Morphological and physiological photon flux influence under in vitro culture of apple shoots

Zanandrea, Ilisandra; Bacarin, Marcos Antônio; Braga, Eugênia Jacira Bolacel; Bianchi, Valmor João; Peters, José Antônio

doi:10.1590/s1516-89132009000500005

Cited by 3 publications

(1 citation statement)

References 27 publications

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“…The allocation of carbohydrates was evaluated through the quantification of reducing sugars (RS), total soluble sugars (TSS), and leaf starch, stem above and below the grafting point and roots. The plant tissues used for dry mass evaluation were ground in a Willie-knife mill, model STAR FT-50, stored in a freezer at -18 ° C. The extracts were obtained according to Zanandrea et al (2009), using variable mass, 0.15 g (leaf and stem below the grafting point); 0.1 g (stem below the grafting point), 0.3 g (cultivar root), and 0.4 g (wild plants root).…”

Section: Analysis Of Carbohydratesmentioning

confidence: 99%

Primary metabolism and initial development of grafted black pepper seedlings

Crasque¹,

Arantes²,

Neto³

et al. 2021

RSD

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The production of black pepper seedlings through grafting is a promising strategy to mitigate the effect of environmental stresses, such as drought, that limit its cultivation. However, studies on compatibility between grafts and rootstocks are still scarce, especially considering the wild species of the genus Piper and the cultivated genotypes of black pepper. The objective of this work was to evaluate the relationship between primary metabolism and the development of black pepper seedlings obtained through intra- and interspecific grafting. The method used was that of cleft lateral grafting, as graft the Bragantina cultivar, better known in the world as ‘Panniyur 1’, and ‘Kottanadan’ cultivar and three wild species (Piper hispidum, Piper aduncum and Piper tuberculatum) were used as root-stock. As a control, ´Bragantina` was grafted onto itself (homograft). The experiment was carried out for 110 days and the seedlings were evaluated for development, gas exchange, and carbohydrate allocation. The rootstock influences the development and primary metabolism of seedlings. P. aduncum showed greater initial compatibility among wild species, due to less impediment to carbohydrate flow. P. tuberculatum showed early incompatibility, as the grafts died. The seedlings grafted on P. hispidum and 'Kottanadan' had initial compatibility with an average of 78% survival and 60% budding, but they may have late incompatibility due to limitation of carbohydrate flow.

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Section: Analysis Of Carbohydratesmentioning

confidence: 99%

Primary metabolism and initial development of grafted black pepper seedlings

Crasque¹,

Arantes²,

Neto³

et al. 2021

RSD

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Aspects Regarding the In vitro Propagation of ‘Royal Gala’ Apple Cultivar

Fira¹,

Simu²,

Vlaicu³

et al. 2015

BUASVMCN-HORT

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Apple cultivar 'Royal Gala' was multiplied in vitro on starch-gelled MS media supplemented with 0.7 mg/l BAP, which provided intense proliferation of axillary shoots. In order to test the influence of gelling agents upon proliferation rate in this apple cultivar, modified MS media were used, supplemented with 0.7 mg/l BAP and five types gelling agents: fibrous agar, 6.8 g/l; Phytagel + starch, respectively 0.5 + 50 g/l; guar gum, 20 g/l; Psyllium husk, 15 g/l; Phytagel at 2.2 g/l. The highest proliferation rate, 13.44 ± 1.22 was obtained when the media was gelling with the mixture of Phytagel + starch. By using ex vitro rooting and acclimatization in floating perlite, the rooting percentages were between 99.45% and 93.92%, depending on the culture media used in the multiplication stage, from which the axillary shoots were harvested.

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