Jacaratia corumbensis O. Kuntze a new vegetable source for milk-clotting enzymes

Duarte, Ana Rodrigues; Duarte, Débora Maria Rodrigues; Moreira, Keila Aparecida; Cavalcanti, Maria Taciana Holanda; Lima-Filho, José Luiz de; Porto, Ana Lúcia Figueiredo

doi:10.1590/s1516-89132009000100001

Cited by 37 publications

(28 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Optimal temperature of the enzymatic activity of A. precatorius extract was 40˚C as on the viscera of Tilapia nilotica [7]. The result was different to that reported by Duarte et al [22] who indicated 55˚C as optimal temperature from proteases of Jacaratia corumbensis extract.…”

Section: Proteolytic Activity Of Crude and Partially Purified Extractscontrasting

confidence: 76%

Partial Purification and Characterization of Protease from <i>Abrus precatorius</i> Linn. (Fabaceae) from Cameroon

Blandine¹,

Serge²,

Tchiégang³

2016

AER

View full text Add to dashboard Cite

Crude enzyme extracts were prepared from leaves and stems of Abrus precatorius Linn. (Fabaceae) from Cameroon under optimized conditions. Proteolytic enzymes were precipitated with ammonium sulfate at 35% (w/v) saturation and assayed for enzyme activity. The effects of temperature, pH, incubation time and substrate specificity were studied. SDS-PAGE was used to determine molecular weight of precipitated protease. Results indicated that proteolytic activity of crude extract was 35.20 U/ml compared to 51.03 U/ml of partial purified extract. The optimum enzyme activity was found to be at 40˚C, while 50% of activity was maintained at 60˚C after 60 min incubation. Partial purified crude extract exhibited two optimum pH (2.75 and 9.0). The highest enzyme activity towards Bovine Serum Albumine (25.9 U/ml) was noted. SDS-PAGE gels exhibited molecular weight between 40 -60 KDa. This result confirms that partial purified extract of A. precatorius contains proteases and could be a promising source for proteolytic enzyme extraction.

show abstract

Section: Proteolytic Activity Of Crude and Partially Purified Extractscontrasting

confidence: 76%

Partial Purification and Characterization of Protease from <i>Abrus precatorius</i> Linn. (Fabaceae) from Cameroon

Blandine¹,

Serge²,

Tchiégang³

2016

AER

View full text Add to dashboard Cite

show abstract

“…However, this method is ethically arguable and has low productivity. Thus, research has focused on the identification of alternative milk-clotting enzymes (MCEs) among animals, plants and microorganisms as calf rennet substitutes [2][3][4][5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

A two-stage oxygen supply control strategy for enhancing milk-clotting enzyme production by Bacillus amyloliquefaciens

Ding

Ai²,

Ouyang

et al. 2012

Eur Food Res Technol

View full text Add to dashboard Cite

To enhance the yield and productivity of milkclotting enzyme (MCE) by Bacillus amyloliquefaciens, a two-stage oxygen supply control strategy was proposed and successfully applied in the MCE fermentation. During the first 16 h, K L a was controlled at 72.2 h -1 to obtain high cell growth rate (v) and MCE activity (MCA) productivity (r MCA ). Subsequently, K L a was controlled at 33.9 h -1 to maintain high specific MCA productivity (q MCA ). Using this strategy, MCA peaked at 36 h with the MCA of 6,590.41 SU ml -1 , which was 18 h earlier than other investigated processes. The concept and results described represent the basis of an industrial scale-up process to achieve high MCE yield, MCA productivity and MCA/proteolytic activity.

show abstract

“…Folhas, frutos, tubér-culos e raizes geralmente são faceis de serem rompidos usando um moinho, por exemplo. Por outro lado, cascas, caules e sementes possuem paredes celulares rigidas e requerem equipamentos mais específicos (19).…”

