Micropropagation and in vitro conservation of Neoglaziovia variegata (Arr. Cam.) mez, a fiber producing bromeliad from Brazil

Silveira, Daniela Garcia; Souza, Fernanda Vidigal Duarte; Pelacani, Claudinéia Regina; Souza, A. da S.; Lêdo, Carlos Alberto da Silva; Santana, José Raniere Ferreira de

doi:10.1590/s1516-89132009000400016

Cited by 29 publications

(25 citation statements)

References 18 publications

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“…In the case of A. distichantha, although germination also began on the fifth day after sowing, the process went on until the 35 th and 40 th day for seeds kept under light and darkness, respectively. Silveira et al (2009), working with Neoglaziovia variegate in the same light conditions we report here, also observed differences in germination rate when studying seeds collected in ripe and immature fruits. These authors reported that germination was favored by light, observing 100% and 80% of radicle protrusion at the 10 th and 15 th days for mature and immature seeds, respectively.…”

Section: Resultssupporting

confidence: 77%

“…Most protocols which use seeds as explant source in bromeliads (Pickens et al, 2006;Bellintani et al, 2008;Silveira et al, 2009;Souza et al, 2009) do not report contamination.…”

Section: Resultsmentioning

confidence: 99%

“…In vitro propagation allows for a quick genetically safe multiplication of a high number of plants, which matches with the large market demand. Studies involving in vitro propagation resulted in the development of multiplication protocols for several species (Mendes et al, 2007;Silva et al, 2008;Silveira et al, 2009;Santos et al, 2010;Huang et al, 2011), including A. blanchetiana (Kanashiro et al, 2007;Galvanesi et al, 2007;Chu et al, 2010).…”

Section: Micropropagação Das Bromélias Ornamentais Vulneráveis Aechmementioning

confidence: 99%

“…Culture medium is one of the major factors, as it paces plant growth and development (Grattapaglia & Machado, 1998). The MS basal medium (Murashige & Skoog, 1962) is the most used for micropropagation in bromeliads, using supplementation with auxins and cytokines, for example naphthaleneacetic acid (NAA), kinetin (KIN) or benzylaminopurine (BAP) at various concentrations, according to the particularities of each step of the micropropagation process (Souza et al, 2003;Droste et al, 2005;Bellintani et al, 2008;Silveira et al, 2009). As in other species, the need to develop specific in vitro protocols for Aechmea blanchetiana and A. distichantha comes mainly from the possibility that a fortuitous genotype-specific dependency appears, which would require optimization procedures to avoid compromising the results.…”

Section: Micropropagação Das Bromélias Ornamentais Vulneráveis Aechmementioning

confidence: 99%

“…Given this, seed production, seems to suit best as propagation strategy for species like Aechmea at this point. Sexual reproduction results in offspring that ensures the preservation of genetic variability and avoids gene loss (Mercier & Nievola, 2003;Carneiro & Mansur, 2004;Silveira et al, 2009).…”

Section: Micropropagação Das Bromélias Ornamentais Vulneráveis Aechmementioning

confidence: 99%

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Micropropagation of the ornamental vulnerable bromeliads Aechmea blanchetiana and Aechmea distichantha

et al. 2013

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Aechmea blanchetiana and Aechmea distichantha are Brazilian native bromeliads with great ornamental value, although none of the species are produced commercially. Therefore, market demands are met through nature predatory exploitation. This study intended to (1) evaluate the in vitro seed germination of A. blanchetiana and A. distichantha and (2) establish micropropagation protocols for both species, aiming at producing plantlets for the market, as well as to germplasm in vitro conservation. Germination experiments were carried out in completely randomized designs with two treatments (presence and absence of light) and seven and eight replications respectively for A. blanchetiana and A. distichantha. In vitro seedlings, produced under light conditions, were used as explants in the multiplication experiment (MS medium). The experimental design was again completely randomized, with treatments in factorial 2 (NAA concentrations; 0.05 and 0.5 μM) x 2 (cytokines: BAP and KIN) x 2 (cytokine levels: 2.2 and 4.4 μM) + 1 (control with basal MS, without growth regulators), with ten replications per treatment. We evaluated fungal and bacterial contamination, total and cumulative seed germination and number of shoots per treatment in each subculture. We observed only fungal contamination and only in A. blanchetiana (14.0%). Light favored in vitro seed germination in both species (99 and 62% respectively for A. blanchetiana and A. distichantha). MS medium + 0.5 μM NAA + 2.2 μM BAP resulted in the highest multiplication rate for both species, with significant contrasts between this treatment and the control in both species (105.62 more shoots in A. blanchetiana; 223.80 in A. distichantha). BAP promoted the formation of rootless shoots, while kinetin favored the formation of roots rather than shoots. A. blanchetiana and A. distichantha showed 90 and 97% of survival respectively in plantlet acclimatization.

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Section: Resultssupporting

confidence: 77%

“…Most protocols which use seeds as explant source in bromeliads (Pickens et al, 2006;Bellintani et al, 2008;Silveira et al, 2009;Souza et al, 2009) do not report contamination.…”

Section: Resultsmentioning

confidence: 99%

Section: Micropropagação Das Bromélias Ornamentais Vulneráveis Aechmementioning

confidence: 99%

Section: Micropropagação Das Bromélias Ornamentais Vulneráveis Aechmementioning

confidence: 99%

Section: Micropropagação Das Bromélias Ornamentais Vulneráveis Aechmementioning

confidence: 99%

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Micropropagation of the ornamental vulnerable bromeliads Aechmea blanchetiana and Aechmea distichantha

et al. 2013

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Photosynthetic apparatus performance in function of the cytokinins used during the in vitro multiplication of Aechmea blanchetiana (Bromeliaceae)

Rosa

Martins

Rodrigues

et al. 2018

Plant Cell Tiss Organ Cult

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Micropropagation of Hohenbergia penduliflora (A. Rich.) Mez. for sustainable production of plant proteases

et al. 2013

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Micropropagation and in vitro conservation of Neoglaziovia variegata (Arr. Cam.) mez, a fiber producing bromeliad from Brazil

Abstract: ABSTRACT

Cited by 29 publications

References 18 publications

Micropropagation of the ornamental vulnerable bromeliads Aechmea blanchetiana and Aechmea distichantha

Micropropagation of the ornamental vulnerable bromeliads Aechmea blanchetiana and Aechmea distichantha

Photosynthetic apparatus performance in function of the cytokinins used during the in vitro multiplication of Aechmea blanchetiana (Bromeliaceae)

Micropropagation of Hohenbergia penduliflora (A. Rich.) Mez. for sustainable production of plant proteases

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