Effect of the extract of Ricinus communis L. on the osmotic fragility, labeling of red blood cells with Technetium-99m and morphology of the cells

Abstract

The aim of this study was to evaluate the influence of the proteic extract of R. communis on the cell physiology by the osmotic fragility, labeling of the blood elements with the 99mTc and cell morphology. To evaluate the osmotic fragility, the blood samples of the Wistar rats were incubated with the concentrations of R. communis and with the solutions of NaCl (0.4; 0.7; 0.9%). In the labeling of the blood elements procedure, the rat blood was treated with a solution of determining the rate of radioactivity (%ATI) in the plasma (P) and in the red blood cells (RBC). The soluble and insoluble fractions of the plasma were also evaluated. The cells morphology submitted to the extract was evaluated by the optical microscopy (x40

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“…This observation may be attributed to the molecules on the cell surface of RBCs which may be directly or indirectly involved in the alteration in the functioning of the ion channels or other mechanisms involved in maintaining the osmotic balance. Similar results were observed when RBCs were exposed to extract of Ricinus communis L., where the extract proved to be toxic to cells and modified the cell membrane structure 7 and exposure to extract of Combretum zeyheri caused hemolysis in sheep erythrocytes 8 .…”

Phytocomponents from Anacardium occidentale, Psidium guajava, and Terminalia catappa altered membrane osmotic stability of sickle erythrocytes

“…Previous studies had reported distortions in membrane osmotic stability of human erythrocyte genotypes, in health and disease, and the capacities of plant extracts to reverse those alterations [26,42,44,[57][58][59]. Certain phytocomponents are associated with stabilization of erythrocyte membrane integrity or, on the contrary, cause exacerbated hemolysis in hypotonic solutions [44,[60][61][62]. For instance, certain classes of the tannins, aminoglycosides, and saponins from verse collections of plant species have been implicated in eliciting erythrocyte membrane destabilization by their chaotropic interaction with biomembrane components [1,21,57,[62][63][64].…”