon sources in batch cultures of Gibberella fujikuroi. We tested three biological models of growth rate: Monod, logistic and lag-exponential. With respect to the substrate consumption rate, we tested two models: constant cell yield (CCY) and law of mass action (LMA). The experimental data obtained from the culture with glucose as substrate correlated satisfactorily with the logistic/LMA model, indicating that the cell yield was variable. In the case of corn oil as carbon source, considering total residual lipids as substrate in the culture broth, the model with the best correlation was the lag-exp/CCY model. The quantification by GC of the three main fatty acids (linoleic, oleic and palmitic) in the culture medium showed a cumulative behavior, with a maximum concentration of each acid at 36 h. We established a more explicit mechanism of the consumption of corn oil, consisting of two stages: generation of fatty acids by hydrolysis and consumption by cellular uptake. The kinetic of hydrolysable lipids was of first order. We found that the hydrolysis rate of corn oil is not a limiting factor for the uptake of fatty acids by the microorganism. We also established, based on the analysis of the identical mathematical structure of consumption kinetics, that the uptake of fatty acids is faster than the uptake of glucose.

show abstract

A simplified model for A. Niger FS3 growth during phytase formation in solid State fermentation

Cited by 7 publications

References 30 publications

Effect of pH and temperature on phytase and biomass production by submerged fermentation with Aspergillus niger var. phoenicis URM 4924

Effect of pH and temperature on phytase and biomass production by submerged fermentation with Aspergillus niger var. phoenicis URM 4924

A bioprocess for the production of phytase from Schizophyllum commune: studies of its optimization, profile of fermentation parameters, characterization and stability

Kinetic analysis of the uptake of glucose and corn oil used as carbon sources in batch cultures of Gibberella fujikuroi

Contact Info

Product

Resources

About