Selective agent and A. tumefaciens overgrowth-control antibiotics in Eucalyptus camaldulensis Cotiledonary culture

Quisen, R. C.; Oliveira, Yohana de; Pileggi, Marcos; Cuquel, Francine Lorena; Quoirin, Marguerite

doi:10.1590/s1516-89132009000600020

Cited by 5 publications

(4 citation statements)

References 24 publications

(19 reference statements)

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“…Augmentin added to the co-culture media was more efficient for bud regeneration than Cefotaxime. The same effect of Augmentin on bud regeneration was described for cotiledonary explants of E. camaldulensis (Quisen et al, 2009) co-cultured with the strain C58C1 of A. tumefaciens, where the antibiotic concentration was reduced from 300 mg.L -1 to 150 mg.L -1 after 15 d and then to 100 mg.L -1 after another 15 d. The number of buds per explant (5.4) was similar to the number obtained in the present study. In peanut (Arachis hypogea), a combination of 200 mg.L -1 Cx and the same concentration of Augmentin ® was recommended to counteract the bacteria growth and to enhance the bud formation (Tiwari and Tuli, 2012).…”

Section: Discussionmentioning

confidence: 80%

“…The response of Eucalyptus to this kind of antibiotic varies with the species (Gonzales et al, 2000). For E. camaldulensis, 9 mg.L -1 Km was sufficient to select transgenic shoots (Mullins et al, 1997;Quisen et al, 2009) while, for E. tereticornis, Prakash and Gurumurthi (2009) established an efficient protocol using 40 mg.L -1 , and reaching 14.4 to 21.2% of transformation efficiency.…”

Section: Discussionmentioning

confidence: 99%

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OPTIMIZATION OF FACTORS AFFECTING THE Agrobacterium tumefaciens- MEDIATED TRANSFORMATION OF Eucalyptus saligna

et al. 2018

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This study aimed to evaluate the effect of factors that may affect the genetic transformation of cotiledonary explants of Eucalyptus saligna mediated by EHA105 strain of Agrobacterium tumefaciens. The vector pBI121 carrying gus gene under control of 35S CaMV promoter was used. The effect of the following factors was evaluated: explant pre-culture, use of different antibiotics and presence of acetosyringone (AS) in co-culture media. An antioxidant solution was also used during excision, containing ascorbic acid (250mg.L-1), citric acid (25mg.L-1) and PVP-40 (1g.L-1). Pre-culture of the explants before the co-culture with bacteria was done over a 4-day period in MS culture medium supplemented with 4.4µM BAP and 2.7ìM NAA. After theco-culture period, three concentrations of kanamycin (12.5;25 and 50mg.L-1) combined with 300mg.L-1 Augmentin® in the culture medium were tested The influence of the antibiotic was also evaluated by keeping the explants in a medium containing 50mg.L-1 Km and 300mg.L-1 Augmentin® or 500mg.L-1 cefotaxime. It was concluded that Augmentin® stimulates organogenesis, that a Km concentration of 12.5mg.L-1 allows selection of explants transformed with gus gene and, finally, the addition of AS (50ìM) to the liquid and solid co-culture media has a positive effect on gus gene expression. Moreover, the use of an antioxidant solution during cotyledon excision is dispensable and the pre-culture of the explants has no effect on bud regeneration or gus gene expression. A transformation efficiency of 1.5% was reached.

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Section: Discussionmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 99%

OPTIMIZATION OF FACTORS AFFECTING THE Agrobacterium tumefaciens- MEDIATED TRANSFORMATION OF Eucalyptus saligna

et al. 2018

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“…No presente trabalho, a interação entre o tipo de meio de cultura e a concentração de TDZ foi significativa para a porcentagem de oxidação, formação de gemas e brotos, e número de brotos. Entretanto, há relatos do efeito positivo do BAP na indução da calogênese, como no caso de Eucalyptus camaldulensis (QUISEN et al, 2009).…”

Section: Resultsunclassified

“…Na organogênese, a influência exercida pela composição dos meios de cultura tem sido estudada em várias espécies do gênero Eucalyptus (QUISEN et al, 2009). Esta influência pode variar em função da composição de macro e micronutrientes do meio de cultura, do uso de agentes antioxidantes, do balanço entre os reguladores de crescimento utilizados, dentre outros.…”

Section: Introductionunclassified

Organogênese indireta a partir de explantes foliares e multiplicação in vitro de brotações de <i>Eucalyptus benthamii</i> X <i>Eucalyptus dunnii</i>

Oliveira-Cauduro¹,

Adamuchio²,

Degenhardt³

et al. 2014

Ciênc. Florest.

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Os objetivos deste trabalho foram avaliar diferentes meios de cultura na organogênese indireta e na multiplicação in vitro de brotos de Eucalyptus benthamii x Eucalyptus dunnii. Para organogênese, explantes foliares foram excisados no sentido transversal e cultivados in vitro, sendo os seguintes fatores testados: dois meios de cultura (MS N/2 e JADS) adicionados de 0,1 µM de ANA, duas concentrações de thidiazuron (0,1 e 0,5 μM) e presença ou não de PVP-40 (250 mg L-1). Após 70 dias de cultivo foram avaliadas as porcentagens de explantes oxidados totalmente, formando calo, produzindo antocianina, formando gema, formando brotações e o número de brotações formadas por explante regenerando. No experimento de multiplicação, brotações isoladas foram cultivadas em meio MS, JADS e WPM, adicionados de 1,11 μM de BAP. Foram realizados quatro subcultivos a cada 28 dias e em cada subcultivo foram avaliados: a porcentagem de oxidação, de explantes apresentando clorose total ou parcial, massa fresca e número médio de brotos por explante. O meio de cultura MS N/2 suplementado com 0,1 µM de ANA, 0,5 µM de TDZ e PVP-40 promoveu a maior taxa de organogênese (8,3%). No meio de cultura MS com 1,11 μM de BAP, a taxa de multiplicação foi maior que nos outros meios, no primeiro e segundo subcultivos (9,28 e 9,24 por mês), não havendo diferença entre os três meios nos demais subcultivos. Palavras-chave: regeneração; espécie lenhosa; regulador vegetal; meio de cultura.

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Reasons and riddance of Agrobacterium tumefaciens overgrowth in plant transformation

Sutradhar

Mandal

2023

Transgenic Res

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Selective agent and A. tumefaciens overgrowth-control antibiotics in Eucalyptus camaldulensis Cotiledonary culture

Abstract: ABSTRACT

Cited by 5 publications

References 24 publications

OPTIMIZATION OF FACTORS AFFECTING THE Agrobacterium tumefaciens- MEDIATED TRANSFORMATION OF Eucalyptus saligna

OPTIMIZATION OF FACTORS AFFECTING THE Agrobacterium tumefaciens- MEDIATED TRANSFORMATION OF Eucalyptus saligna

Organogênese indireta a partir de explantes foliares e multiplicação in vitro de brotações de <i>Eucalyptus benthamii</i> X <i>Eucalyptus dunnii</i>

Reasons and riddance of Agrobacterium tumefaciens overgrowth in plant transformation

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