Section: Métodos De Obtenção De Amostras E Enzimas Vegetaisunclassified

Enzimas Vegetais: Extração E Aplicações Biotecnológicas

Antunes

Lopes

Brito

et al. 2017

Infarma

View full text Add to dashboard Cite

The manuscript describes the current analytical methodologies used in obtaining plant enzymes for biotechnological applications. The isolation and partial purification methods are reported, summarized in chromatographic electrophoretic techniques. The importance of these enzymes in biotechnology research, with a focus on its applications, characteristics and peculiarities, are emphasizing either. With regard to the applicability, the article brings several examples in food production, manufacturing and production of biosensors for determination of various analytes. About bioremediation, we aimed on pollutant removal of the natural environment and its conversion into less harmful products, using those plant enzymes. Industrial interests are also discussed.Keywords: biotechnology; food production; biosensing techniques; bioremediation. RESUMOO artigo descreve as metodologias analíticas atuais mais utilizadas na obtenção de enzimas vegetais para aplicações biotecnológicas. São relatados os métodos de isolamento e purificação parcial, que se resumem nas técnicas cromatográficas e eletroforéticas, enfatizando a importância dessas enzimas nas pesquisas em Biotecnologia, com foco nas suas aplicações, características e peculiaridades. No que se refere à aplicabilidade, o artigo traz diversos exemplos na produção de alimentos, na fabricação e produção de biossenssores para determinação de diversos analitos e na biorremediação, que que tem como objetivo a remoção de poluentes do ambiente natural e a sua conversão em produtos menos prejudiciais, utilizando, para isso, as enzimas vegetais. Interesses industriais também são discutidos.Palavras-chave: produção de alimentos; técnicas biossensoriais; biorremediação.Revisão 10.14450/2318-9312.v29.e3.a2017.pp181-198 INTRODUÇÃO A Biotecnologia é o conjunto de técnicas que permitem gerar produtos de interesse econômico e social a partir de organismos vivos ou de seus componentes (1). Nas últimas décadas apresentou uma expansão de aplicações bem sucedidas em produtos e técnicas, principalmente aquelas envolvendo enzimas (2). As enzimas são consideradas catalisadores biológicos, por exercerem a função de acelerar ou mesmo possibilitar reações entre componentes químicos (3). Estão presentes em todos os sistemas biológicos, são produzidas por todos os organismos vivos e são responsáveis por vários processos metabólicos (4).As enzimas são o principal alvo da pesquisa em Biotecnologia, não apenas por seu papel crucial nos me-

show abstract

Jacaratia corumbensis O. Kuntze a new vegetable source for milk-clotting enzymes

Cited by 37 publications

References 19 publications

Partial Purification and Characterization of Protease from <i>Abrus precatorius</i> Linn. (Fabaceae) from Cameroon

Partial Purification and Characterization of Protease from <i>Abrus precatorius</i> Linn. (Fabaceae) from Cameroon

A two-stage oxygen supply control strategy for enhancing milk-clotting enzyme production by Bacillus amyloliquefaciens

Enzimas Vegetais: Extração E Aplicações Biotecnológicas

Contact Info

Product

Resources

About

Jacaratia corumbensis O. Kuntze a new vegetable source for milk-clotting enzymes

Cited by 37 publications

References 19 publications

Partial Purification and Characterization of Protease from &lt;i&gt;Abrus precatorius&lt;/i&gt; Linn. (Fabaceae) from Cameroon

Partial Purification and Characterization of Protease from &lt;i&gt;Abrus precatorius&lt;/i&gt; Linn. (Fabaceae) from Cameroon

A two-stage oxygen supply control strategy for enhancing milk-clotting enzyme production by Bacillus amyloliquefaciens

Enzimas Vegetais: Extração E Aplicações Biotecnológicas

Contact Info

Product

Resources

About

Partial Purification and Characterization of Protease from <i>Abrus precatorius</i> Linn. (Fabaceae) from Cameroon

Partial Purification and Characterization of Protease from <i>Abrus precatorius</i> Linn. (Fabaceae) from Cameroon