Plant regeneration through somatic embryogenesis of yacón [smallanthus sonchifolius (poepp. and endl.) H. Robinson]

Corrêa, Cynthia Manyra; Oliveira, Graciele Nicolodi de; Astarita, Leandro Vieira; Santarém, Eliane Romanato

doi:10.1590/s1516-89132009000300005

Cited by 13 publications

(4 citation statements)

References 14 publications

(17 reference statements)

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“…The obtained results were consistent with those of Choffe et al [ 17 ]. In a histological analysis Correa et al [ 15 ], revealed that embryogenic calli were more friable and yellowish in appearance than the non-embryogenic calli. However, the embryogenic calli of E. purpurea were dark green and coherent with a faster growth rate than the non-embryogenic calli.…”

Section: Discussionmentioning

confidence: 99%

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Indirect somatic embryogenesis of purple coneflower (Echinacea purpurea (L.) Moench): a medicinal-ornamental plant: evaluation of antioxidant enzymes activity and histological study

Dehestani-Ardakani¹,

Hejazi²,

Aliabad

2020

Mol Biol Rep

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Purple coneflower (Echinacea purpurea (L.) Moench) is a widely used medicinal and ornamental plant. In the present study, the callus embryogenesis was examined using benzyl adenine (BA) at three levels (3, 4, 5 mg L−1), 1-Naphthalene acetic acid (NAA) at three levels (0.1, 0.2 and 0.5 mg L−1) with or without activated charcoal (1 g L−1), coconut milk (50 ml L−1) and casein hydrolysate (50 mg L−1) in the MS (Murashige and Skoog 1962) medium. The embryogenesis indirectly occurred with the production of callus. The calli were observed in three forms: undifferentiated, embryogenic and organogenic. The embryogenic calli were dark green and coherent with a faster growth rate. The highest embryogenesis (100%) and embryonic regeneration (plantlet production) were obtained in the combined BA + NAA treatments with the activated charcoal, coconut milk and casein hydrolysate. However, the combined treatments of growth regulators failed to produce somatic embryos without the use of coconut milk and casein hydrolysate. The maximum amount of protein, peroxidase and catalase activity of embryogenic calli (2.02, 1.79 and 6.62ΔOD/Min/mg.protein, respectively), and highest percentage of acclimatization success (29.3% of plants) were obtained in the combined treatment of 5 mg L−1 BA + 0.5 mg L−1 NAA + activated charcoal + coconut milk + casein hydrolysate. The highest amount of chlorophyll content (33.3 SPAD value) and growth characteristics of acclimatized plantlets were observed in the media containing 3 mg L−1 BA + 0.1 and 0.2 mg L−1 NAA + 1 g. L−1 combined activated charcoal, coconut milk, casein hydrolysate. The histological studies confirmed the somatic embryogenesis in purple coneflower. Generally, it was found that the somatic embryogenesis of E. purpurea occurs at high levels of BA and low levels of NAA with the addition of coconut milk and casein hydrolysate.

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Section: Discussionmentioning

confidence: 99%

“…and Endl.) H. Robinson] [ 15 ].The sensitivity of the explant tissue to the PGRs may have been altered by the dark treatment, thereby resulting in a higher frequency of embryogenesis [ 12 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Indirect somatic embryogenesis of purple coneflower (Echinacea purpurea (L.) Moench): a medicinal-ornamental plant: evaluation of antioxidant enzymes activity and histological study

Dehestani-Ardakani¹,

Hejazi²,

Aliabad

2020

Mol Biol Rep

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“…24326/ asphc.2022.1.11 the prebiotic compounds, yacon contains flavonoids, phenolic acids and the amino acid tryptophan, which possess antioxidant, anti-inflammatory, antibacterial, and anticarcinogenic properties. Phenolic compounds in yacon protect biomolecules such as DNA, lipids or proteins from damage caused by free oxygen and nitrogen radicals [Corrêa et al 2009].…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, yacon is mostly reproduced vegetatively by cuttings or tuber division. However, these reproduction methods may cause bacterial and viral pathogen transfer [Corrêa et al 2009]. The threat of disease transmission at the vegetative propagation poses a necessity to elaborate a method of in vitro multiplication.…”

Section: Introductionmentioning

confidence: 99%

Elaboration of micropropagation system of medicinal plant yacon (Smallanthus sonchifolius (Poepp.) H. Rob.)

Kiszczak¹,

Kowalska²,

Burian³

et al. 2022

asphc

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Yacon becomes an important plant with strong medical and dietary potential. Therefore, studies on the optimization of yacon micropropagation were undertaken. Reproduction of yacon is conducted by traditional methods with green seedlings or long offsets taken from the underground and aboveground rootstock. The influence of different disinfection methods, various initial explants and growth regulators, their concentrations, and combinations on the efficiency of micropropagation have been analyzed. The most effective disinfection was the use of 70% ethanol and 0.5% Tween 20. The optimal initial explants were apical and lateral buds cultured on Murashige and Skoog (MS) medium with 0.2 mg L–1 kinetin and 1 mg L–1 indole-3-acetic acid. The most intensive shoot regeneration occurred on MS medium with 1 mg L–1 thidiazuron and 1-naphthylacetic acid. Histological observations demonstrated the strong effect of growth substances on shoot organogenesis from callus tissue. Shoots obtained from in vitro cultures rooted easily on modified MS media with ½ or ¼ concentration of macroelements, 20 g L–1 sucrose and 3 mg L–1 1-naphthylacetic acid. All plants adapted to ex vitro conditions.

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Optimizing Factors Affecting Somatic Embryogenesis in Cineraria

Sivanesan

Jeong

2015

Somatic Embryogenesis in Ornamentals and Its Applications

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Plant regeneration through somatic embryogenesis of yacón [smallanthus sonchifolius (poepp. and endl.) H. Robinson]

Abstract: Smallanthus sonchifolius has tuberous roots containing large amounts of fructo-oligosaccharides and its medicinal

Cited by 13 publications

References 14 publications

Indirect somatic embryogenesis of purple coneflower (Echinacea purpurea (L.) Moench): a medicinal-ornamental plant: evaluation of antioxidant enzymes activity and histological study

Indirect somatic embryogenesis of purple coneflower (Echinacea purpurea (L.) Moench): a medicinal-ornamental plant: evaluation of antioxidant enzymes activity and histological study

Elaboration of micropropagation system of medicinal plant yacon (Smallanthus sonchifolius (Poepp.) H. Rob.)

Optimizing Factors Affecting Somatic Embryogenesis in Cineraria

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