Optimizing Factors Affecting Somatic Embryogenesis in Cineraria

Sivanesan, Iyyakkannu; Jeong, Byoung Ryong

doi:10.1007/978-81-322-2683-3_4

Cited by 2 publications

(5 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The in vitro-developed plantlets of E. purpurea were acclimatized after transplanting with an 8.66–29.3% survival rate. Sivanesan and Jeong [ 11 ] reported that 98% of cineraria plantlets were successfully acclimatized in the greenhouse. The in vitro conditions including low light intensity, levels of sucrose and other nutrients, and high relative humidity may cause physiological and anatomical changes that have negative effects on the acclimatization of regenerated plants and explain the limited acclimatization efficiency in this study [ 56 ].…”

Section: Discussionmentioning

confidence: 99%

“…Also, in some cases, it is superior to other methods of asexual propagation, as it allows the mass propagation of plants using the bioreactors [ 9 , 10 ]. Plant growth regulators (PGRs) play an important role in the induction and development of somatic embryogenesis [ 11 ]. Auxin is often required for the induction of somatic embryogenesis from different explants, and the process of somatic embryogenesis is generally initiated on the culture medium supplemented with high concentrations of auxins [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

“…Plant growth regulators (PGRs) play an important role in the induction and development of somatic embryogenesis [ 11 ]. Auxin is often required for the induction of somatic embryogenesis from different explants, and the process of somatic embryogenesis is generally initiated on the culture medium supplemented with high concentrations of auxins [ 11 ]. However, somatic embryogenesis has been induced on auxin-free media in various plants [ 12 – 14 ].…”

Section: Introductionmentioning

confidence: 99%

“…However, somatic embryogenesis has been induced on auxin-free media in various plants [ 12 – 14 ]. In several plants, a combination of auxin and cytokinin stimulates the development of somatic embryogenesis [ 11 ]. The use of 2,4-D (4.5 μM) and BA (0.45 μM) for the induction of somatic embryogenesis has been reported for yacon [ Smallanthus sonchifolius (Poepp.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Indirect somatic embryogenesis of purple coneflower (Echinacea purpurea (L.) Moench): a medicinal-ornamental plant: evaluation of antioxidant enzymes activity and histological study

Dehestani-Ardakani¹,

Hejazi²,

Aliabad

2020

Mol Biol Rep

View full text Add to dashboard Cite

Purple coneflower (Echinacea purpurea (L.) Moench) is a widely used medicinal and ornamental plant. In the present study, the callus embryogenesis was examined using benzyl adenine (BA) at three levels (3, 4, 5 mg L−1), 1-Naphthalene acetic acid (NAA) at three levels (0.1, 0.2 and 0.5 mg L−1) with or without activated charcoal (1 g L−1), coconut milk (50 ml L−1) and casein hydrolysate (50 mg L−1) in the MS (Murashige and Skoog 1962) medium. The embryogenesis indirectly occurred with the production of callus. The calli were observed in three forms: undifferentiated, embryogenic and organogenic. The embryogenic calli were dark green and coherent with a faster growth rate. The highest embryogenesis (100%) and embryonic regeneration (plantlet production) were obtained in the combined BA + NAA treatments with the activated charcoal, coconut milk and casein hydrolysate. However, the combined treatments of growth regulators failed to produce somatic embryos without the use of coconut milk and casein hydrolysate. The maximum amount of protein, peroxidase and catalase activity of embryogenic calli (2.02, 1.79 and 6.62ΔOD/Min/mg.protein, respectively), and highest percentage of acclimatization success (29.3% of plants) were obtained in the combined treatment of 5 mg L−1 BA + 0.5 mg L−1 NAA + activated charcoal + coconut milk + casein hydrolysate. The highest amount of chlorophyll content (33.3 SPAD value) and growth characteristics of acclimatized plantlets were observed in the media containing 3 mg L−1 BA + 0.1 and 0.2 mg L−1 NAA + 1 g. L−1 combined activated charcoal, coconut milk, casein hydrolysate. The histological studies confirmed the somatic embryogenesis in purple coneflower. Generally, it was found that the somatic embryogenesis of E. purpurea occurs at high levels of BA and low levels of NAA with the addition of coconut milk and casein hydrolysate.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Indirect somatic embryogenesis of purple coneflower (Echinacea purpurea (L.) Moench): a medicinal-ornamental plant: evaluation of antioxidant enzymes activity and histological study

Dehestani-Ardakani¹,

Hejazi²,

Aliabad

2020

Mol Biol Rep

View full text Add to dashboard Cite

show abstract

“…Existing research on Crocus tissue culture demonstrates a variety of successful approaches. Demeter et al (2010) achieved embryogenic calli in C. heuffelianus, while Sivanesan et al (2012Sivanesan et al ( , 2014 (Freytag et al 2017). However, the application of these diverse techniques to C. scepusiensis seems less explored.…”

Section: Introductionmentioning

confidence: 99%

Phytochemical characterization of callus cultures from the endangered plant Crocus scepusiensis (Rehm. & Woł.) Borbás ex Kulcz.

Khlifa,

Sweelam,

El-Desoky

et al. 2024

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

Crocus scepusiensis (Rehm. & Woł.) Borbás ex Kulcz., a critically endangered herbaceous plant which serves as a valuable source of bioactive compounds found across Europe and Asia. The aim of this study was to produce a calli from two different plant parts (leaf and shoot tip) for the critically endangered C. scepusiensis through tissue culture techniques, characterize the resulting calli through chemical profiling, with a focus on identifying key phytoconstituents, and lay the groundwork for future research on the biological activities of these calli extracts. Leaf disc and micro shoot tip explants were cultured on Murashige and Skoog (MS) medium supplemented with various concentrations of 6-benzylaminopurine (BA) and α-naphthaleneacetic acid (NAA) to induce organogenic calli. The resulting calli exhibited distinct biochemical profiles. Moreover, a phytochemical analysis was conducted to compare the metabolite composition of callus 1 (derived from leaf discs) and callus 2 (derived from shoot tips). Callus 1 displayed a higher total phenolic content (30.3558 ± 1.3564 mg (GAE)/g) compared to callus 2 (29.1543 ± 0.9754 mg (GAE)/g). Similarly, callus 1 exhibited a greater total flavonoid content (26.0089 ± 1.8029 mg (RE)/g) than callus 2 (18.4464 ± 1.4797 mg (RE)/g). Liquid chromatography-photodiode array-electrospray ionization-tandem mass spectrometry (LC-PDA-ESI-MS/MS) analysis revealed the presence of 26 and 25 constituents in callus 1 and 2, respectively. Fourteen and thirteen of these identified compounds have been previously reported in other Crocus species, with 22 constituents common to both calli. Twelve constituents were reported here in Crocus for the first time as far as we know.

show abstract

Optimizing Factors Affecting Somatic Embryogenesis in Cineraria

Cited by 2 publications

References 43 publications

Indirect somatic embryogenesis of purple coneflower (Echinacea purpurea (L.) Moench): a medicinal-ornamental plant: evaluation of antioxidant enzymes activity and histological study

Indirect somatic embryogenesis of purple coneflower (Echinacea purpurea (L.) Moench): a medicinal-ornamental plant: evaluation of antioxidant enzymes activity and histological study

Phytochemical characterization of callus cultures from the endangered plant Crocus scepusiensis (Rehm. & Woł.) Borbás ex Kulcz.

Contact Info

Product

Resources

About