Evaluation of emulsifier stability of biosurfactant produced by Saccharomyces lipolytica CCT-0913

Abstract

Surface-active compounds of biological origin are widely used for many industries (cosmetic, food, petrochemical). The Saccharomyces lipolytica CCT-0913 was able to grow and produce a biosurfactant on 5% (v/v) diesel-oil at pH 5.0 and 32 o C. The cell-free broth emulsified and stabilized the oil-in-water emulsion through a first order kinetics. The results showed that the initial pH value and temperature influenced the emulsifier stability (ES), which was the time when oil was separated. The biosurfactant presented different stabilization properties for vegetable and mineral oil in water solution, despite the highest values of the ES occurring with vegetable oil. The biosurfactantpresented smallest ES when compared to commercial surfactants; however, this biosurfactant was not purified.

Isolation and characterization of a biosurfactant‐producing Fusarium sp. BS‐8 from oil contaminated soil

“…Biosurfactant production at 30°C from different fungal and yeast strains was reported by many scientists, for example, C. antarctica , 34 , C. bombicola , Ustilago scitaminea , and Pseudozyma species . It has been observed that some microorganisms preferred a pH range of 5 to 7 for optimum biosurfactant production . The findings in this study were concurrent with previous reports that indicated the biosurfactant production by fungal species when cultivated in the range of temperature 20–30°C, pH 5–7 and agitation speed of 100–200 rpm …”

“…7,37 It has been observed that some microorganisms preferred a pH range of 5 to 7 for optimum biosurfactant production. 38,39 The findings in this study were concurrent with previous reports that indicated the biosurfactant production by fungal species when cultivated in the range of temperature 20-30 C, pH 5-7 and agitation speed of 100-200 rpm. 32,33 The effect of different nutritional factors on biosurfactant production by Fusarium sp.…”

“…38,45 The results of present study are in full agreement with previous reports by other scientists who reported the production of biosurfactant by Aspergillus species in the presence of yeast extract. 23 Other fungal isolates were able to produce biosurfactant in the presence of other nitrogen sources such as Pseudozyma species 7,37 in the presence of NaNO 3 , C. glabrata UCP1002 38 in the presence of NH 4 NO 3 and Saccharomyces lipolytica 39 in the presence of NH 4 Cl. The nitrogen source influenced the biosurfactant production because it is essential for protein and enzyme synthesis, which in turn, are needed for microbial growth.…”

“…Thus, the oil contaminated sites are envisaged as enriched source of biosurfactant‐producing microorganisms . So far, most of research in the field of biosurfactants has been carried out using bacteria and yeasts, however, biosurfactants from different filamentous fungi have also been reported by a few scientists . Unlike other oleaginous microbes, filamentous fungi are considered as dynamic mini‐eukaryotic biofactories for production of many industrially important biotechnological products, including commercial enzymes, organic acids, alkaloids, plant growth regulators, antibiotics, polysaccharides, and pigments .…”

Emulsifying Properties of Lecithin Containing Different Fatty Acids Obtained by Immobilized Lecitase Ultra‐Catalyzed Reaction

“…Emulsifying activity (EA) and emulsion stability (ES) are used to measure emulsifying properties and have been widely applied in evaluating emulsifiers [7][8][9]. EA is commonly expressed as the turbidity of emulsion immediately after preparation, whereas ES generally measures the amount of oil and/or cream separating from an emulsion over a short period, depending on the oil concentration, gravitational field, and temperature [10]. Turbiscan Lab Ò Expert is an innovative instrument that has recently been used for the evaluation of emulsion stability [11][12][13].…”